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Article: Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry

TitleIdentification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry
Authors
KeywordsErxian Decoction (EXD)
LC-DAD-ESI-MS
Menopausal syndromes
Metabolites
Pharmacokinetic study
Traditional Chinese medicine prescriptions (TCMP)
Issue Date2010
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/
Citation
Biomedical Chromatography, 2010, v. 24 n. 5, p. 479-489 How to Cite?
Abstract
A simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MS n analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MS n fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction. Copyright © 2009 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/127603
ISSN
2013 Impact Factor: 1.662
ISI Accession Number ID
Funding AgencyGrant Number
Small Project Funding200807176144
200607176131
Seed Funding Programme for Applied Research200802160025
University of Hong Kong
Liu Hao Tsing Foundation Ltd
Funding Information:

This research was supported in part by a grant from Small Project Funding (200807176144; 200607176131), Seed Funding Programme for Applied Research (no. 200802160025), the University of Hong Kong and Liu Hao Tsing Foundation Ltd. The authors would also like to thank the Physical & Chemical Testing Center at the Jinan University, Guangzhou, for their assistance in NMR and ESIMS experiments and the Nongben' Company in Hong Kong for supplying the EXD samples.

References

 

Author Affiliations
  1. The University of Hong Kong
  2. University of Macau
DC FieldValueLanguage
dc.contributor.authorHu, YMen_HK
dc.contributor.authorWang, YTen_HK
dc.contributor.authorSze, SCWen_HK
dc.contributor.authorTsang, KWen_HK
dc.contributor.authorWong, HKen_HK
dc.contributor.authorLiu, Qen_HK
dc.contributor.authorZhong, LDen_HK
dc.contributor.authorTong, Yen_HK
dc.date.accessioned2010-10-31T13:35:06Z-
dc.date.available2010-10-31T13:35:06Z-
dc.date.issued2010en_HK
dc.identifier.citationBiomedical Chromatography, 2010, v. 24 n. 5, p. 479-489en_HK
dc.identifier.issn0269-3879en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127603-
dc.description.abstractA simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MS n analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MS n fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the eff ective EXD fraction. Copyright © 2009 John Wiley & Sons, Ltd.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/en_HK
dc.relation.ispartofBiomedical Chromatographyen_HK
dc.rightsBiomedical Chromatography. Copyright © John Wiley & Sons Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a preprint of an article published in Biomedical Chromatography, 2010, v. 24 n. 5, p. 479-489-
dc.subjectErxian Decoction (EXD)en_HK
dc.subjectLC-DAD-ESI-MSen_HK
dc.subjectMenopausal syndromesen_HK
dc.subjectMetabolitesen_HK
dc.subjectPharmacokinetic studyen_HK
dc.subjectTraditional Chinese medicine prescriptions (TCMP)en_HK
dc.subject.meshLC-DAD-ESI-MS-
dc.subject.meshErxian Decoction (EXD)-
dc.subject.meshTraditional Chinese medicine prescriptions (TCMP)-
dc.subject.meshMenopausal syndromes-
dc.subject.meshPharmacokinetic study-
dc.titleIdentification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0269-3879&volume=24&issue=5&spage=479&epage=489&date=2010&atitle=Identification+of+the+major+chemical+constituents+and+their+metabolites+in+rat+plasma+and+various+organs+after+oral+administration+of+effective+Erxian+Decoction+(EXD)+fraction+by+liquid+chromatography-mass+spectrometryen_HK
dc.identifier.emailSze, SCW: stephens@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.authoritySze, SCW=rp00514en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1002/bmc.1315en_HK
dc.identifier.pmid19688819en_HK
dc.identifier.scopuseid_2-s2.0-77951763374en_HK
dc.identifier.hkuros181697en_HK
dc.identifier.hkuros160701-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77951763374&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue5en_HK
dc.identifier.spage479en_HK
dc.identifier.epage489en_HK
dc.identifier.isiWOS:000277335200005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHu, YM=7407116890en_HK
dc.identifier.scopusauthoridWang, YT=8327361900en_HK
dc.identifier.scopusauthoridSze, SCW=23482617000en_HK
dc.identifier.scopusauthoridTsang, KW=47861536800en_HK
dc.identifier.scopusauthoridWong, HK=36920239700en_HK
dc.identifier.scopusauthoridLiu, Q=36063708000en_HK
dc.identifier.scopusauthoridZhong, LD=36191429900en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK

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