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Article: Proteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HepG2

TitleProteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HepG2
Authors
KeywordsAstragaloside IV
Chemoprevention
Gene regulation
Oncogene Vav3
Tumorigenesis
Issue Date2010
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 2010, v. 36 n. 3, p. 725-735 How to Cite?
AbstractTriterpenoids are implicated in the chemoprevention of various cancers. Current challenge is to define the molecular mechanism underlying the chemopreventive activity of triterpenoids. This study was designed to characterize the intracellular proteins regulated by astragaloside IV, the major active triterpenoid in Radix Astragali. Upon the treatment with astragaloside IV, human hepatocellular carcinoma HepG2 cells were evaluated for the colonogenic survival and anchorage-independent growth. The cellular proteins of treated and untreated cells were resolved by 2-D polyacrylamide gel electrophoresis. The protein spots mostly altered by drug treatment were identified by mass spectrometry and subsequently verified by Western blotting using specific antibodies and RT-PCR technique using specific DNA primers. We found that astragaloside IV attenuated the colonogenic survival and anchorage-independent growth of cancer cells. Based on the proteomic profiles, top 14 upregulated and 13 down-regulated protein spots were subjected to mass spectrometric analysis. As an example, Vav3.1 belongs to the oncogene Vav family, which is implicated in tumorigenesis. Vav3.1 expression was down-regulated by astragaloside IV in a dose- and time-dependent manner. Down-regulation of Vav3.1 was highly correlated with the suppression of cell malignant transform. Thus, astragaloside IV may elicit anticancer activity via down-regulating the expression of oncogenes such as Vav3.1.
Persistent Identifierhttp://hdl.handle.net/10722/127602
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270
ISI Accession Number ID
Funding AgencyGrant Number
Dean's Fund for Traditional Chinese Medicine Program
Li Ka Shing Faculty of Medicine
Seed Funding for Basic Research, The University of Hong Kong
Funding Information:

We acknowledge Miss Yim-Hing Cheung for her excellent technical assistance. We also thank Dr Priscilla Leung and Mr Lawrence Luk from Genome Research Centre, University of Hong Kong, for their technical assistance with 2D SDSPAGE analysis and protein identification by MALDI/TOF mass spectrometry. This work was supported by the Dean's Fund for Traditional Chinese Medicine Program, Li Ka Shing Faculty of Medicine, and the Seed Funding for Basic Research, The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorQi, Hen_HK
dc.contributor.authorWei, Len_HK
dc.contributor.authorHan, Yen_HK
dc.contributor.authorZhang, Qen_HK
dc.contributor.authorLau, ASYen_HK
dc.contributor.authorRong, Jen_HK
dc.date.accessioned2010-10-31T13:35:03Z-
dc.date.available2010-10-31T13:35:03Z-
dc.date.issued2010en_HK
dc.identifier.citationInternational Journal Of Oncology, 2010, v. 36 n. 3, p. 725-735en_HK
dc.identifier.issn1019-6439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127602-
dc.description.abstractTriterpenoids are implicated in the chemoprevention of various cancers. Current challenge is to define the molecular mechanism underlying the chemopreventive activity of triterpenoids. This study was designed to characterize the intracellular proteins regulated by astragaloside IV, the major active triterpenoid in Radix Astragali. Upon the treatment with astragaloside IV, human hepatocellular carcinoma HepG2 cells were evaluated for the colonogenic survival and anchorage-independent growth. The cellular proteins of treated and untreated cells were resolved by 2-D polyacrylamide gel electrophoresis. The protein spots mostly altered by drug treatment were identified by mass spectrometry and subsequently verified by Western blotting using specific antibodies and RT-PCR technique using specific DNA primers. We found that astragaloside IV attenuated the colonogenic survival and anchorage-independent growth of cancer cells. Based on the proteomic profiles, top 14 upregulated and 13 down-regulated protein spots were subjected to mass spectrometric analysis. As an example, Vav3.1 belongs to the oncogene Vav family, which is implicated in tumorigenesis. Vav3.1 expression was down-regulated by astragaloside IV in a dose- and time-dependent manner. Down-regulation of Vav3.1 was highly correlated with the suppression of cell malignant transform. Thus, astragaloside IV may elicit anticancer activity via down-regulating the expression of oncogenes such as Vav3.1.en_HK
dc.languageengen_HK
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_HK
dc.relation.ispartofInternational Journal of Oncologyen_HK
dc.subjectAstragaloside IVen_HK
dc.subjectChemopreventionen_HK
dc.subjectGene regulationen_HK
dc.subjectOncogene Vav3en_HK
dc.subjectTumorigenesisen_HK
dc.subject.meshCarcinoma, Hepatocellular - drug therapy-
dc.subject.meshProteomics - methods-
dc.subject.meshSaponins - pharmacology-
dc.subject.meshTriterpenes - pharmacology-
dc.subject.meshTriterpenes - pharmacology-
dc.titleProteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HepG2en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1019-6439&volume=36&issue=3&spage=725&epage=735&date=2010&atitle=Proteomic+characterization+of+the+cellular+response+to+chemopreventive+triterpenoid+astragaloside+IV+in+human+hepatocelluar+carcinoma+cell+line+HepG2-
dc.identifier.emailLau, ASY: asylau@hku.hken_HK
dc.identifier.emailRong, J: jrong@hku.hken_HK
dc.identifier.authorityLau, ASY=rp00474en_HK
dc.identifier.authorityRong, J=rp00515en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3892/ijo-00000548en_HK
dc.identifier.pmid20126993-
dc.identifier.scopuseid_2-s2.0-77149121747en_HK
dc.identifier.hkuros179325en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77149121747&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue3en_HK
dc.identifier.spage725en_HK
dc.identifier.epage735en_HK
dc.identifier.isiWOS:000274718400023-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridQi, H=35367105300en_HK
dc.identifier.scopusauthoridWei, L=43861929300en_HK
dc.identifier.scopusauthoridHan, Y=8527680500en_HK
dc.identifier.scopusauthoridZhang, Q=12345014000en_HK
dc.identifier.scopusauthoridLau, ASY=7202626202en_HK
dc.identifier.scopusauthoridRong, J=7005980047en_HK

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