Granted Patent: Two-Step Enzyme Method for Preparing 7-Aminocephalosporanic Acid
| Title | Two-Step Enzyme Method for Preparing 7-Aminocephalosporanic Acid |
|---|---|
| Granted Patent | US 8003358 |
| Granted Date | 2011-08-23 |
| Priority Date | 2005-08-08 CN20051089965 2006-08-02 PCT/CN2006001940 |
| Inventors | Wang, J Tsang, WK Yap, HK Chen, JM Siu, YL Tsang, SY |
| Issue Date | 2010 |
| Citation | US Patent US 8003358. Washington, DC: US Patent and Trademark Office (USPTO), 2011 [How to Cite?] |
| Abstract | The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeast Trigonopsis variabilis, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, [beta]-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity. |
| References | US 7517677 (B2) 2009-04-14 US 2008009052 (A1) 2008-01-10 CN 1104255 (A) 1995-06-28 CN 1065278 (C) 2001-05-02 CN 1301813 (A) 2001-07-04 CN 1371999 (A) 2002-10-02 CN 1151261 (C) 2004-05-26 CN 1428424 (A) 2003-07-09 |
| dc.contributor.inventor | Wang, J |
|---|---|
| dc.contributor.inventor | Tsang, WK |
| dc.contributor.inventor | Yap, HK |
| dc.contributor.inventor | Chen, JM |
| dc.contributor.inventor | Siu, YL |
| dc.contributor.inventor | Tsang, SY |
| dc.date.accessioned | 2010-10-31T14:07:18Z |
| patents.date.application | 2006-08-02 |
| dc.date.available | 2010-10-31T14:07:18Z |
| patents.date.granted | 2011-08-23 |
| dc.date.issued | 2010 |
| patents.date.priority | 2005-08-08 CN20051089965 |
| patents.date.priority | 2006-08-02 PCT/CN2006001940 |
| dc.description.abstract | The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeast Trigonopsis variabilis, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, [beta]-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity. |
| patents.description.assignee | BIORIGHT WORLDWIDE COMPANY LTD [GB] |
| patents.description.cc | US |
| patents.description.country | United States of America |
| patents.description.kind | B2 |
| patents.identifier.application | US 11/990115 |
| dc.identifier.citation | US Patent US 8003358. Washington, DC: US Patent and Trademark Office (USPTO), 2011 [How to Cite?] |
| patents.identifier.granted | US 8003358 |
| dc.identifier.hkuros | 175329 |
| dc.identifier.uri | http://hdl.handle.net/10722/128140 |
| dc.language | eng |
| patents.relation.family | CN 1912130 (A) 2007-02-14 |
| patents.relation.family | EP 1925663 (A1) 2008-05-28 |
| patents.relation.family | US 2010112633 (A1) 2010-05-06 |
| patents.relation.family | WO 2007016861 (A1) 2007-02-15 |
| dc.relation.references | US 7517677 (B2) 2009-04-14 |
| dc.relation.references | US 2008009052 (A1) 2008-01-10 |
| dc.relation.references | CN 1104255 (A) 1995-06-28 |
| dc.relation.references | CN 1065278 (C) 2001-05-02 |
| dc.relation.references | CN 1301813 (A) 2001-07-04 |
| dc.relation.references | CN 1371999 (A) 2002-10-02 |
| dc.relation.references | CN 1151261 (C) 2004-05-26 |
| dc.relation.references | CN 1428424 (A) 2003-07-09 |
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License For Public Patent Documents |
| dc.title | Two-Step Enzyme Method for Preparing 7-Aminocephalosporanic Acid |
| dc.type | Patent |
| patents.type | Patent_granted |

