File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Serological profiling of a Candida albicans protein microarray reveals permanent host-pathogen interplay and stage-specific responses during candidemia

TitleSerological profiling of a Candida albicans protein microarray reveals permanent host-pathogen interplay and stage-specific responses during candidemia
Authors
Issue Date2010
PublisherPublic Library of Science. The Journal's web site is located at http://pathogens.plosjournals.org/perlserv/?request=index-html&issn=1553-7374
Citation
P L o S Pathogens, 2010, v. 6 n. 3, p. e1000827 How to Cite?
AbstractCandida albicans in the immunocompetent host is a benign member of the human microbiota. Though, when host physiology is disrupted, this commensal-host interaction can degenerate and lead to an opportunistic infection. Relatively little is known regarding the dynamics of C. albicans colonization and pathogenesis. We developed a C. albicans cell surface protein microarray to profile the immunoglobulin G response during commensal colonization and candidemia. The antibody response from the sera of patients with candidemia and our negative control groups indicate that the immunocompetent host exists in permanent host-pathogen interplay with commensal C. albicans. This report also identifies cell surface antigens that are specific to different phases (i.e. acute, early and mid convalescence) of candidemia. We identified a set of thirteen cell surface antigens capable of distinguishing acute candidemia from healthy individuals and uninfected hospital patients with commensal colonization. Interestingly, a large proportion of these cell surface antigens are involved in either oxidative stress or drug resistance. In addition, we identified 33 antigenic proteins that are enriched in convalescent sera of the candidemia patients. Intriguingly, we found within this subset an increase in antigens associated with heme-associated iron acquisition. These findings have important implications for the mechanisms of C. albicans colonization as well as the development of systemic infection.
Persistent Identifierhttp://hdl.handle.net/10722/129106
ISSN
2015 Impact Factor: 7.003
2015 SCImago Journal Rankings: 5.185
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Institute of Allergy and Infectious DiseasesP01AI061537
National Institute of General Medical SciencesR01GM/AI55155
Funding Information:

National Institute of Allergy and Infectious Diseases (P01AI061537) and National Institute of General Medical Sciences (R01GM/AI55155). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 

DC FieldValueLanguage
dc.contributor.authorMochon, ABen_US
dc.contributor.authorJin, Yen_US
dc.contributor.authorKayala, MAen_US
dc.contributor.authorWingard, JRen_US
dc.contributor.authorClancy, CJen_US
dc.contributor.authorNguyen, MHen_US
dc.contributor.authorFelgner, Pen_US
dc.contributor.authorBaldi, Pen_US
dc.contributor.authorLiu, Hen_US
dc.date.accessioned2010-12-23T08:32:33Z-
dc.date.available2010-12-23T08:32:33Z-
dc.date.issued2010en_US
dc.identifier.citationP L o S Pathogens, 2010, v. 6 n. 3, p. e1000827en_US
dc.identifier.issn1553-7366-
dc.identifier.urihttp://hdl.handle.net/10722/129106-
dc.description.abstractCandida albicans in the immunocompetent host is a benign member of the human microbiota. Though, when host physiology is disrupted, this commensal-host interaction can degenerate and lead to an opportunistic infection. Relatively little is known regarding the dynamics of C. albicans colonization and pathogenesis. We developed a C. albicans cell surface protein microarray to profile the immunoglobulin G response during commensal colonization and candidemia. The antibody response from the sera of patients with candidemia and our negative control groups indicate that the immunocompetent host exists in permanent host-pathogen interplay with commensal C. albicans. This report also identifies cell surface antigens that are specific to different phases (i.e. acute, early and mid convalescence) of candidemia. We identified a set of thirteen cell surface antigens capable of distinguishing acute candidemia from healthy individuals and uninfected hospital patients with commensal colonization. Interestingly, a large proportion of these cell surface antigens are involved in either oxidative stress or drug resistance. In addition, we identified 33 antigenic proteins that are enriched in convalescent sera of the candidemia patients. Intriguingly, we found within this subset an increase in antigens associated with heme-associated iron acquisition. These findings have important implications for the mechanisms of C. albicans colonization as well as the development of systemic infection.-
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://pathogens.plosjournals.org/perlserv/?request=index-html&issn=1553-7374-
dc.relation.ispartofP L o S Pathogensen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAntibodies, Fungal - blood-
dc.subject.meshCandida albicans - growth and development - immunology-
dc.subject.meshCandidiasis - blood - epidemiology - immunology-
dc.subject.meshHost-Pathogen Interactions - immunology-
dc.subject.meshProtein Array Analysis-
dc.titleSerological profiling of a Candida albicans protein microarray reveals permanent host-pathogen interplay and stage-specific responses during candidemiaen_US
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1553-7366&volume=6&issue=3&spage=e1000827&epage=&date=2010&atitle=Serological+profiling+of+a+Candida+albicans+protein+microarray+reveals+permanent+host-pathogen+interplay+and+stage-specific+responses+during+candidemia-
dc.identifier.emailJin, Y: jinye@hkusua.hku.hk-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.ppat.1000827-
dc.identifier.pmid20361054-
dc.identifier.pmcidPMC2845659-
dc.identifier.scopuseid_2-s2.0-77950420271-
dc.identifier.hkuros176684en_US
dc.identifier.volume6en_US
dc.identifier.issue3-
dc.identifier.spagee1000827en_US
dc.identifier.epagee1000827-
dc.identifier.isiWOS:000277720400035-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats