File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Tian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo

TitleTian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo
Authors
KeywordsMedical sciences
Issue Date2010
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/home
Citation
Chinese Medicine, 2010, v. 5 How to Cite?
AbstractBackground: Tian Xian Liquid (TXL) is a Chinese medicine decoction and has been used as an anticancer dietary supplement. The present study aims to investigate the effects of TXL on the apoptosis of HT-29 cells and tumor growth in vivo.Method: HT-29 colon cancer cells were treated with gradient dilution of TXL. The mitochondrial membrane potential was measured by JC-1 assay. The release of cytochrome c from mitochondrial and apoptosis-related proteins Bax, Bcl-2, cleaved caspase-3, 9 were examined by Western blot analysis. HT-29 cells were implanted in nude mice to examine the effects of TXL on tumor growth.Result: TXL inhibited HT-29 xenografted model and showed a strong and dose-dependent inhibitory effect on the proliferation of HT-29 cells. Mitochondrial membrane potential was reduced by TXL at the concentration of 0.5% above. For Western blot analysis, an increase in Bax expression and a decrease in Bcl-2 expression were observed in TXL-treated cells. TXL treatment increased the protein level of cleaved casepase-3 and caspase-9, and the release of cytochrome c in cytoplasm was up-regulated as well.Conclusion: TXL significantly inhibits cell proliferation in the HT-29 cells and HT-29 xenografted model via the mitochondrial cell death pathway. © 2010 Liu et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/127605
ISSN
2015 Impact Factor: 1.58
2015 SCImago Journal Rankings: 0.655
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Qen_HK
dc.contributor.authorTong, Yen_HK
dc.contributor.authorSze, SCWen_HK
dc.contributor.authorLiu, WKen_HK
dc.contributor.authorLam, Len_HK
dc.contributor.authorChu, ESMen_HK
dc.contributor.authorYow, CMNen_HK
dc.date.accessioned2010-10-31T13:35:13Z-
dc.date.available2010-10-31T13:35:13Z-
dc.date.issued2010en_HK
dc.identifier.citationChinese Medicine, 2010, v. 5en_HK
dc.identifier.issn1749-8546en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127605-
dc.description.abstractBackground: Tian Xian Liquid (TXL) is a Chinese medicine decoction and has been used as an anticancer dietary supplement. The present study aims to investigate the effects of TXL on the apoptosis of HT-29 cells and tumor growth in vivo.Method: HT-29 colon cancer cells were treated with gradient dilution of TXL. The mitochondrial membrane potential was measured by JC-1 assay. The release of cytochrome c from mitochondrial and apoptosis-related proteins Bax, Bcl-2, cleaved caspase-3, 9 were examined by Western blot analysis. HT-29 cells were implanted in nude mice to examine the effects of TXL on tumor growth.Result: TXL inhibited HT-29 xenografted model and showed a strong and dose-dependent inhibitory effect on the proliferation of HT-29 cells. Mitochondrial membrane potential was reduced by TXL at the concentration of 0.5% above. For Western blot analysis, an increase in Bax expression and a decrease in Bcl-2 expression were observed in TXL-treated cells. TXL treatment increased the protein level of cleaved casepase-3 and caspase-9, and the release of cytochrome c in cytoplasm was up-regulated as well.Conclusion: TXL significantly inhibits cell proliferation in the HT-29 cells and HT-29 xenografted model via the mitochondrial cell death pathway. © 2010 Liu et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/homeen_HK
dc.relation.ispartofChinese Medicineen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsChinese Medicine. Copyright © BioMed Central Ltd.-
dc.subjectMedical sciences-
dc.titleTian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivoen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1749-8546&volume=5&issue=25&spage=&epage=&date=2010&atitle=Tian+Xian+Liquid+(TXL)+induces+apoptosis+in+HT-29+colon+cancer+cell+in+vitro+and+inhibits+tumor+growth+in+vivo.en_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.emailSze, SCW: stephens@hku.hken_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.identifier.authoritySze, SCW=rp00514en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1749-8546-5-25en_HK
dc.identifier.pmid20663169-
dc.identifier.pmcidPMC2918619-
dc.identifier.scopuseid_2-s2.0-77954669821en_HK
dc.identifier.hkuros194477en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954669821&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue25-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLiu, Q=36063708000en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK
dc.identifier.scopusauthoridSze, SCW=23482617000en_HK
dc.identifier.scopusauthoridLiu, WK=37045953300en_HK
dc.identifier.scopusauthoridLam, L=36337060500en_HK
dc.identifier.scopusauthoridChu, ESM=13807807000en_HK
dc.identifier.scopusauthoridYow, CMN=6602535418en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats