Article: Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry

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TitleIdentification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry
AuthorsHu, YM1
Wang, YT2
Sze, SCW1
Tsang, KW1
Wong, HK1
Liu, Q1
Zhong, LD1
Tong, Y1
KeywordsErxian Decoction (EXD)
LC-DAD-ESI-MS
Menopausal syndromes
Metabolites
Pharmacokinetic study
Traditional Chinese medicine prescriptions (TCMP)
Issue Date2010
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/
CitationBiomedical Chromatography, 2010, v. 24 n. 5, p. 479-489 [How to Cite?]
DOI: http://dx.doi.org/10.1002/bmc.1315
AbstractA simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MS n analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MS n fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN0269-3879
2011 Impact Factor: 1.966
2011 SCImago Journal Rankings: 0.110
DOIhttp://dx.doi.org/10.1002/bmc.1315
ISI Accession Number IDWOS:000277335200005
Funding AgencyGrant Number
Small Project Funding200807176144
200607176131
Seed Funding Programme for Applied Research200802160025
University of Hong Kong
Liu Hao Tsing Foundation Ltd
Funding Information:

This research was supported in part by a grant from Small Project Funding (200807176144; 200607176131), Seed Funding Programme for Applied Research (no. 200802160025), the University of Hong Kong and Liu Hao Tsing Foundation Ltd. The authors would also like to thank the Physical & Chemical Testing Center at the Jinan University, Guangzhou, for their assistance in NMR and ESIMS experiments and the Nongben' Company in Hong Kong for supplying the EXD samples.

ReferencesReferences in Scopus
GrantsQuality Assessment of Chinese Medicine Formula, Erxian Decoction, by high-performance liquid chromatography coupled with diode array detector
Mechanistics Study on the Molecular Actions of a Modern Chinese Medicine Decoction (GFP) in Estrogen Secretion in Rat Granulose Cell via Aromatase Activation in vitro.
Bioactive Components of Erxian Decoction as a Potential Drug Candidate for Peri-menopausal Syndrome
DC Field
Value
dc.contributor.authorHu, YM
dc.contributor.authorWang, YT
dc.contributor.authorSze, SCW
dc.contributor.authorTsang, KW
dc.contributor.authorWong, HK
dc.contributor.authorLiu, Q
dc.contributor.authorZhong, LD
dc.contributor.authorTong, Y
dc.date.accessioned2010-10-31T13:35:06Z
dc.date.available2010-10-31T13:35:06Z
dc.date.issued2010
dc.description.abstractA simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MS n analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MS n fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction. Copyright © 2009 John Wiley & Sons, Ltd.
dc.description.grantQuality Assessment of Chinese Medicine Formula, Erxian Decoction, by high-performance liquid chromatography coupled with diode array detector
dc.description.grantMechanistics Study on the Molecular Actions of a Modern Chinese Medicine Decoction (GFP) in Estrogen Secretion in Rat Granulose Cell via Aromatase Activation in vitro.
dc.description.grantBioactive Components of Erxian Decoction as a Potential Drug Candidate for Peri-menopausal Syndrome
dc.description.grantcode99295
dc.description.grantcode82569
dc.description.grantcode98291
dc.description.naturepostprint
dc.identifier.citationBiomedical Chromatography, 2010, v. 24 n. 5, p. 479-489 [How to Cite?]
DOI: http://dx.doi.org/10.1002/bmc.1315
dc.identifier.doihttp://dx.doi.org/10.1002/bmc.1315
dc.identifier.epage489
dc.identifier.hkuros181697
dc.identifier.isiWOS:000277335200005
Funding AgencyGrant Number
Small Project Funding200807176144
200607176131
Seed Funding Programme for Applied Research200802160025
University of Hong Kong
Liu Hao Tsing Foundation Ltd
Funding Information:

This research was supported in part by a grant from Small Project Funding (200807176144; 200607176131), Seed Funding Programme for Applied Research (no. 200802160025), the University of Hong Kong and Liu Hao Tsing Foundation Ltd. The authors would also like to thank the Physical & Chemical Testing Center at the Jinan University, Guangzhou, for their assistance in NMR and ESIMS experiments and the Nongben' Company in Hong Kong for supplying the EXD samples.

dc.identifier.issn0269-3879
2011 Impact Factor: 1.966
2011 SCImago Journal Rankings: 0.110
dc.identifier.issue5
dc.identifier.openurl
dc.identifier.pmid19688819
dc.identifier.scopuseid_2-s2.0-77951763374
dc.identifier.spage479
dc.identifier.urihttp://hdl.handle.net/10722/127603
dc.identifier.volume24
dc.languageeng
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofBiomedical Chromatography
dc.relation.referencesReferences in Scopus
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.rightsBiomedical Chromatography. Copyright © John Wiley & Sons Ltd.
dc.rightsThis is a preprint of an article published in Biomedical Chromatography, 2010, v. 24 n. 5, p. 479-489
dc.subject.meshLC-DAD-ESI-MS
dc.subject.meshErxian Decoction (EXD)
dc.subject.meshTraditional Chinese medicine prescriptions (TCMP)
dc.subject.meshMenopausal syndromes
dc.subject.meshPharmacokinetic study
dc.subjectErxian Decoction (EXD)
dc.subjectLC-DAD-ESI-MS
dc.subjectMenopausal syndromes
dc.subjectMetabolites
dc.subjectPharmacokinetic study
dc.subjectTraditional Chinese medicine prescriptions (TCMP)
dc.titleIdentification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. University of Macau