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Article: Human gingiva is another site of C-reactive protein formation

TitleHuman gingiva is another site of C-reactive protein formation
Authors
Issue Date2010
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
Citation
Journal Of Clinical Periodontology, 2010, v. 37 n. 9, p. 789-796 How to Cite?
Abstract
Objectives: C-reactive protein (CRP) is primarily synthesized in the liver. It is hypothesized that human gingiva per se may produce CRP and its expression could be associated with IL-6. This study elucidated the CRP expression profile in human gingiva and its possible association with IL-6. Materials and Methods: Ninety-four gingival biopsies were collected from 44 subjects with chronic periodontitis and 18 periodontally healthy subjects. CRP protein was detected by immunohistochemistry and Western blotting, while CRP and IL-6 mRNAs were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR. CRP protein expression in the reconstituted human gingival epithelia (RHGE) was examined by the particle-enhanced immunoturbidimetric assay and Western blotting. Results: CRP protein was detected in gingival tissues from patients and healthy subjects by immunohistochemistry and confirmed by Western blotting. Its expression pattern and level at 16 pairs of periodontal pocket tissues and the adjacent clinically healthy tissues from 16 patients were significantly interrelated (rs=0.693, p<0.01). CRP mRNA expression was strongly correlated with IL-6 (r=0.694, p<0.001). Both CRP protein and mRNA were detected in the RHGE. Conclusions: The present study shows for the first time that human gingiva is able to produce CRP in situ that may be associated with IL-6 activity. © 2010 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/129059
ISSN
2013 Impact Factor: 3.610
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
HKU766909M
University of Hong KongCRCG 200807176048
200907176052
Funding Information:

The authors declare that they have no conflict of interests. This study was supported by the Hong Kong Research Grants Council (HKU 7518/05M and HKU766909M) and The University of Hong Kong (CRCG 200807176048 and 200907176052).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLu, Qen_HK
dc.contributor.authorJin, Len_HK
dc.date.accessioned2010-12-23T08:31:59Z-
dc.date.available2010-12-23T08:31:59Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Clinical Periodontology, 2010, v. 37 n. 9, p. 789-796en_HK
dc.identifier.issn0303-6979en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129059-
dc.description.abstractObjectives: C-reactive protein (CRP) is primarily synthesized in the liver. It is hypothesized that human gingiva per se may produce CRP and its expression could be associated with IL-6. This study elucidated the CRP expression profile in human gingiva and its possible association with IL-6. Materials and Methods: Ninety-four gingival biopsies were collected from 44 subjects with chronic periodontitis and 18 periodontally healthy subjects. CRP protein was detected by immunohistochemistry and Western blotting, while CRP and IL-6 mRNAs were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR. CRP protein expression in the reconstituted human gingival epithelia (RHGE) was examined by the particle-enhanced immunoturbidimetric assay and Western blotting. Results: CRP protein was detected in gingival tissues from patients and healthy subjects by immunohistochemistry and confirmed by Western blotting. Its expression pattern and level at 16 pairs of periodontal pocket tissues and the adjacent clinically healthy tissues from 16 patients were significantly interrelated (rs=0.693, p<0.01). CRP mRNA expression was strongly correlated with IL-6 (r=0.694, p<0.001). Both CRP protein and mRNA were detected in the RHGE. Conclusions: The present study shows for the first time that human gingiva is able to produce CRP in situ that may be associated with IL-6 activity. © 2010 John Wiley & Sons A/S.en_HK
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPEen_HK
dc.relation.ispartofJournal of Clinical Periodontologyen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAlveolar Bone Loss - radiographyen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshC-Reactive Protein - analysis - geneticsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshChronic Periodontitis - metabolism - pathologyen_HK
dc.subject.meshConnective Tissue - metabolism - pathologyen_HK
dc.subject.meshEndothelial Cells - metabolism - pathologyen_HK
dc.subject.meshEpithelium - metabolism - pathologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFibroblasts - metabolism - pathologyen_HK
dc.subject.meshGingiva - metabolism - pathologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshInterleukin-6 - analysis - geneticsen_HK
dc.subject.meshKeratinocytes - metabolism - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPeriodontal Attachment Loss - metabolism - pathologyen_HK
dc.subject.meshPeriodontal Pocket - metabolism - pathologyen_HK
dc.subject.meshRNA, Messenger - analysisen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.titleHuman gingiva is another site of C-reactive protein formationen_HK
dc.typeArticleen_HK
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-051X.2010.01600.xen_HK
dc.identifier.pmid20666874en_HK
dc.identifier.scopuseid_2-s2.0-77955761243en_HK
dc.identifier.hkuros178363en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955761243&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue9en_HK
dc.identifier.spage789en_HK
dc.identifier.epage796en_HK
dc.identifier.eissn1600-051X-
dc.identifier.isiWOS:000280991500001-
dc.publisher.placeDenmarken_HK
dc.relation.projectNovel molecular mechanisms of innate host defense - implications in periodontal health and disease-
dc.identifier.scopusauthoridLu, Q=36128876000en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.citeulike7782296-

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