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Article: Evaluation of the adjuvant properties of Astragalus membranaceus and Scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models

TitleEvaluation of the adjuvant properties of Astragalus membranaceus and Scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models
Authors
KeywordsAstragalus membranaceus
Mice
Scutellaria baicalensis
UV-attenuated T. gondii
Issue Date2010
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
Citation
Vaccine, 2010, v. 28 n. 3, p. 737-743 How to Cite?
Abstract
Human vaccines are not available and current anti-toxoplasma treatment is disappointing. To investigate the possible adjuvant effect of aqueous extracts obtained from medicinal herbs of Astragalus membranaceus (Am) and Scutellaria baicalensis GEORGI (Sb) on the immune response to Toxoplasma gondii in the mouse models induced by ultraviolet (UV)-attenuated T. gondii, this paper studies the possible vaccination strategies to help combat infections with Toxoplasma and looking towards developing new vaccine and approaches. We used UV-attenuated T. gondii (UV-T.g) of RH strain as a vaccine and the extracts of Am (AmE) and Sb (SbE) as adjuvant. Mice were infected by intraperitoneal (i.p.) injection of 102 RH tachyzoites alone (infected controls), infected and treated with AmE (T.g + AmE) and SbE (T.g + SbE), respectively; and mice immunized i.p. with UV-T.g alone, UV-T.g co-administrated with AmE (UV-T.g + AmE) or SbE (UV-T.g + SbE), and then challenged with T.g, respectively. The animal survival time, parasite burden in peritoneal lavage fluids, liver histopathological analysis, and levels of serum antibodies among the groups were compared after either infection or challenge. The results showed that, compared to infected controls, infected mice treated with AmE or SbE, or vaccinated mice and then challenged, had significantly prolonged survival time, decreased parasite burden, improved liver histopathological score, and increased Th1-type cellular immune response; furthermore, vaccinated mice co-administrated with AmE or SbE had even longer survival, lower parasite burden, lower liver histopathological score, and higher Th1 response after challenge. Our data demonstrated that the protective immunity of UV-attenuated T. gondii could be markedly enhanced by AmE or SbE co-administration, which suggests that both AmE and SbE may have the potential to be used as effective vaccine adjuvant. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/127609
ISSN
2013 Impact Factor: 3.485
ISI Accession Number ID
Funding AgencyGrant Number
National Basic Research Program of China2010CB530001
Guangdong Provincial Natural Science Foundation of China06021302
National Special Research Program200803017
Program for Changjiang Scholars and innovative Research Team in UniversityIRT0723
Funding Information:

This work was supported, in part, by grants from the National Basic Research Program of China (973 Program) (No. 2010CB530001), the Guangdong Provincial Natural Science Foundation of China to F.L. (06021302), the National Special Research Program for Non-Profit Trades (Agriculture) to X.Q.Z. (No. 200803017), and the Program for Changjiang Scholars and innovative Research Team in University to X.Q.Z. (IRT0723).

References

 

