Article: Revisit complexation between DNA and polyethylenimine - Effect of uncomplexed chains free in the solution mixture on gene transfection
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- Publisher Website: 10.1016/j.jconrel.2010.10.028
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- PMID: 21056067
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| Title | Revisit complexation between DNA and polyethylenimine - Effect of uncomplexed chains free in the solution mixture on gene transfection | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Yue, Y3 Jin, F3 Deng, R3 Cai, J3 Chen, Y3 Lin, MCM1 Kung, HF3 Wu, C2 3 | ||||||
| Keywords | Cellular internalization DNA condensation Free polyethylenimine Gene delivery Laser light scattering | ||||||
| Issue Date | 2011 | ||||||
| Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel | ||||||
| Citation | Journal Of Controlled Release, 2011, v. 155 n. 1, p. 67-76 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.jconrel.2010.10.028 | ||||||
| Abstract | Our revisit of the complexation between anionic DNA and cationic polyethylenimine (PEI) in both water and phosphate buffered saline (PBS) by using a combination of laser light scattering (LLS) and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~ 3, but the PEI/DNA polyplexes have a high in-vitro gene transfection efficiency only when N:P ≥ 10. Putting these two facts together, we not only conclude that this extra 7 portions of PEI chains are free in the solution mixture, but also confirmed that it is these free PEI chains that substantially promote the gene transfection no matter whether they are applied hours before or after the administration of the much less effective PEI/DNA polyplexes (N:P = 3). The uptake kinetics measured by flow cytometry shows that the addition of free PEI leads to a faster and more efficient cellular internalization of polyplexes, but these free PEI chains mainly contribute to the subsequent intracellular trafficking. In contrast, the bound PEI chains mainly play a role in the DNA condensation and protection, leading to a different thinking in the development of non-viral vectors. © 2010 Elsevier B.V. All rights reserved. | ||||||
| ISSN | 0168-3659 2011 Impact Factor: 5.732 2011 SCImago Journal Rankings: 0.548 | ||||||
| DOI | http://dx.doi.org/10.1016/j.jconrel.2010.10.028 | ||||||
| ISI Accession Number ID | WOS:000295998200012
Funding Information: The financial support of the National Natural Scientific Foundation of China (NNSFC) Projects (50773077 and 20934005) and the Hong Kong Special Administration Region Earmarked (RGC) Projects (CUHK4037/07P, 2160331; CUHK4046/08P, 2160365; CUHK4039/08P, 2160361; and CUHK4042/09P, 2160396) is gratefully acknowledged. | ||||||
| References | References in Scopus |
| dc.contributor.author | Yue, Y | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Jin, F | ||||||
| dc.contributor.author | Deng, R | ||||||
| dc.contributor.author | Cai, J | ||||||
| dc.contributor.author | Chen, Y | ||||||
| dc.contributor.author | Lin, MCM | ||||||
| dc.contributor.author | Kung, HF | ||||||
| dc.contributor.author | Wu, C | ||||||
| dc.date.accessioned | 2010-12-23T08:32:44Z | ||||||
| dc.date.available | 2010-12-23T08:32:44Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | Our revisit of the complexation between anionic DNA and cationic polyethylenimine (PEI) in both water and phosphate buffered saline (PBS) by using a combination of laser light scattering (LLS) and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~ 3, but the PEI/DNA polyplexes have a high in-vitro gene transfection efficiency only when N:P ≥ 10. Putting these two facts together, we not only conclude that this extra 7 portions of PEI chains are free in the solution mixture, but also confirmed that it is these free PEI chains that substantially promote the gene transfection no matter whether they are applied hours before or after the administration of the much less effective PEI/DNA polyplexes (N:P = 3). The uptake kinetics measured by flow cytometry shows that the addition of free PEI leads to a faster and more efficient cellular internalization of polyplexes, but these free PEI chains mainly contribute to the subsequent intracellular trafficking. In contrast, the bound PEI chains mainly play a role in the DNA condensation and protection, leading to a different thinking in the development of non-viral vectors. © 2010 Elsevier B.V. All rights reserved. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Journal Of Controlled Release, 2011, v. 155 n. 1, p. 67-76 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.jconrel.2010.10.028 | ||||||
| dc.identifier.citeulike | 8215993 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.jconrel.2010.10.028 | ||||||
| dc.identifier.epage | 76 | ||||||
| dc.identifier.hkuros | 183297 | ||||||
| dc.identifier.isi | WOS:000295998200012
Funding Information: The financial support of the National Natural Scientific Foundation of China (NNSFC) Projects (50773077 and 20934005) and the Hong Kong Special Administration Region Earmarked (RGC) Projects (CUHK4037/07P, 2160331; CUHK4046/08P, 2160365; CUHK4039/08P, 2160361; and CUHK4042/09P, 2160396) is gratefully acknowledged. | ||||||
| dc.identifier.issn | 0168-3659 2011 Impact Factor: 5.732 2011 SCImago Journal Rankings: 0.548 | ||||||
| dc.identifier.issue | 1 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmid | 21056067 | ||||||
| dc.identifier.scopus | eid_2-s2.0-79953839673 | ||||||
| dc.identifier.spage | 67 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/129117 | ||||||
| dc.identifier.volume | 155 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel | ||||||
| dc.publisher.place | Netherlands | ||||||
| dc.relation.ispartof | Journal of Controlled Release | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject | Cellular internalization | ||||||
| dc.subject | DNA condensation | ||||||
| dc.subject | Free polyethylenimine | ||||||
| dc.subject | Gene delivery | ||||||
| dc.subject | Laser light scattering | ||||||
| dc.title | Revisit complexation between DNA and polyethylenimine - Effect of uncomplexed chains free in the solution mixture on gene transfection | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- University of Science and Technology of China
- Chinese University of Hong Kong