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Article: In vivo MRI of endogenous stem/progenitor cell migration from subventricular zone in normal and injured developing brains

TitleIn vivo MRI of endogenous stem/progenitor cell migration from subventricular zone in normal and injured developing brains
Authors
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ynimg
Citation
Neuroimage, 2009, v. 48 n. 2, p. 319-328 How to Cite?
AbstractUnderstanding the alterations of migratory activities of the endogenous neural stem/progenitor cells (NSPs) in injured developing brains is becoming increasingly imperative for curative reasons. In this study, 10-day-old neonatal rats with and without hypoxic-ischemic (HI) insult at postnatal day 7 were injected intraventricularly with micron-sized iron oxide particles (MPIOs), followed by serial high-resolution MRI at 7 T for 2 weeks. MRI findings were correlated to the histological analysis using iron staining and several immunohistochemical double staining. The results indicated that in normal and HI-injured brains the NSPs from the subventricular zone (SVZ) were labeled by MPIOs, and migrated as newly created cells (iron+/BrdU+), neuroblasts (iron+/nestin+), astrocytes or astrocytes-like progenitor cells (iron+/GFAP+), and mature neurons (iron+/NeuN+). In normal brains, the endogenous NSPs mainly exhibited a tangential pattern in both rostral and caudal directions. The NSP radial migratory pattern could be observed in some rats. In the HI-injured brains during the same developmental period, the NSPs mainly migrated towards the HI lesion sites. The tangential, rostrocaudal migrations could be observed but impaired. These findings suggest that the NSP migratory pathways in SVZ change in response to the HI insult, likely due to the self-repairing efforts known in the neonatal brains. The MRI approach demonstrated here is potentially applicable to the in vivo and longitudinal study of NSP cell activities in developing brains under normal and pathological conditions and in therapeutic interventions. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/129231
ISSN
2015 Impact Factor: 5.463
2015 SCImago Journal Rankings: 4.464
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilHKU 7793/08M
Chinese National Science Foundation Council30770673
Funding Information:

This work was supported in part by research grants from the Hong Kong Research Grant Council (HKU 7793/08M) and the Chinese National Science Foundation Council (30770673).

References

 

DC FieldValueLanguage
dc.contributor.authorYang, Jen_HK
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorNiu, Gen_HK
dc.contributor.authorChan, KCen_HK
dc.contributor.authorWang, Ren_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2010-12-23T08:33:47Z-
dc.date.available2010-12-23T08:33:47Z-
dc.date.issued2009en_HK
dc.identifier.citationNeuroimage, 2009, v. 48 n. 2, p. 319-328en_HK
dc.identifier.issn1053-8119en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129231-
dc.description.abstractUnderstanding the alterations of migratory activities of the endogenous neural stem/progenitor cells (NSPs) in injured developing brains is becoming increasingly imperative for curative reasons. In this study, 10-day-old neonatal rats with and without hypoxic-ischemic (HI) insult at postnatal day 7 were injected intraventricularly with micron-sized iron oxide particles (MPIOs), followed by serial high-resolution MRI at 7 T for 2 weeks. MRI findings were correlated to the histological analysis using iron staining and several immunohistochemical double staining. The results indicated that in normal and HI-injured brains the NSPs from the subventricular zone (SVZ) were labeled by MPIOs, and migrated as newly created cells (iron+/BrdU+), neuroblasts (iron+/nestin+), astrocytes or astrocytes-like progenitor cells (iron+/GFAP+), and mature neurons (iron+/NeuN+). In normal brains, the endogenous NSPs mainly exhibited a tangential pattern in both rostral and caudal directions. The NSP radial migratory pattern could be observed in some rats. In the HI-injured brains during the same developmental period, the NSPs mainly migrated towards the HI lesion sites. The tangential, rostrocaudal migrations could be observed but impaired. These findings suggest that the NSP migratory pathways in SVZ change in response to the HI insult, likely due to the self-repairing efforts known in the neonatal brains. The MRI approach demonstrated here is potentially applicable to the in vivo and longitudinal study of NSP cell activities in developing brains under normal and pathological conditions and in therapeutic interventions. © 2009 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ynimgen_HK
dc.relation.ispartofNeuroImageen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAstrocytes - physiologyen_HK
dc.subject.meshBrain - growth & development - physiology - physiopathologyen_HK
dc.subject.meshBromodeoxyuridineen_HK
dc.subject.meshCell Movement - physiologyen_HK
dc.subject.meshFerric Compoundsen_HK
dc.subject.meshGlial Fibrillary Acidic Protein - metabolismen_HK
dc.subject.meshHypoxia-Ischemia, Brain - physiopathologyen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshIntermediate Filament Proteins - metabolismen_HK
dc.subject.meshIron - metabolismen_HK
dc.subject.meshMagnetic Resonance Imagingen_HK
dc.subject.meshNerve Tissue Proteins - metabolismen_HK
dc.subject.meshNeurons - physiologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshStem Cell Niche - physiologyen_HK
dc.subject.meshStem Cells - physiologyen_HK
dc.subject.meshTime Factorsen_HK
dc.titleIn vivo MRI of endogenous stem/progenitor cell migration from subventricular zone in normal and injured developing brainsen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.neuroimage.2009.06.075en_HK
dc.identifier.pmid19591946-
dc.identifier.scopuseid_2-s2.0-68949184821en_HK
dc.identifier.hkuros177186en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68949184821&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume48en_HK
dc.identifier.issue2en_HK
dc.identifier.spage319en_HK
dc.identifier.epage328en_HK
dc.identifier.isiWOS:000274723900002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYang, J=10041733800en_HK
dc.identifier.scopusauthoridLiu, J=8345637700en_HK
dc.identifier.scopusauthoridNiu, G=35273273000en_HK
dc.identifier.scopusauthoridChan, KC=34968940300en_HK
dc.identifier.scopusauthoridWang, R=36071507000en_HK
dc.identifier.scopusauthoridLiu, Y=35196202900en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK
dc.identifier.citeulike5220206-

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