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Article: Synthetic fluorescent probes for imaging of peroxynitrite and hypochlorous acid in living cells.

TitleSynthetic fluorescent probes for imaging of peroxynitrite and hypochlorous acid in living cells.
Authors
Issue Date2010
Citation
Methods In Molecular Biology (Clifton, N.J.), 2010, v. 591, p. 93-103 How to Cite?
AbstractPeroxynitrite (ONOO(-)) and hypochlorous acid (HOCl) are two highly reactive oxygen species generated in biological systems. The overproduction of peroxynitrite or hypochlorous acid is implicated in a broad array of human pathologies including vascular, immunological, and neurodegenerative diseases. However, unambiguous detection of these reactive oxygen species has been relatively difficult due to their short biological half-lives and multiple reaction pathways. Based on their specific chemical reactions, we have developed fluorescent probes HKGreen-1 and HKOCl-1 for highly sensitive detection of peroxynitrite and hypochlorous acid, respectively. Both probes have been demonstrated to be able to discriminate corresponding reactive species from other reactive oxygen and nitrogen species (ROS and RNS) in not only chemical systems but also biological systems. The endogenous production of peroxynitrite in neuronal cells under oxygen-glucose deprivation (OGD) conditions has been visualized for the first time by utilizing HKGreen-1 probe, whilst the endogenous production of hypochlorous acid in macrophage cells upon stimulation with LPS, IFN-gamma, and PMA has been imaged by utilizing HKOCl-1 probe.
Persistent Identifierhttp://hdl.handle.net/10722/127580
ISSN

 

DC FieldValueLanguage
dc.contributor.authorYang, Den_HK
dc.contributor.authorSun, ZNen_HK
dc.contributor.authorPeng, Ten_HK
dc.contributor.authorWang, HLen_HK
dc.contributor.authorShen, JGen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorTam, PKen_HK
dc.date.accessioned2010-10-31T13:33:48Z-
dc.date.available2010-10-31T13:33:48Z-
dc.date.issued2010en_HK
dc.identifier.citationMethods In Molecular Biology (Clifton, N.J.), 2010, v. 591, p. 93-103en_HK
dc.identifier.issn1940-6029en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127580-
dc.description.abstractPeroxynitrite (ONOO(-)) and hypochlorous acid (HOCl) are two highly reactive oxygen species generated in biological systems. The overproduction of peroxynitrite or hypochlorous acid is implicated in a broad array of human pathologies including vascular, immunological, and neurodegenerative diseases. However, unambiguous detection of these reactive oxygen species has been relatively difficult due to their short biological half-lives and multiple reaction pathways. Based on their specific chemical reactions, we have developed fluorescent probes HKGreen-1 and HKOCl-1 for highly sensitive detection of peroxynitrite and hypochlorous acid, respectively. Both probes have been demonstrated to be able to discriminate corresponding reactive species from other reactive oxygen and nitrogen species (ROS and RNS) in not only chemical systems but also biological systems. The endogenous production of peroxynitrite in neuronal cells under oxygen-glucose deprivation (OGD) conditions has been visualized for the first time by utilizing HKGreen-1 probe, whilst the endogenous production of hypochlorous acid in macrophage cells upon stimulation with LPS, IFN-gamma, and PMA has been imaged by utilizing HKOCl-1 probe.en_HK
dc.languageengen_HK
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)en_HK
dc.titleSynthetic fluorescent probes for imaging of peroxynitrite and hypochlorous acid in living cells.en_HK
dc.typeArticleen_HK
dc.identifier.emailYang, D: yangdan@hku.hken_HK
dc.identifier.emailSun, ZN: znsun@hku.hken_HK
dc.identifier.emailShen, JG: shenjg@hku.hken_HK
dc.identifier.emailChen, Y: ychenc@hku.hken_HK
dc.identifier.emailTam, PK: paultam@hku.hken_HK
dc.identifier.authorityYang, D=rp00825en_HK
dc.identifier.authoritySun, ZN=rp00782en_HK
dc.identifier.authorityShen, JG=rp00487en_HK
dc.identifier.authorityChen, Y=rp01318en_HK
dc.identifier.authorityTam, PK=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/978-1-60761-404-3_5-
dc.identifier.pmid19957125-
dc.identifier.scopuseid_2-s2.0-77449087908en_HK
dc.identifier.hkuros176041en_HK
dc.identifier.volume591en_HK
dc.identifier.spage93en_HK
dc.identifier.epage103en_HK
dc.identifier.scopusauthoridYang, D=7404800756en_HK
dc.identifier.scopusauthoridSun, ZN=25029021500en_HK
dc.identifier.scopusauthoridPeng, T=35491190200en_HK
dc.identifier.scopusauthoridWang, HL=35604926700en_HK
dc.identifier.scopusauthoridShen, JG=7404929947en_HK
dc.identifier.scopusauthoridChen, Y=36463185300en_HK
dc.identifier.scopusauthoridTam, PK=7202539421en_HK

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