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Article: Evaluation of the adjuvant properties of Astragalus membranaceus and Scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models
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TitleEvaluation of the adjuvant properties of Astragalus membranaceus and Scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models
 
AuthorsYang, X3
Huang, S1
Chen, J2
Song, N1
Wang, L3
Zhang, Z1
Deng, G1
Zheng, H3
Zhu, XQ4
Lu, F3
 
KeywordsAstragalus membranaceus
Mice
Scutellaria baicalensis
UV-attenuated T. gondii
 
Issue Date2010
 
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
 
CitationVaccine, 2010, v. 28 n. 3, p. 737-743 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.vaccine.2009.10.065
 
AbstractHuman vaccines are not available and current anti-toxoplasma treatment is disappointing. To investigate the possible adjuvant effect of aqueous extracts obtained from medicinal herbs of Astragalus membranaceus (Am) and Scutellaria baicalensis GEORGI (Sb) on the immune response to Toxoplasma gondii in the mouse models induced by ultraviolet (UV)-attenuated T. gondii, this paper studies the possible vaccination strategies to help combat infections with Toxoplasma and looking towards developing new vaccine and approaches. We used UV-attenuated T. gondii (UV-T.g) of RH strain as a vaccine and the extracts of Am (AmE) and Sb (SbE) as adjuvant. Mice were infected by intraperitoneal (i.p.) injection of 102 RH tachyzoites alone (infected controls), infected and treated with AmE (T.g + AmE) and SbE (T.g + SbE), respectively; and mice immunized i.p. with UV-T.g alone, UV-T.g co-administrated with AmE (UV-T.g + AmE) or SbE (UV-T.g + SbE), and then challenged with T.g, respectively. The animal survival time, parasite burden in peritoneal lavage fluids, liver histopathological analysis, and levels of serum antibodies among the groups were compared after either infection or challenge. The results showed that, compared to infected controls, infected mice treated with AmE or SbE, or vaccinated mice and then challenged, had significantly prolonged survival time, decreased parasite burden, improved liver histopathological score, and increased Th1-type cellular immune response; furthermore, vaccinated mice co-administrated with AmE or SbE had even longer survival, lower parasite burden, lower liver histopathological score, and higher Th1 response after challenge. Our data demonstrated that the protective immunity of UV-attenuated T. gondii could be markedly enhanced by AmE or SbE co-administration, which suggests that both AmE and SbE may have the potential to be used as effective vaccine adjuvant. © 2009 Elsevier Ltd. All rights reserved.
 
ISSN0264-410X
2013 Impact Factor: 3.485
 
DOIhttp://dx.doi.org/10.1016/j.vaccine.2009.10.065
 
ISI Accession Number IDWOS:000275015200020
Funding AgencyGrant Number
National Basic Research Program of China2010CB530001
Guangdong Provincial Natural Science Foundation of China06021302
National Special Research Program200803017
Program for Changjiang Scholars and innovative Research Team in UniversityIRT0723
Funding Information:

This work was supported, in part, by grants from the National Basic Research Program of China (973 Program) (No. 2010CB530001), the Guangdong Provincial Natural Science Foundation of China to F.L. (06021302), the National Special Research Program for Non-Profit Trades (Agriculture) to X.Q.Z. (No. 200803017), and the Program for Changjiang Scholars and innovative Research Team in University to X.Q.Z. (IRT0723).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYang, X
 
