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Article: Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry
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TitleIdentification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry
 
AuthorsHu, YM1
Wang, YT2
Sze, SCW1
Tsang, KW1
Wong, HK1
Liu, Q1
Zhong, LD1
Tong, Y1
 
KeywordsErxian Decoction (EXD)
LC-DAD-ESI-MS
Menopausal syndromes
Metabolites
Pharmacokinetic study
Traditional Chinese medicine prescriptions (TCMP)
 
Issue Date2010
 
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/
 
CitationBiomedical Chromatography, 2010, v. 24 n. 5, p. 479-489 [How to Cite?]
DOI: http://dx.doi.org/10.1002/bmc.1315
 
AbstractA simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MS n analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MS n fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction. Copyright © 2009 John Wiley & Sons, Ltd.
 
ISSN0269-3879
2012 Impact Factor: 1.945
2012 SCImago Journal Rankings: 0.667
 
DOIhttp://dx.doi.org/10.1002/bmc.1315
 
ISI Accession Number IDWOS:000277335200005
Funding AgencyGrant Number
Small Project Funding200807176144
200607176131
Seed Funding Programme for Applied Research200802160025
University of Hong Kong
Liu Hao Tsing Foundation Ltd
Funding Information:

This research was supported in part by a grant from Small Project Funding (200807176144; 200607176131), Seed Funding Programme for Applied Research (no. 200802160025), the University of Hong Kong and Liu Hao Tsing Foundation Ltd. The authors would also like to thank the Physical & Chemical Testing Center at the Jinan University, Guangzhou, for their assistance in NMR and ESIMS experiments and the Nongben' Company in Hong Kong for supplying the EXD samples.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHu, YM
 
dc.contributor.authorWang, YT
 
dc.contributor.authorSze, SCW
 
dc.contributor.authorTsang, KW
 
dc.contributor.authorWong, HK
 
dc.contributor.authorLiu, Q
 
dc.contributor.authorZhong, LD
 
dc.contributor.authorTong, Y
 
dc.date.accessioned2010-10-31T13:35:06Z
 
dc.date.available2010-10-31T13:35:06Z
 
dc.date.issued2010
 
dc.description.abstractA simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MS n analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MS n fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction. Copyright © 2009 John Wiley & Sons, Ltd.
 
dc.description.naturepostprint
 
dc.identifier.citationBiomedical Chromatography, 2010, v. 24 n. 5, p. 479-489 [How to Cite?]
DOI: http://dx.doi.org/10.1002/bmc.1315
 
dc.identifier.doihttp://dx.doi.org/10.1002/bmc.1315
 
dc.identifier.epage489
 
dc.identifier.hkuros181697
 
dc.identifier.hkuros160701
 
dc.identifier.isiWOS:000277335200005
Funding AgencyGrant Number
Small Project Funding200807176144
200607176131
Seed Funding Programme for Applied Research200802160025
University of Hong Kong
Liu Hao Tsing Foundation Ltd
Funding Information:

This research was supported in part by a grant from Small Project Funding (200807176144; 200607176131), Seed Funding Programme for Applied Research (no. 200802160025), the University of Hong Kong and Liu Hao Tsing Foundation Ltd. The authors would also like to thank the Physical & Chemical Testing Center at the Jinan University, Guangzhou, for their assistance in NMR and ESIMS experiments and the Nongben' Company in Hong Kong for supplying the EXD samples.

 
dc.identifier.issn0269-3879
2012 Impact Factor: 1.945
2012 SCImago Journal Rankings: 0.667
 
dc.identifier.issue5
 
dc.identifier.openurl
 
dc.identifier.pmid19688819
 
dc.identifier.scopuseid_2-s2.0-77951763374
 
dc.identifier.spage479
 
dc.identifier.urihttp://hdl.handle.net/10722/127603
 
dc.identifier.volume24
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofBiomedical Chromatography
 
dc.relation.referencesReferences in Scopus
 
dc.rightsBiomedical Chromatography. Copyright © John Wiley & Sons Ltd.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.rightsThis is a preprint of an article published in Biomedical Chromatography, 2010, v. 24 n. 5, p. 479-489
 
dc.subject.meshLC-DAD-ESI-MS
 
dc.subject.meshErxian Decoction (EXD)
 
dc.subject.meshTraditional Chinese medicine prescriptions (TCMP)
 
dc.subject.meshMenopausal syndromes
 
dc.subject.meshPharmacokinetic study
 
dc.subjectErxian Decoction (EXD)
 
dc.subjectLC-DAD-ESI-MS
 
dc.subjectMenopausal syndromes
 
dc.subjectMetabolites
 
dc.subjectPharmacokinetic study
 
dc.subjectTraditional Chinese medicine prescriptions (TCMP)
 
dc.titleIdentification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. University of Macau