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Article: Revisit complexation between DNA and polyethylenimine - Effect of uncomplexed chains free in the solution mixture on gene transfection
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TitleRevisit complexation between DNA and polyethylenimine - Effect of uncomplexed chains free in the solution mixture on gene transfection
 
AuthorsYue, Y3
Jin, F3
Deng, R3
Cai, J3
Chen, Y3
Lin, MCM1
Kung, HF3
Wu, C3 2
 
KeywordsCellular internalization
DNA condensation
Free polyethylenimine
Gene delivery
Laser light scattering
 
Issue Date2011
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel
 
CitationJournal Of Controlled Release, 2011, v. 155 n. 1, p. 67-76 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jconrel.2010.10.028
 
AbstractOur revisit of the complexation between anionic DNA and cationic polyethylenimine (PEI) in both water and phosphate buffered saline (PBS) by using a combination of laser light scattering (LLS) and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~ 3, but the PEI/DNA polyplexes have a high in-vitro gene transfection efficiency only when N:P ≥ 10. Putting these two facts together, we not only conclude that this extra 7 portions of PEI chains are free in the solution mixture, but also confirmed that it is these free PEI chains that substantially promote the gene transfection no matter whether they are applied hours before or after the administration of the much less effective PEI/DNA polyplexes (N:P = 3). The uptake kinetics measured by flow cytometry shows that the addition of free PEI leads to a faster and more efficient cellular internalization of polyplexes, but these free PEI chains mainly contribute to the subsequent intracellular trafficking. In contrast, the bound PEI chains mainly play a role in the DNA condensation and protection, leading to a different thinking in the development of non-viral vectors. © 2010 Elsevier B.V. All rights reserved.
 
ISSN0168-3659
2012 Impact Factor: 7.633
2012 SCImago Journal Rankings: 2.061
 
DOIhttp://dx.doi.org/10.1016/j.jconrel.2010.10.028
 
ISI Accession Number IDWOS:000295998200012
Funding AgencyGrant Number
National Natural Scientific Foundation of China (NNSFC)50773077
20934005
Hong Kong Special Administration Region Earmarked (RGC)CUHK4037/07P
2160331
CUHK4046/08P
2160365
CUHK4039/08P
2160361
CUHK4042/09P
2160396
Funding Information:

The financial support of the National Natural Scientific Foundation of China (NNSFC) Projects (50773077 and 20934005) and the Hong Kong Special Administration Region Earmarked (RGC) Projects (CUHK4037/07P, 2160331; CUHK4046/08P, 2160365; CUHK4039/08P, 2160361; and CUHK4042/09P, 2160396) is gratefully acknowledged.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYue, Y
 
dc.contributor.authorJin, F
 
dc.contributor.authorDeng, R
 
dc.contributor.authorCai, J
 
dc.contributor.authorChen, Y
 
dc.contributor.authorLin, MCM
 
dc.contributor.authorKung, HF
 
dc.contributor.authorWu, C
 
dc.date.accessioned2010-12-23T08:32:44Z
 
dc.date.available2010-12-23T08:32:44Z
 
dc.date.issued2011
 
dc.description.abstractOur revisit of the complexation between anionic DNA and cationic polyethylenimine (PEI) in both water and phosphate buffered saline (PBS) by using a combination of laser light scattering (LLS) and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~ 3, but the PEI/DNA polyplexes have a high in-vitro gene transfection efficiency only when N:P ≥ 10. Putting these two facts together, we not only conclude that this extra 7 portions of PEI chains are free in the solution mixture, but also confirmed that it is these free PEI chains that substantially promote the gene transfection no matter whether they are applied hours before or after the administration of the much less effective PEI/DNA polyplexes (N:P = 3). The uptake kinetics measured by flow cytometry shows that the addition of free PEI leads to a faster and more efficient cellular internalization of polyplexes, but these free PEI chains mainly contribute to the subsequent intracellular trafficking. In contrast, the bound PEI chains mainly play a role in the DNA condensation and protection, leading to a different thinking in the development of non-viral vectors. © 2010 Elsevier B.V. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Controlled Release, 2011, v. 155 n. 1, p. 67-76 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jconrel.2010.10.028
 
dc.identifier.citeulike8215993
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jconrel.2010.10.028
 
dc.identifier.epage76
 
dc.identifier.hkuros183297
 
dc.identifier.isiWOS:000295998200012
Funding AgencyGrant Number
National Natural Scientific Foundation of China (NNSFC)50773077
20934005
Hong Kong Special Administration Region Earmarked (RGC)CUHK4037/07P
2160331
CUHK4046/08P
2160365
CUHK4039/08P
2160361
CUHK4042/09P
2160396
Funding Information:

The financial support of the National Natural Scientific Foundation of China (NNSFC) Projects (50773077 and 20934005) and the Hong Kong Special Administration Region Earmarked (RGC) Projects (CUHK4037/07P, 2160331; CUHK4046/08P, 2160365; CUHK4039/08P, 2160361; and CUHK4042/09P, 2160396) is gratefully acknowledged.

 
dc.identifier.issn0168-3659
2012 Impact Factor: 7.633
2012 SCImago Journal Rankings: 2.061
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid21056067
 
dc.identifier.scopuseid_2-s2.0-79953839673
 
dc.identifier.spage67
 
dc.identifier.urihttp://hdl.handle.net/10722/129117
 
dc.identifier.volume155
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofJournal of Controlled Release
 
dc.relation.referencesReferences in Scopus
 
dc.subjectCellular internalization
 
dc.subjectDNA condensation
 
dc.subjectFree polyethylenimine
 
dc.subjectGene delivery
 
dc.subjectLaser light scattering
 
dc.titleRevisit complexation between DNA and polyethylenimine - Effect of uncomplexed chains free in the solution mixture on gene transfection
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. University of Science and Technology of China
  3. Chinese University of Hong Kong