Article: Human gingiva is another site of C-reactive protein formation
| Title | Human gingiva is another site of C-reactive protein formation | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Lu, Q1 Jin, L1 | ||||||
| Issue Date | 2010 | ||||||
| Publisher | Blackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE | ||||||
| Citation | Journal Of Clinical Periodontology, 2010, v. 37 n. 9, p. 789-796 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1600-051X.2010.01600.x | ||||||
| Abstract | Objectives: C-reactive protein (CRP) is primarily synthesized in the liver. It is hypothesized that human gingiva per se may produce CRP and its expression could be associated with IL-6. This study elucidated the CRP expression profile in human gingiva and its possible association with IL-6. Materials and Methods: Ninety-four gingival biopsies were collected from 44 subjects with chronic periodontitis and 18 periodontally healthy subjects. CRP protein was detected by immunohistochemistry and Western blotting, while CRP and IL-6 mRNAs were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR. CRP protein expression in the reconstituted human gingival epithelia (RHGE) was examined by the particle-enhanced immunoturbidimetric assay and Western blotting. Results: CRP protein was detected in gingival tissues from patients and healthy subjects by immunohistochemistry and confirmed by Western blotting. Its expression pattern and level at 16 pairs of periodontal pocket tissues and the adjacent clinically healthy tissues from 16 patients were significantly interrelated (rs=0.693, p<0.01). CRP mRNA expression was strongly correlated with IL-6 (r=0.694, p<0.001). Both CRP protein and mRNA were detected in the RHGE. Conclusions: The present study shows for the first time that human gingiva is able to produce CRP in situ that may be associated with IL-6 activity. © 2010 John Wiley & Sons A/S. | ||||||
| ISSN | 0303-6979 2011 Impact Factor: 2.996 2011 SCImago Journal Rankings: 0.160 | ||||||
| DOI | http://dx.doi.org/10.1111/j.1600-051X.2010.01600.x | ||||||
| ISI Accession Number ID | WOS:000280991500001
Funding Information: The authors declare that they have no conflict of interests. This study was supported by the Hong Kong Research Grants Council (HKU 7518/05M and HKU766909M) and The University of Hong Kong (CRCG 200807176048 and 200907176052). | ||||||
| References | References in Scopus | ||||||
| Grants | A clinical investigation on the association of periodontal disease with endothelial biomarkers and dysfunction Novel molecular mechanisms of innate host defense - implications in periodontal health and disease Novel molecular mechanisms of innate host defense - implications in periodontal health and disease |
| dc.contributor.author | Lu, Q | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Jin, L | ||||||
| dc.date.accessioned | 2010-12-23T08:31:59Z | ||||||
| dc.date.available | 2010-12-23T08:31:59Z | ||||||
| dc.date.issued | 2010 | ||||||
| dc.description.abstract | Objectives: C-reactive protein (CRP) is primarily synthesized in the liver. It is hypothesized that human gingiva per se may produce CRP and its expression could be associated with IL-6. This study elucidated the CRP expression profile in human gingiva and its possible association with IL-6. Materials and Methods: Ninety-four gingival biopsies were collected from 44 subjects with chronic periodontitis and 18 periodontally healthy subjects. CRP protein was detected by immunohistochemistry and Western blotting, while CRP and IL-6 mRNAs were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR. CRP protein expression in the reconstituted human gingival epithelia (RHGE) was examined by the particle-enhanced immunoturbidimetric assay and Western blotting. Results: CRP protein was detected in gingival tissues from patients and healthy subjects by immunohistochemistry and confirmed by Western blotting. Its expression pattern and level at 16 pairs of periodontal pocket tissues and the adjacent clinically healthy tissues from 16 patients were significantly interrelated (rs=0.693, p<0.01). CRP mRNA expression was strongly correlated with IL-6 (r=0.694, p<0.001). Both CRP protein and mRNA were detected in the RHGE. Conclusions: The present study shows for the first time that human gingiva is able to produce CRP in situ that may be associated with IL-6 activity. © 2010 John Wiley & Sons A/S. | ||||||
| dc.description.grant | A clinical investigation on the association of periodontal disease with endothelial biomarkers and dysfunction | ||||||
| dc.description.grant | Novel molecular mechanisms of innate host defense - implications in periodontal health and disease | ||||||
| dc.description.grant | Novel molecular mechanisms of innate host defense - implications in periodontal health and disease | ||||||
| dc.description.grantcode | 101322 | ||||||
| dc.description.grantcode | 28079 | ||||||
| dc.description.grantcode | 29685 | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Journal Of Clinical Periodontology, 2010, v. 37 n. 9, p. 789-796 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1600-051X.2010.01600.x | ||||||
| dc.identifier.citeulike | 7782296 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1111/j.1600-051X.2010.01600.x | ||||||
| dc.identifier.epage | 796 | ||||||
| dc.identifier.hkuros | 178363 | ||||||
| dc.identifier.isi | WOS:000280991500001
Funding Information: The authors declare that they have no conflict of interests. This study was supported by the Hong Kong Research Grants Council (HKU 7518/05M and HKU766909M) and The University of Hong Kong (CRCG 200807176048 and 200907176052). | ||||||
| dc.identifier.issn | 0303-6979 2011 Impact Factor: 2.996 2011 SCImago Journal Rankings: 0.160 | ||||||
| dc.identifier.issue | 9 | ||||||
| dc.identifier.pmid | 20666874 | ||||||
| dc.identifier.scopus | eid_2-s2.0-77955761243 | ||||||
| dc.identifier.spage | 789 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/129059 | ||||||
| dc.identifier.volume | 37 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Blackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE | ||||||
| dc.publisher.place | Denmark | ||||||
| dc.relation.ispartof | Journal of Clinical Periodontology | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Adolescent | ||||||
| dc.subject.mesh | Adult | ||||||
| dc.subject.mesh | Aged | ||||||
| dc.subject.mesh | Alveolar Bone Loss - radiography | ||||||
| dc.subject.mesh | Blotting, Western | ||||||
| dc.subject.mesh | C-Reactive Protein - analysis - genetics | ||||||
| dc.subject.mesh | Cells, Cultured | ||||||
| dc.subject.mesh | Chronic Periodontitis - metabolism - pathology | ||||||
| dc.subject.mesh | Connective Tissue - metabolism - pathology | ||||||
| dc.subject.mesh | Endothelial Cells - metabolism - pathology | ||||||
| dc.subject.mesh | Epithelium - metabolism - pathology | ||||||
| dc.subject.mesh | Female | ||||||
| dc.subject.mesh | Fibroblasts - metabolism - pathology | ||||||
| dc.subject.mesh | Gingiva - metabolism - pathology | ||||||
| dc.subject.mesh | Humans | ||||||
| dc.subject.mesh | Immunohistochemistry | ||||||
| dc.subject.mesh | Interleukin-6 - analysis - genetics | ||||||
| dc.subject.mesh | Keratinocytes - metabolism - pathology | ||||||
| dc.subject.mesh | Male | ||||||
| dc.subject.mesh | Middle Aged | ||||||
| dc.subject.mesh | Periodontal Attachment Loss - metabolism - pathology | ||||||
| dc.subject.mesh | Periodontal Pocket - metabolism - pathology | ||||||
| dc.subject.mesh | RNA, Messenger - analysis | ||||||
| dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | ||||||
| dc.title | Human gingiva is another site of C-reactive protein formation | ||||||
| dc.type | Article |
Author Affiliations
- Prince Philip Dental Hospital