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Conference Paper: Analysis of a putative VPAC2 receptor from sturgeon shed light on molecular and functional evolution of VPAC2R in vertebrates

TitleAnalysis of a putative VPAC2 receptor from sturgeon shed light on molecular and functional evolution of VPAC2R in vertebrates
Authors
KeywordsMedical sciences
Psychiatry and neurology
Issue Date2009
PublisherHumana Press, Inc.
Citation
The 9th International Symposium on VIP, PACAP and Related Peptides, Kagoshima, Japan, 5-8 October 2009. In Journal of Molecular Neuroscience, 2009, v. 42 n. 3, p. 289 How to Cite?
AbstractVasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) are structurally related neuropeptides that exert multiple physiological effects in our body. Their actions are mediated through the activation of three receptors: PAC1R, VPAC1R, and VPAC2R. Despite the importance of these intercellular communicators, there is little information available for PACAP, VIP, and their receptors in extant early vertebrate species. In this study, a full-length VPAC2R cDNA was identified from a sturgeon species, Acipenser schrenskii. This cDNA is 1,498 bp in length encoding for a protein with 427 amino acids. Phylogenetic analysis showed that the sturgeon VPAC2R is structurally more similar to tetrapod VPAC2Rs than teleost VPAC2Rs or PHIRs. By real-time PCR, it was found that the sturgeon VPAC2R was widely distributed in various tissues, with the highest expression in gut and with moderate expression in liver and gonad. In functional cAMP assays, the sturgeon VPAC2R could be stimulated by mammalian and fish VIPs but not human and fish PHIs and PACAPs. These data suggested that the ability of VPAC2R to interact with PACAP evolved after the teleost/ tetrapod split in the tetrapod lineage. Moreover, the previously isolated fish PHIRs which are structurally related to VPAC2Rs in vertebrates may exist only in teleosts via the teleost-specific 3R genome duplication.
DescriptionSession 7: Receptor and Pharmacology. No. O19
Persistent Identifierhttp://hdl.handle.net/10722/127811
ISSN
2015 Impact Factor: 2.352
2015 SCImago Journal Rankings: 0.988

 

DC FieldValueLanguage
dc.contributor.authorLee, TOen_HK
dc.contributor.authorTam, JKVen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-10-31T13:47:54Z-
dc.date.available2010-10-31T13:47:54Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 9th International Symposium on VIP, PACAP and Related Peptides, Kagoshima, Japan, 5-8 October 2009. In Journal of Molecular Neuroscience, 2009, v. 42 n. 3, p. 289en_HK
dc.identifier.issn0895-8696-
dc.identifier.urihttp://hdl.handle.net/10722/127811-
dc.descriptionSession 7: Receptor and Pharmacology. No. O19-
dc.description.abstractVasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) are structurally related neuropeptides that exert multiple physiological effects in our body. Their actions are mediated through the activation of three receptors: PAC1R, VPAC1R, and VPAC2R. Despite the importance of these intercellular communicators, there is little information available for PACAP, VIP, and their receptors in extant early vertebrate species. In this study, a full-length VPAC2R cDNA was identified from a sturgeon species, Acipenser schrenskii. This cDNA is 1,498 bp in length encoding for a protein with 427 amino acids. Phylogenetic analysis showed that the sturgeon VPAC2R is structurally more similar to tetrapod VPAC2Rs than teleost VPAC2Rs or PHIRs. By real-time PCR, it was found that the sturgeon VPAC2R was widely distributed in various tissues, with the highest expression in gut and with moderate expression in liver and gonad. In functional cAMP assays, the sturgeon VPAC2R could be stimulated by mammalian and fish VIPs but not human and fish PHIs and PACAPs. These data suggested that the ability of VPAC2R to interact with PACAP evolved after the teleost/ tetrapod split in the tetrapod lineage. Moreover, the previously isolated fish PHIRs which are structurally related to VPAC2Rs in vertebrates may exist only in teleosts via the teleost-specific 3R genome duplication.-
dc.languageengen_HK
dc.publisherHumana Press, Inc.-
dc.relation.ispartofJournal of Molecular Neuroscience-
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectMedical sciences-
dc.subjectPsychiatry and neurology-
dc.titleAnalysis of a putative VPAC2 receptor from sturgeon shed light on molecular and functional evolution of VPAC2R in vertebratesen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0895-8696&volume=42&issue=3&spage=289&epage=&date=2009&atitle=Analysis+of+a+putative+VPAC2+receptor+from+sturgeon+shed+light+on+molecular+and+functional+evolution+of+VPAC2R+in+vertebrates-
dc.identifier.emailLee, TO: ltolee2@hkucc.hku.hken_HK
dc.identifier.emailTam, JKV: janicekalvan@gmail.comen_HK
dc.identifier.emailChow, BKC: bkcc@hkusua.hku.hken_HK
dc.identifier.hkuros176306en_HK
dc.identifier.volume42-
dc.identifier.issue3-
dc.identifier.spage289-
dc.identifier.epage289-
dc.description.otherThe 9th International Symposium on VIP, PACAP and Related Peptides, Kagoshima, Japan, 5-8 October 2009. In Journal of Molecular Neuroscience, 2009, v. 42 n. 3, p. 289-

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