DC FieldValueLanguage
dc.contributor.authorYang, Xen_HK
dc.contributor.authorHuang, Sen_HK
dc.contributor.authorChen, Jen_HK
dc.contributor.authorSong, Nen_HK
dc.contributor.authorWang, Len_HK
dc.contributor.authorZhang, Zen_HK
dc.contributor.authorDeng, Gen_HK
dc.contributor.authorZheng, Hen_HK
dc.contributor.authorZhu, XQen_HK
dc.contributor.authorLu, Fen_HK
dc.date.accessioned2010-10-31T13:35:26Z-
dc.date.available2010-10-31T13:35:26Z-
dc.date.issued2010en_HK
dc.identifier.citationVaccine, 2010, v. 28 n. 3, p. 737-743en_HK
dc.identifier.issn0264-410Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/127609-
dc.description.abstractHuman vaccines are not available and current anti-toxoplasma treatment is disappointing. To investigate the possible adjuvant effect of aqueous extracts obtained from medicinal herbs of Astragalus membranaceus (Am) and Scutellaria baicalensis GEORGI (Sb) on the immune response to Toxoplasma gondii in the mouse models induced by ultraviolet (UV)-attenuated T. gondii, this paper studies the possible vaccination strategies to help combat infections with Toxoplasma and looking towards developing new vaccine and approaches. We used UV-attenuated T. gondii (UV-T.g) of RH strain as a vaccine and the extracts of Am (AmE) and Sb (SbE) as adjuvant. Mice were infected by intraperitoneal (i.p.) injection of 102 RH tachyzoites alone (infected controls), infected and treated with AmE (T.g + AmE) and SbE (T.g + SbE), respectively; and mice immunized i.p. with UV-T.g alone, UV-T.g co-administrated with AmE (UV-T.g + AmE) or SbE (UV-T.g + SbE), and then challenged with T.g, respectively. The animal survival time, parasite burden in peritoneal lavage fluids, liver histopathological analysis, and levels of serum antibodies among the groups were compared after either infection or challenge. The results showed that, compared to infected controls, infected mice treated with AmE or SbE, or vaccinated mice and then challenged, had significantly prolonged survival time, decreased parasite burden, improved liver histopathological score, and increased Th1-type cellular immune response; furthermore, vaccinated mice co-administrated with AmE or SbE had even longer survival, lower parasite burden, lower liver histopathological score, and higher Th1 response after challenge. Our data demonstrated that the protective immunity of UV-attenuated T. gondii could be markedly enhanced by AmE or SbE co-administration, which suggests that both AmE and SbE may have the potential to be used as effective vaccine adjuvant. © 2009 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccineen_HK
dc.relation.ispartofVaccineen_HK
dc.subjectAstragalus membranaceusen_HK
dc.subjectMiceen_HK
dc.subjectScutellaria baicalensisen_HK
dc.subjectUV-attenuated T. gondiien_HK
dc.subject.meshAdjuvants, Immunologic - administration and dosage - isolation and purification - pharmacology-
dc.subject.meshAstragalus membranaceus - chemistry-
dc.subject.meshPlant Extracts - administration and dosage - isolation and purification - pharmacology-
dc.subject.meshProtozoan Vaccines - immunology-
dc.subject.meshScutellaria baicalensis - chemistry-
dc.titleEvaluation of the adjuvant properties of Astragalus membranaceus and Scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated Toxoplasma gondii in mouse modelsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0264-410X&volume=28&issue=3&spage=737&epage=743&date=2010&atitle=Evaluation+of+the+adjuvant+properties+of+Astragalus+membranaceus+and+Scutellaria+baicalensis+GEORGI+in+the+immune+protection+induced+by+UV-attenuated+Toxoplasma+gondii+in+mouse+modelsen_HK
dc.identifier.emailChen, J: abchen@hku.hken_HK
dc.identifier.authorityChen, J=rp01316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.vaccine.2009.10.065en_HK
dc.identifier.pmid19887128en_HK
dc.identifier.scopuseid_2-s2.0-71149088939en_HK
dc.identifier.hkuros173786en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-71149088939&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue3en_HK
dc.identifier.spage737en_HK
dc.identifier.epage743en_HK
dc.identifier.isiWOS:000275015200020-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYang, X=22952286800en_HK
dc.identifier.scopusauthoridHuang, S=7405422703en_HK
dc.identifier.scopusauthoridChen, J=22733695400en_HK
dc.identifier.scopusauthoridSong, N=35489195800en_HK
dc.identifier.scopusauthoridWang, L=35099881800en_HK
dc.identifier.scopusauthoridZhang, Z=35489706400en_HK
dc.identifier.scopusauthoridDeng, G=35486921400en_HK
dc.identifier.scopusauthoridZheng, H=7403440550en_HK
dc.identifier.scopusauthoridZhu, XQ=8393069200en_HK
dc.identifier.scopusauthoridLu, F=7402968086en_HK

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