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Article: Corneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration

TitleCorneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration
Authors
Issue Date2010
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.corneajrnl.com/
Citation
Cornea, 2010, v. 29 n. 3, p. 321-330 How to Cite?
AbstractPurpose: To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation. Methods: We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed. Results: Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect. Conclusions: Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain. Copyright © 2010 by Lippincott Williams & Wilkins.
Persistent Identifierhttp://hdl.handle.net/10722/129096
ISSN
2015 Impact Factor: 1.833
2015 SCImago Journal Rankings: 1.273
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of HealthEY10101
EY001792
EY14048
Research to Prevent Blindness
Funding Information:

Supported by National Institutes of Health grants EY10101 ( D. T. A.), EY001792 ( D. T. A.), and EY14048 (J.H.C.) and an unrestricted departmental grant from Research to Prevent Blindness.

References

 

DC FieldValueLanguage
dc.contributor.authorAzar, DTen_HK
dc.contributor.authorCasanova, FHen_HK
dc.contributor.authorMimura, Ten_HK
dc.contributor.authorJain, Sen_HK
dc.contributor.authorZhou, Zen_HK
dc.contributor.authorHan, KYen_HK
dc.contributor.authorChang, JHen_HK
dc.date.accessioned2010-12-23T08:32:26Z-
dc.date.available2010-12-23T08:32:26Z-
dc.date.issued2010en_HK
dc.identifier.citationCornea, 2010, v. 29 n. 3, p. 321-330en_HK
dc.identifier.issn0277-3740en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129096-
dc.description.abstractPurpose: To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation. Methods: We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed. Results: Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect. Conclusions: Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain. Copyright © 2010 by Lippincott Williams & Wilkins.en_HK
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.corneajrnl.com/en_HK
dc.relation.ispartofCorneaen_HK
dc.subject.meshAngiogenesis Inhibitors - physiologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCattleen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshCell Movementen_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshEndothelium, Vascular - cytologyen_HK
dc.subject.meshEpithelium, Corneal - enzymologyen_HK
dc.subject.meshGenetic Vectorsen_HK
dc.subject.meshGreen Fluorescent Proteins - geneticsen_HK
dc.subject.meshMatrix Metalloproteinase 14 - physiologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Knockouten_HK
dc.subject.meshMutagenesis, Site-Directeden_HK
dc.subject.meshPlasmidsen_HK
dc.subject.meshPulmonary Artery - cytologyen_HK
dc.subject.meshRetroviridae - geneticsen_HK
dc.subject.meshTransfectionen_HK
dc.titleCorneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migrationen_HK
dc.typeArticleen_HK
dc.identifier.emailZhou, Z:zhongjun@hkucc.hku.hken_HK
dc.identifier.authorityZhou, Z=rp00503en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/ICO.0b013e3181b1165den_HK
dc.identifier.pmid20118785-
dc.identifier.scopuseid_2-s2.0-77649164902en_HK
dc.identifier.hkuros178648en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77649164902&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue3en_HK
dc.identifier.spage321en_HK
dc.identifier.epage330en_HK
dc.identifier.isiWOS:000275011900015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridAzar, DT=26643368400en_HK
dc.identifier.scopusauthoridCasanova, FH=7004556831en_HK
dc.identifier.scopusauthoridMimura, T=35242662100en_HK
dc.identifier.scopusauthoridJain, S=35184745300en_HK
dc.identifier.scopusauthoridZhou, Z=8631856300en_HK
dc.identifier.scopusauthoridHan, KY=7402960197en_HK
dc.identifier.scopusauthoridChang, JH=22333289500en_HK

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