File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification of the bioactive constituent and its mechanisms of action in mediating the anti-inflammatory effects of black cohosh and related Cimicifuga species on human primary blood macrophages

TitleIdentification of the bioactive constituent and its mechanisms of action in mediating the anti-inflammatory effects of black cohosh and related Cimicifuga species on human primary blood macrophages
Authors
Issue Date2009
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/jmc
Citation
Journal Of Medicinal Chemistry, 2009, v. 52 n. 21, p. 6707-6715 How to Cite?
AbstractCimicifuga species have been used as traditional medicinal herbs to treat inflammation and symptoms associated with menopause in Asia, Europe, and North America. However, the underlying mechanism of their anti-inflammatory effects remains to be investigated. With bioactivity guided purification involving the use of partitioning extraction and high performance liquid chromatography, we isolated one of the key bioactive constituents from the rhizome extracts of Cimicifuga racemosa. By NMR spectroscopy, the molecule was identified to be cimiracemate A (1). This compound (140 μM) suppressed the lipopolysaccharide-induced TNF-R production in the blood macrophages by 47 (19% and 58 (30% at LPS concentrations of 1 ng/mL and 10 ng/mL, respectively. The antiinflammatory activity of compound 1 may be due to its modulation of a signaling mitogen activated protein kinase and transcription factor nuclear factor-kappaB activities. Compound 1 was found in other Cimicifuga species. Our data indicate that compound 1 or its chemical analogues may have the potential to be further developed as a new class of therapeutic agent. © 2009 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/127585
ISSN
2015 Impact Factor: 5.589
2015 SCImago Journal Rankings: 2.529
ISI Accession Number ID
Funding AgencyGrant Number
Prof Francis SK Lau and Mr. William Au Research Fund
Purapharm International
Funding Information:

This project was supported in part by grants from Prof Francis SK Lau and Mr. William Au Research Fund as well as Purapharm International awarded to Dr. A. Lau. We thank Genome Research Centre of The University of Hong Kong for facility support. We also thank Prof. PY Qian from The University of Science and Technology for providing UPLC-TOF-ESI-MS facilities for spectroscopic analysis and Miss S. Dash for her time and assistance in this regard. Both SCCC and JCBL contributed equally to this manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorYang, CLHen_HK
dc.contributor.authorChik, SCCen_HK
dc.contributor.authorLi, JCBen_HK
dc.contributor.authorCheung, BKWen_HK
dc.contributor.authorLau, ASYen_HK
dc.date.accessioned2010-10-31T13:34:06Z-
dc.date.available2010-10-31T13:34:06Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Medicinal Chemistry, 2009, v. 52 n. 21, p. 6707-6715en_HK
dc.identifier.issn0022-2623en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127585-
dc.description.abstractCimicifuga species have been used as traditional medicinal herbs to treat inflammation and symptoms associated with menopause in Asia, Europe, and North America. However, the underlying mechanism of their anti-inflammatory effects remains to be investigated. With bioactivity guided purification involving the use of partitioning extraction and high performance liquid chromatography, we isolated one of the key bioactive constituents from the rhizome extracts of Cimicifuga racemosa. By NMR spectroscopy, the molecule was identified to be cimiracemate A (1). This compound (140 μM) suppressed the lipopolysaccharide-induced TNF-R production in the blood macrophages by 47 (19% and 58 (30% at LPS concentrations of 1 ng/mL and 10 ng/mL, respectively. The antiinflammatory activity of compound 1 may be due to its modulation of a signaling mitogen activated protein kinase and transcription factor nuclear factor-kappaB activities. Compound 1 was found in other Cimicifuga species. Our data indicate that compound 1 or its chemical analogues may have the potential to be further developed as a new class of therapeutic agent. © 2009 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/jmcen_HK
dc.relation.ispartofJournal of Medicinal Chemistryen_HK
dc.titleIdentification of the bioactive constituent and its mechanisms of action in mediating the anti-inflammatory effects of black cohosh and related Cimicifuga species on human primary blood macrophagesen_HK
dc.typeArticleen_HK
dc.identifier.emailLi, JCB: jamesli@hku.hken_HK
dc.identifier.emailLau, ASY: asylau@hku.hken_HK
dc.identifier.authorityLi, JCB=rp00496en_HK
dc.identifier.authorityLau, ASY=rp00474en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/jm9006164en_HK
dc.identifier.pmid19835377-
dc.identifier.scopuseid_2-s2.0-71049149246en_HK
dc.identifier.hkuros171678en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-71049149246&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue21en_HK
dc.identifier.spage6707en_HK
dc.identifier.epage6715en_HK
dc.identifier.eissn1520-4804-
dc.identifier.isiWOS:000271427900023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYang, CLH=26668171500en_HK
dc.identifier.scopusauthoridChik, SCC=6507803546en_HK
dc.identifier.scopusauthoridLi, JCB=23103447500en_HK
dc.identifier.scopusauthoridCheung, BKW=9634391200en_HK
dc.identifier.scopusauthoridLau, ASY=7202626202en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats