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Article: Human gingiva is another site of C-reactive protein formation
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TitleHuman gingiva is another site of C-reactive protein formation
 
AuthorsLu, Q1
Jin, L1
 
Issue Date2010
 
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
 
CitationJournal Of Clinical Periodontology, 2010, v. 37 n. 9, p. 789-796 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2010.01600.x
 
AbstractObjectives: C-reactive protein (CRP) is primarily synthesized in the liver. It is hypothesized that human gingiva per se may produce CRP and its expression could be associated with IL-6. This study elucidated the CRP expression profile in human gingiva and its possible association with IL-6. Materials and Methods: Ninety-four gingival biopsies were collected from 44 subjects with chronic periodontitis and 18 periodontally healthy subjects. CRP protein was detected by immunohistochemistry and Western blotting, while CRP and IL-6 mRNAs were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR. CRP protein expression in the reconstituted human gingival epithelia (RHGE) was examined by the particle-enhanced immunoturbidimetric assay and Western blotting. Results: CRP protein was detected in gingival tissues from patients and healthy subjects by immunohistochemistry and confirmed by Western blotting. Its expression pattern and level at 16 pairs of periodontal pocket tissues and the adjacent clinically healthy tissues from 16 patients were significantly interrelated (rs=0.693, p<0.01). CRP mRNA expression was strongly correlated with IL-6 (r=0.694, p<0.001). Both CRP protein and mRNA were detected in the RHGE. Conclusions: The present study shows for the first time that human gingiva is able to produce CRP in situ that may be associated with IL-6 activity. © 2010 John Wiley & Sons A/S.
 
ISSN0303-6979
2012 Impact Factor: 3.688
2012 SCImago Journal Rankings: 1.298
 
DOIhttp://dx.doi.org/10.1111/j.1600-051X.2010.01600.x
 
ISI Accession Number IDWOS:000280991500001
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
HKU766909M
University of Hong KongCRCG 200807176048
200907176052
Funding Information:

The authors declare that they have no conflict of interests. This study was supported by the Hong Kong Research Grants Council (HKU 7518/05M and HKU766909M) and The University of Hong Kong (CRCG 200807176048 and 200907176052).

 
ReferencesReferences in Scopus
 
GrantsNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
 
DC FieldValue
dc.contributor.authorLu, Q
 
dc.contributor.authorJin, L
 
dc.date.accessioned2010-12-23T08:31:59Z
 
dc.date.available2010-12-23T08:31:59Z
 
dc.date.issued2010
 
dc.description.abstractObjectives: C-reactive protein (CRP) is primarily synthesized in the liver. It is hypothesized that human gingiva per se may produce CRP and its expression could be associated with IL-6. This study elucidated the CRP expression profile in human gingiva and its possible association with IL-6. Materials and Methods: Ninety-four gingival biopsies were collected from 44 subjects with chronic periodontitis and 18 periodontally healthy subjects. CRP protein was detected by immunohistochemistry and Western blotting, while CRP and IL-6 mRNAs were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR. CRP protein expression in the reconstituted human gingival epithelia (RHGE) was examined by the particle-enhanced immunoturbidimetric assay and Western blotting. Results: CRP protein was detected in gingival tissues from patients and healthy subjects by immunohistochemistry and confirmed by Western blotting. Its expression pattern and level at 16 pairs of periodontal pocket tissues and the adjacent clinically healthy tissues from 16 patients were significantly interrelated (rs=0.693, p<0.01). CRP mRNA expression was strongly correlated with IL-6 (r=0.694, p<0.001). Both CRP protein and mRNA were detected in the RHGE. Conclusions: The present study shows for the first time that human gingiva is able to produce CRP in situ that may be associated with IL-6 activity. © 2010 John Wiley & Sons A/S.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Clinical Periodontology, 2010, v. 37 n. 9, p. 789-796 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2010.01600.x
 
dc.identifier.citeulike7782296
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-051X.2010.01600.x
 
dc.identifier.eissn1600-051X
 
dc.identifier.epage796
 
dc.identifier.hkuros178363
 
dc.identifier.isiWOS:000280991500001
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
HKU766909M
University of Hong KongCRCG 200807176048
200907176052
Funding Information:

The authors declare that they have no conflict of interests. This study was supported by the Hong Kong Research Grants Council (HKU 7518/05M and HKU766909M) and The University of Hong Kong (CRCG 200807176048 and 200907176052).

 
dc.identifier.issn0303-6979
2012 Impact Factor: 3.688
2012 SCImago Journal Rankings: 1.298
 
dc.identifier.issue9
 
dc.identifier.pmid20666874
 
dc.identifier.scopuseid_2-s2.0-77955761243
 
dc.identifier.spage789
 
dc.identifier.urihttp://hdl.handle.net/10722/129059
 
dc.identifier.volume37
 
dc.languageeng
 
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
 
dc.publisher.placeDenmark
 
dc.relation.ispartofJournal of Clinical Periodontology
 
dc.relation.projectNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdolescent
 
dc.subject.meshAdult
 
dc.subject.meshAged
 
dc.subject.meshAlveolar Bone Loss - radiography
 
dc.subject.meshBlotting, Western
 
dc.subject.meshC-Reactive Protein - analysis - genetics
 
dc.subject.meshCells, Cultured
 
dc.subject.meshChronic Periodontitis - metabolism - pathology
 
dc.subject.meshConnective Tissue - metabolism - pathology
 
dc.subject.meshEndothelial Cells - metabolism - pathology
 
dc.subject.meshEpithelium - metabolism - pathology
 
dc.subject.meshFemale
 
dc.subject.meshFibroblasts - metabolism - pathology
 
dc.subject.meshGingiva - metabolism - pathology
 
dc.subject.meshHumans
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshInterleukin-6 - analysis - genetics
 
dc.subject.meshKeratinocytes - metabolism - pathology
 
dc.subject.meshMale
 
dc.subject.meshMiddle Aged
 
dc.subject.meshPeriodontal Attachment Loss - metabolism - pathology
 
dc.subject.meshPeriodontal Pocket - metabolism - pathology
 
dc.subject.meshRNA, Messenger - analysis
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.titleHuman gingiva is another site of C-reactive protein formation
 
dc.typeArticle
 
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Author Affiliations
  1. Prince Philip Dental Hospital