Conference Paper: Lycium barbarum polysaccharides protects retina from ischemia/reperfusion injury

TitleLycium barbarum polysaccharides protects retina from ischemia/reperfusion injury
Authors
Keywords685 retina
530 ganglion cells
426 apoptosis/cell death
Issue Date2010
PublisherARVO.
Citation
The 2010 Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO), Fort Lauderdale, FL., 2-6 May 2010. How to Cite?
AbstractPURPOSE: Retinal ischemia/reperfusion (I/R) injury leads to irreversible damage to retinal neurons and structures. It is common in various ocular complications, such as glaucoma, diabetic retinopathy and retinal vessel occlusion. In the present study, we aimed to evaluate the effects of Lycium barbarum polysaccharides (LBP), a traditional Chinese medicine, in a mouse model of retinal I/R injury. METHODS: Adult C57/BL6N mice were orally treated with either vehicle (PBS) or LBP (1 mg/kg or 10 mg/kg once daily) for 1 week before induction of retinal ischemia by the intraluminal suture method. After 2 hours of retinal ischemia followed by 22 hours of reperfusion, retinae were immediately harvested, fixed, and paraffin-embedded for subsequent histological and immunohistochemical analyses. Morphological changes, cell counting and retinal thickness were assessed on hematoxylin and eosin-stained retinal sections. Apoptosis was determined using TUNEL assay and number of apoptotic cells was counted. Blood vessel leakage indicated by IgG extravasations was quantified. Glial fibrillary acidic protein (GFAP) immunohistochemistry was performed. RESULTS: Significantly fewer viable cells were found in the ganglion cell layer (GCL) of vehicle-treated retinae after retinal I/R injury. However, LBP treatment yielded more viable cells and fewer dead cells in GCL. This finding of less neuronal cell death in LBP-treated groups was further confirmed by TUNEL assay where significantly fewer apoptotic cells were identified. In addition, the significant retinal swelling induced by retinal I/R injury in the vehicle-treated group was not observed in LBP-treated groups. Moreover, intense GFAP immunoreactivity and IgG extravasations were observed in vehicle-treated group but not in LBP treated groups. CONCLUSIONS: Our data indicate that pre-treatment with LBP could protect the retina from retinal I/R damage via reducing neuronal cell death, apoptosis, retinal swelling, GFAP activation and blood vessel leakage. These suggest that LBP may be used as a preventive medicine for retinal ischemia.
DescriptionProgram#/Poster#: 4707/D1045
Poster Session: 461 - Ischemia
Persistent Identifierhttp://hdl.handle.net/10722/127950

 

DC FieldValueLanguage
dc.contributor.authorLi, SYen_HK
dc.contributor.authorYeung, CMen_HK
dc.contributor.authorChang, RCCen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorWong, Den_HK
dc.contributor.authorLo, ACYen_HK
dc.date.accessioned2010-10-31T13:56:08Z-
dc.date.available2010-10-31T13:56:08Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 2010 Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO), Fort Lauderdale, FL., 2-6 May 2010.en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127950-
dc.descriptionProgram#/Poster#: 4707/D1045-
dc.descriptionPoster Session: 461 - Ischemia-
dc.description.abstractPURPOSE: Retinal ischemia/reperfusion (I/R) injury leads to irreversible damage to retinal neurons and structures. It is common in various ocular complications, such as glaucoma, diabetic retinopathy and retinal vessel occlusion. In the present study, we aimed to evaluate the effects of Lycium barbarum polysaccharides (LBP), a traditional Chinese medicine, in a mouse model of retinal I/R injury. METHODS: Adult C57/BL6N mice were orally treated with either vehicle (PBS) or LBP (1 mg/kg or 10 mg/kg once daily) for 1 week before induction of retinal ischemia by the intraluminal suture method. After 2 hours of retinal ischemia followed by 22 hours of reperfusion, retinae were immediately harvested, fixed, and paraffin-embedded for subsequent histological and immunohistochemical analyses. Morphological changes, cell counting and retinal thickness were assessed on hematoxylin and eosin-stained retinal sections. Apoptosis was determined using TUNEL assay and number of apoptotic cells was counted. Blood vessel leakage indicated by IgG extravasations was quantified. Glial fibrillary acidic protein (GFAP) immunohistochemistry was performed. RESULTS: Significantly fewer viable cells were found in the ganglion cell layer (GCL) of vehicle-treated retinae after retinal I/R injury. However, LBP treatment yielded more viable cells and fewer dead cells in GCL. This finding of less neuronal cell death in LBP-treated groups was further confirmed by TUNEL assay where significantly fewer apoptotic cells were identified. In addition, the significant retinal swelling induced by retinal I/R injury in the vehicle-treated group was not observed in LBP-treated groups. Moreover, intense GFAP immunoreactivity and IgG extravasations were observed in vehicle-treated group but not in LBP treated groups. CONCLUSIONS: Our data indicate that pre-treatment with LBP could protect the retina from retinal I/R damage via reducing neuronal cell death, apoptosis, retinal swelling, GFAP activation and blood vessel leakage. These suggest that LBP may be used as a preventive medicine for retinal ischemia.-
dc.languageengen_HK
dc.publisherARVO.-
dc.relation.ispartofARVO 2010 Annual Meeting-
dc.subject685 retina-
dc.subject530 ganglion cells-
dc.subject426 apoptosis/cell death-
dc.titleLycium barbarum polysaccharides protects retina from ischemia/reperfusion injuryen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLi, SY: sukyeeli@hku.hken_HK
dc.identifier.emailYeung, CM: ycm1@hku.hken_HK
dc.identifier.emailChang, RCC: rccchang@hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hku.hken_HK
dc.identifier.emailWong, D: shdwong@hku.hken_HK
dc.identifier.emailLo, ACY: amylo@hku.hken_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityWong, D=rp00516en_HK
dc.identifier.authorityLo, ACY=rp00425en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros181390en_HK
dc.publisher.placeUnited States-

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