Article: Evaluation of the adjuvant properties of Astragalus membranaceus and Scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models
File Download
(May Require Subscription)
- No File Attached
(May Require Subscription)
- Publisher Website: 10.1016/j.vaccine.2009.10.065
- Scopus: eid_2-s2.0-71149088939
- PMID: 19887128
- Find via
Supplementary
-
Citations:
-
Appears in Collections:
| Title | Evaluation of the adjuvant properties of Astragalus membranaceus and Scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Authors | Yang, X1 Huang, S1 Chen, J1 Song, N1 Wang, L1 Zhang, Z1 Deng, G1 Zheng, H1 Zhu, XQ1 Lu, F1 | ||||||||||
| Keywords | Astragalus membranaceus Mice Scutellaria baicalensis UV-attenuated T. gondii | ||||||||||
| Issue Date | 2010 | ||||||||||
| Publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine | ||||||||||
| Citation | Vaccine, 2010, v. 28 n. 3, p. 737-743 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.vaccine.2009.10.065 | ||||||||||
| Abstract | Human vaccines are not available and current anti-toxoplasma treatment is disappointing. To investigate the possible adjuvant effect of aqueous extracts obtained from medicinal herbs of Astragalus membranaceus (Am) and Scutellaria baicalensis GEORGI (Sb) on the immune response to Toxoplasma gondii in the mouse models induced by ultraviolet (UV)-attenuated T. gondii, this paper studies the possible vaccination strategies to help combat infections with Toxoplasma and looking towards developing new vaccine and approaches. We used UV-attenuated T. gondii (UV-T.g) of RH strain as a vaccine and the extracts of Am (AmE) and Sb (SbE) as adjuvant. Mice were infected by intraperitoneal (i.p.) injection of 102 RH tachyzoites alone (infected controls), infected and treated with AmE (T.g + AmE) and SbE (T.g + SbE), respectively; and mice immunized i.p. with UV-T.g alone, UV-T.g co-administrated with AmE (UV-T.g + AmE) or SbE (UV-T.g + SbE), and then challenged with T.g, respectively. The animal survival time, parasite burden in peritoneal lavage fluids, liver histopathological analysis, and levels of serum antibodies among the groups were compared after either infection or challenge. The results showed that, compared to infected controls, infected mice treated with AmE or SbE, or vaccinated mice and then challenged, had significantly prolonged survival time, decreased parasite burden, improved liver histopathological score, and increased Th1-type cellular immune response; furthermore, vaccinated mice co-administrated with AmE or SbE had even longer survival, lower parasite burden, lower liver histopathological score, and higher Th1 response after challenge. Our data demonstrated that the protective immunity of UV-attenuated T. gondii could be markedly enhanced by AmE or SbE co-administration, which suggests that both AmE and SbE may have the potential to be used as effective vaccine adjuvant. © 2009 Elsevier Ltd. All rights reserved. | ||||||||||
| ISSN | 0264-410X 2011 Impact Factor: 3.766 2011 SCImago Journal Rankings: 0.369 | ||||||||||
| DOI | http://dx.doi.org/10.1016/j.vaccine.2009.10.065 | ||||||||||
| ISI Accession Number ID | WOS:000275015200020
Funding Information: This work was supported, in part, by grants from the National Basic Research Program of China (973 Program) (No. 2010CB530001), the Guangdong Provincial Natural Science Foundation of China to F.L. (06021302), the National Special Research Program for Non-Profit Trades (Agriculture) to X.Q.Z. (No. 200803017), and the Program for Changjiang Scholars and innovative Research Team in University to X.Q.Z. (IRT0723). | ||||||||||
| References | References in Scopus |
| dc.contributor.author | Yang, X | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Huang, S | ||||||||||
| dc.contributor.author | Chen, J | ||||||||||
| dc.contributor.author | Song, N | ||||||||||
| dc.contributor.author | Wang, L | ||||||||||
| dc.contributor.author | Zhang, Z | ||||||||||
| dc.contributor.author | Deng, G | ||||||||||
| dc.contributor.author | Zheng, H | ||||||||||
| dc.contributor.author | Zhu, XQ | ||||||||||
| dc.contributor.author | Lu, F | ||||||||||
| dc.date.accessioned | 2010-10-31T13:35:26Z | ||||||||||
| dc.date.available | 2010-10-31T13:35:26Z | ||||||||||
| dc.date.issued | 2010 | ||||||||||