dc.contributor.authorHuang, S
 
dc.contributor.authorChen, J
 
dc.contributor.authorSong, N
 
dc.contributor.authorWang, L
 
dc.contributor.authorZhang, Z
 
dc.contributor.authorDeng, G
 
dc.contributor.authorZheng, H
 
dc.contributor.authorZhu, XQ
 
dc.contributor.authorLu, F
 
dc.date.accessioned2010-10-31T13:35:26Z
 
dc.date.available2010-10-31T13:35:26Z
 
dc.date.issued2010
 
dc.description.abstractHuman vaccines are not available and current anti-toxoplasma treatment is disappointing. To investigate the possible adjuvant effect of aqueous extracts obtained from medicinal herbs of Astragalus membranaceus (Am) and Scutellaria baicalensis GEORGI (Sb) on the immune response to Toxoplasma gondii in the mouse models induced by ultraviolet (UV)-attenuated T. gondii, this paper studies the possible vaccination strategies to help combat infections with Toxoplasma and looking towards developing new vaccine and approaches. We used UV-attenuated T. gondii (UV-T.g) of RH strain as a vaccine and the extracts of Am (AmE) and Sb (SbE) as adjuvant. Mice were infected by intraperitoneal (i.p.) injection of 102 RH tachyzoites alone (infected controls), infected and treated with AmE (T.g + AmE) and SbE (T.g + SbE), respectively; and mice immunized i.p. with UV-T.g alone, UV-T.g co-administrated with AmE (UV-T.g + AmE) or SbE (UV-T.g + SbE), and then challenged with T.g, respectively. The animal survival time, parasite burden in peritoneal lavage fluids, liver histopathological analysis, and levels of serum antibodies among the groups were compared after either infection or challenge. The results showed that, compared to infected controls, infected mice treated with AmE or SbE, or vaccinated mice and then challenged, had significantly prolonged survival time, decreased parasite burden, improved liver histopathological score, and increased Th1-type cellular immune response; furthermore, vaccinated mice co-administrated with AmE or SbE had even longer survival, lower parasite burden, lower liver histopathological score, and higher Th1 response after challenge. Our data demonstrated that the protective immunity of UV-attenuated T. gondii could be markedly enhanced by AmE or SbE co-administration, which suggests that both AmE and SbE may have the potential to be used as effective vaccine adjuvant. © 2009 Elsevier Ltd. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationVaccine, 2010, v. 28 n. 3, p. 737-743 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.vaccine.2009.10.065
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.vaccine.2009.10.065
 
dc.identifier.epage743
 
dc.identifier.hkuros173786
 
dc.identifier.isiWOS:000275015200020
Funding AgencyGrant Number
National Basic Research Program of China2010CB530001
Guangdong Provincial Natural Science Foundation of China06021302
National Special Research Program200803017
Program for Changjiang Scholars and innovative Research Team in UniversityIRT0723
Funding Information:

This work was supported, in part, by grants from the National Basic Research Program of China (973 Program) (No. 2010CB530001), the Guangdong Provincial Natural Science Foundation of China to F.L. (06021302), the National Special Research Program for Non-Profit Trades (Agriculture) to X.Q.Z. (No. 200803017), and the Program for Changjiang Scholars and innovative Research Team in University to X.Q.Z. (IRT0723).

 
dc.identifier.issn0264-410X
2013 Impact Factor: 3.485
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid19887128
 
dc.identifier.scopuseid_2-s2.0-71149088939
 
dc.identifier.spage737
 
dc.identifier.urihttp://hdl.handle.net/10722/127609
 
dc.identifier.volume28
 
dc.languageeng
 
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofVaccine
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdjuvants, Immunologic - administration and dosage - isolation and purification - pharmacology
 
dc.subject.meshAstragalus membranaceus - chemistry
 
dc.subject.meshPlant Extracts - administration and dosage - isolation and purification - pharmacology
 
dc.subject.meshProtozoan Vaccines - immunology
 
dc.subject.meshScutellaria baicalensis - chemistry
 
dc.subjectAstragalus membranaceus
 
dc.subjectMice
 
dc.subjectScutellaria baicalensis
 
dc.subjectUV-attenuated T. gondii
 
dc.titleEvaluation of the adjuvant properties of Astragalus membranaceus and Scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated Toxoplasma gondii in mouse models
 
dc.typeArticle
 
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Author Affiliations
  1. Jinan University, School of Medicine
  2. The University of Hong Kong
  3. Sun Yat-Sen University
  4. South China Agricultural University