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Article: Effect of surface treatments of titanium on amphotericin B-treated Candida albicans persister cells

TitleEffect of surface treatments of titanium on amphotericin B-treated Candida albicans persister cells
Authors
KeywordsAntifungal resistance.
Biofilm
Candida albicans
Surface treatments
Titanium
Issue Date2011
PublisherWiley-Blackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MYC
Citation
Mycoses, 2011, v. 54 n. 3, p. 189-194 How to Cite?
AbstractAlthough persister cells in Candida albicans biofilm may contribute to its increased resistance to antifungal drugs, little information is available on the formation of Candida persister cells on titanium surfaces. The effect of different surface treatments of Ti on persister cells was determined in the present study. Titanium discs were surface-treated by three different methods (Group A - polishing, Group B - sandblasting followed by acid-etching, and Group C - sandblasting alone). Persister cells of two C. albicans strains, namely ATCC 90028 and HK30Aa, in biofilm and planktonic states, were measured as the percentage of colony forming units remaining after 24h incubation with various concentrations of amphotericin B. No persister cells were detected in the planktonic cultures. However, 1.5%, 0.1% and 2.4%C. albicans ATCC 90028 persister cells were detected at an AmB concentration of 64μgml-1 in groups A, B and C, respectively; and 0.3%, 0.2% and 0.6% for groups A, B and C, respectively, for HK30Aa. Group C of C. albicans ATCC 90028 appeared to provide a surface relatively unfavourable for the development of persister cells (P<0.01). Whether these results may have implications on the clinical performance of titanium implants warrants further investigation. © 2009 Blackwell Verlag GmbH.
Persistent Identifierhttp://hdl.handle.net/10722/129072
ISSN
2015 Impact Factor: 2.332
2015 SCImago Journal Rankings: 0.921
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong20004356.90800.08007.400.01
Funding Information:

This study is supported by the University of Hong Kong Faculty Research Fund Grant No. 20004356.90800.08007.400.01. The authors would like to thank Ms. Becky Cheung and Mr. Y. Y. Chui for laboratory and technical assistance, Mr. Shadow Yeung for the statistical analyses, and Dr. Trevor Lane for manuscript editing.

References

 

DC FieldValueLanguage
dc.contributor.authorTsang, CSPen_HK
dc.contributor.authorTang, DYKen_HK
dc.date.accessioned2010-12-23T08:32:05Z-
dc.date.available2010-12-23T08:32:05Z-
dc.date.issued2011en_HK
dc.identifier.citationMycoses, 2011, v. 54 n. 3, p. 189-194en_HK
dc.identifier.issn0933-7407en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129072-
dc.description.abstractAlthough persister cells in Candida albicans biofilm may contribute to its increased resistance to antifungal drugs, little information is available on the formation of Candida persister cells on titanium surfaces. The effect of different surface treatments of Ti on persister cells was determined in the present study. Titanium discs were surface-treated by three different methods (Group A - polishing, Group B - sandblasting followed by acid-etching, and Group C - sandblasting alone). Persister cells of two C. albicans strains, namely ATCC 90028 and HK30Aa, in biofilm and planktonic states, were measured as the percentage of colony forming units remaining after 24h incubation with various concentrations of amphotericin B. No persister cells were detected in the planktonic cultures. However, 1.5%, 0.1% and 2.4%C. albicans ATCC 90028 persister cells were detected at an AmB concentration of 64μgml-1 in groups A, B and C, respectively; and 0.3%, 0.2% and 0.6% for groups A, B and C, respectively, for HK30Aa. Group C of C. albicans ATCC 90028 appeared to provide a surface relatively unfavourable for the development of persister cells (P<0.01). Whether these results may have implications on the clinical performance of titanium implants warrants further investigation. © 2009 Blackwell Verlag GmbH.en_HK
dc.languageengen_US
dc.publisherWiley-Blackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MYCen_HK
dc.relation.ispartofMycosesen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.subjectAntifungal resistance.en_HK
dc.subjectBiofilmen_HK
dc.subjectCandida albicansen_HK
dc.subjectSurface treatmentsen_HK
dc.subjectTitaniumen_HK
dc.titleEffect of surface treatments of titanium on amphotericin B-treated Candida albicans persister cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0933-7407&volume=54&issue=3&spage=189&epage=94&date=2011&atitle=Effect+of+surface+treatments+of+titanium+on+amphotericin+B-treated+Candida+albicans+persister+cells-
dc.identifier.emailTsang, CSP:csptsang@hkucc.hku.hken_HK
dc.identifier.authorityTsang, CSP=rp00026en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1111/j.1439-0507.2009.01790.xen_HK
dc.identifier.pmid19793205-
dc.identifier.scopuseid_2-s2.0-79954467281en_HK
dc.identifier.hkuros177033en_US
dc.identifier.hkuros186537-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79954467281&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue3en_HK
dc.identifier.spage189en_HK
dc.identifier.epage194en_HK
dc.identifier.eissn1439-0507-
dc.identifier.isiWOS:000289466500002-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridTsang, CSP=7202936002en_HK
dc.identifier.scopusauthoridTang, DYK=49461852500en_HK
dc.identifier.citeulike9156350-

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