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Article: Growth-differentiation factor-8 (GDF-8) in the uterus: Its identification and functional significance in the golden hamster

TitleGrowth-differentiation factor-8 (GDF-8) in the uterus: Its identification and functional significance in the golden hamster
Authors
Issue Date2009
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.rbej.com/home
Citation
Reproductive Biology And Endocrinology, 2009, v. 7 How to Cite?
AbstractTransforming growth factor-beta superfamily regulates many aspects of reproduction in the female. We identified a novel member of this family, growth-differentiation factor 8 (GDF-8) in the 72 h post coital uterine fluid of the golden hamster by proteomic techniques. Uterine GDF-8 mRNA decreased as pregnancy progressed while its active protein peaked at 72 h post coitus (hpc) and thereafter stayed at a lower level. At 72 hpc, the GDF-8 transcript was localized to the endometrial epithelium while its protein accumulated in the stroma. Exogenous GDF-8 slowed down proliferation of primary cultures of uterine smooth muscle cells (SMC) and endometrial epithelial cells (EEC). In addition, GDF-8 attenuated the release of LIF (leukaemia inhibiting factor) by EEC. As for the embryo in culture, GDF-8 promoted proliferation of the trophotoderm (TM) and hatching but discouraged attachment. Our study suggests that GDF-8 could regulate the behavior of preimplantation embryos and fine-tune the physiology of uterine environment during pregnancy. © 2009 Wong et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/129013
ISSN
2015 Impact Factor: 2.147
2015 SCImago Journal Rankings: 0.919
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council of the Hong Kong Special Administrative RegionCUHK4422/05M
University of Hong Kong10400067.03678.20200.301.01
Funding Information:

This work was supported by a Competitive Earmark Grant (CUHK4422/05M) awarded by the Research Grant Council of the Hong Kong Special Administrative Region and a Small Project Grant (10400067.03678.20200.301.01) from the University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorWong, CLen_HK
dc.contributor.authorHuang, YYen_HK
dc.contributor.authorHo, WKen_HK
dc.contributor.authorPoon, HKen_HK
dc.contributor.authorCheung, PLen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorChow, PHen_HK
dc.date.accessioned2010-12-10T02:13:04Z-
dc.date.available2010-12-10T02:13:04Z-
dc.date.issued2009en_HK
dc.identifier.citationReproductive Biology And Endocrinology, 2009, v. 7en_HK
dc.identifier.issn1477-7827en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129013-
dc.description.abstractTransforming growth factor-beta superfamily regulates many aspects of reproduction in the female. We identified a novel member of this family, growth-differentiation factor 8 (GDF-8) in the 72 h post coital uterine fluid of the golden hamster by proteomic techniques. Uterine GDF-8 mRNA decreased as pregnancy progressed while its active protein peaked at 72 h post coitus (hpc) and thereafter stayed at a lower level. At 72 hpc, the GDF-8 transcript was localized to the endometrial epithelium while its protein accumulated in the stroma. Exogenous GDF-8 slowed down proliferation of primary cultures of uterine smooth muscle cells (SMC) and endometrial epithelial cells (EEC). In addition, GDF-8 attenuated the release of LIF (leukaemia inhibiting factor) by EEC. As for the embryo in culture, GDF-8 promoted proliferation of the trophotoderm (TM) and hatching but discouraged attachment. Our study suggests that GDF-8 could regulate the behavior of preimplantation embryos and fine-tune the physiology of uterine environment during pregnancy. © 2009 Wong et al; licensee BioMed Central Ltd.en_HK
dc.languageeng-
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.rbej.com/homeen_HK
dc.relation.ispartofReproductive Biology and Endocrinologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsReproductive Biology and Endocrinology. Copyright © BioMed Central Ltd.-
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshMesocricetus - genetics - metabolism - physiology-
dc.subject.meshMyostatin - genetics - metabolism - pharmacology - physiology-
dc.subject.meshUterus - metabolism - physiology-
dc.titleGrowth-differentiation factor-8 (GDF-8) in the uterus: Its identification and functional significance in the golden hamsteren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1477-7827&volume=7&issue=134&spage=&epage=&date=2009&atitle=Growth-differentiation+factor-8+(GDF-8)+in+the+uterus:+its+identification+and+functional+significance+in+the+golden+hamster-
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1477-7827-7-134en_HK
dc.identifier.pmid19930721en_HK
dc.identifier.pmcidPMC2790456-
dc.identifier.scopuseid_2-s2.0-71949125322en_HK
dc.identifier.hkuros170745-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-71949125322&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue134-
dc.identifier.isiWOS:000272721400001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, CL=26535204400en_HK
dc.identifier.scopusauthoridHuang, YY=35219961100en_HK
dc.identifier.scopusauthoridHo, WK=34972988300en_HK
dc.identifier.scopusauthoridPoon, HK=7007103075en_HK
dc.identifier.scopusauthoridCheung, PL=35219746800en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK
dc.identifier.citeulike6234963-

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