| dc.description.abstract | Human vaccines are not available and current anti-toxoplasma treatment is disappointing. To investigate the possible adjuvant effect of aqueous extracts obtained from medicinal herbs of Astragalus membranaceus (Am) and Scutellaria baicalensis GEORGI (Sb) on the immune response to Toxoplasma gondii in the mouse models induced by ultraviolet (UV)-attenuated T. gondii, this paper studies the possible vaccination strategies to help combat infections with Toxoplasma and looking towards developing new vaccine and approaches. We used UV-attenuated T. gondii (UV-T.g) of RH strain as a vaccine and the extracts of Am (AmE) and Sb (SbE) as adjuvant. Mice were infected by intraperitoneal (i.p.) injection of 102 RH tachyzoites alone (infected controls), infected and treated with AmE (T.g + AmE) and SbE (T.g + SbE), respectively; and mice immunized i.p. with UV-T.g alone, UV-T.g co-administrated with AmE (UV-T.g + AmE) or SbE (UV-T.g + SbE), and then challenged with T.g, respectively. The animal survival time, parasite burden in peritoneal lavage fluids, liver histopathological analysis, and levels of serum antibodies among the groups were compared after either infection or challenge. The results showed that, compared to infected controls, infected mice treated with AmE or SbE, or vaccinated mice and then challenged, had significantly prolonged survival time, decreased parasite burden, improved liver histopathological score, and increased Th1-type cellular immune response; furthermore, vaccinated mice co-administrated with AmE or SbE had even longer survival, lower parasite burden, lower liver histopathological score, and higher Th1 response after challenge. Our data demonstrated that the protective immunity of UV-attenuated T. gondii could be markedly enhanced by AmE or SbE co-administration, which suggests that both AmE and SbE may have the potential to be used as effective vaccine adjuvant. © 2009 Elsevier Ltd. All rights reserved. | ||||||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||||||
| dc.identifier.citation | Vaccine, 2010, v. 28 n. 3, p. 737-743 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.vaccine.2009.10.065 | ||||||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.vaccine.2009.10.065 | ||||||||||
| dc.identifier.epage | 743 | ||||||||||
| dc.identifier.hkuros | 173786 | ||||||||||
| dc.identifier.isi | WOS:000275015200020
Funding Information: This work was supported, in part, by grants from the National Basic Research Program of China (973 Program) (No. 2010CB530001), the Guangdong Provincial Natural Science Foundation of China to F.L. (06021302), the National Special Research Program for Non-Profit Trades (Agriculture) to X.Q.Z. (No. 200803017), and the Program for Changjiang Scholars and innovative Research Team in University to X.Q.Z. (IRT0723). | ||||||||||
| dc.identifier.issn | 0264-410X 2011 Impact Factor: 3.766 2011 SCImago Journal Rankings: 0.369 | ||||||||||
| dc.identifier.issue | 3 | ||||||||||
| dc.identifier.openurl | | ||||||||||
| dc.identifier.pmid | 19887128 | ||||||||||
| dc.identifier.scopus | eid_2-s2.0-71149088939 | ||||||||||
| dc.identifier.spage | 737 | ||||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/127609 | ||||||||||
| dc.identifier.volume | 28 | ||||||||||
| dc.language | eng | ||||||||||
| dc.publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine | ||||||||||
| dc.publisher.place | United Kingdom | ||||||||||
| dc.relation.ispartof | Vaccine | ||||||||||
| dc.relation.references | References in Scopus | ||||||||||
| dc.subject.mesh | Adjuvants, Immunologic - administration and dosage - isolation and purification - pharmacology | ||||||||||
| dc.subject.mesh | Astragalus membranaceus - chemistry | ||||||||||
| dc.subject.mesh | Plant Extracts - administration and dosage - isolation and purification - pharmacology | ||||||||||
| dc.subject.mesh | Protozoan Vaccines - immunology | ||||||||||
| dc.subject.mesh | Scutellaria baicalensis - chemistry | ||||||||||
| dc.subject | Astragalus membranaceus | ||||||||||
| dc.subject | Mice | ||||||||||
| dc.subject | Scutellaria baicalensis | ||||||||||
| dc.subject | UV-attenuated T. gondii | ||||||||||
| dc.title | Evaluation of the adjuvant properties of Astragalus membranaceus and Scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models | ||||||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong