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Article: Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells

TitlePolysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells
Authors
Issue Date2010
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/home
Citation
Chinese Medicine, 2010, v. 5 How to Cite?
AbstractBackground: Polysaccharopeptide (PSP) from Coriolus versicolor (Yunzhi) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT).Methods: We used bivariate bromodeoxyuridine/propidium iodide (BrdUrd/PI) flow cytometry analysis to measure the relative movement (RM) of the BrdUrd positively labeled cells and DNA synthesis time (Ts) on the HL-60 cell line. We used annexin V/PI flow cytometry analysis to quantify the viable, necrotic and apoptotic cells. The expression of cyclin E and cyclin B1 was determined with annexin V/PI flow cytometry and western blotting. Human peripheral blood mononuclear cells were used to test the cytotoxicity of PSP and CPT.Results: PSP reduced cellular proliferation; inhibited cells progression through both S and G 2 phase, reduced 3H-thymidine uptake and prolonged DNA synthesis time (Ts) in HL-60 cells. PSP-pretreated cells enhanced the cytotoxicity of CPT. The sensitivity of cells to the cytotoxic effects of CPT was seen to be the highest in the S-phase and to a small extent of the G 2 phase of the cell cycle. On the other hand, no cell death (measured by annexin V/PI) was evident with the normal human peripheral blood mononuclear cells with treatment of either PSP or CPT.Conclusion: The present study shows that PSP increases the sensitization of the HL-60 cells to undergo effective apoptotic cell death induced by CPT. The pattern of sensitivity of cancer cells is similar to that of HL-60 cells. PSP rapidly arrests and/or kills cells in S-phase and did not interfere with the anticancer action of CPT. PSP is a potential adjuvant to treat human leukemia as rapidly proliferating tumors is characterized by a high proportion of S-phase cells. © 2010 Wan et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/127451
ISSN
2015 Impact Factor: 1.58
2015 SCImago Journal Rankings: 0.655
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorWan, JMen_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorYang, Xen_HK
dc.contributor.authorJiang, Pen_HK
dc.contributor.authorWong, LLen_HK
dc.date.accessioned2010-10-31T13:26:19Z-
dc.date.available2010-10-31T13:26:19Z-
dc.date.issued2010en_HK
dc.identifier.citationChinese Medicine, 2010, v. 5en_HK
dc.identifier.issn1749-8546en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127451-
dc.description.abstractBackground: Polysaccharopeptide (PSP) from Coriolus versicolor (Yunzhi) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT).Methods: We used bivariate bromodeoxyuridine/propidium iodide (BrdUrd/PI) flow cytometry analysis to measure the relative movement (RM) of the BrdUrd positively labeled cells and DNA synthesis time (Ts) on the HL-60 cell line. We used annexin V/PI flow cytometry analysis to quantify the viable, necrotic and apoptotic cells. The expression of cyclin E and cyclin B1 was determined with annexin V/PI flow cytometry and western blotting. Human peripheral blood mononuclear cells were used to test the cytotoxicity of PSP and CPT.Results: PSP reduced cellular proliferation; inhibited cells progression through both S and G 2 phase, reduced 3H-thymidine uptake and prolonged DNA synthesis time (Ts) in HL-60 cells. PSP-pretreated cells enhanced the cytotoxicity of CPT. The sensitivity of cells to the cytotoxic effects of CPT was seen to be the highest in the S-phase and to a small extent of the G 2 phase of the cell cycle. On the other hand, no cell death (measured by annexin V/PI) was evident with the normal human peripheral blood mononuclear cells with treatment of either PSP or CPT.Conclusion: The present study shows that PSP increases the sensitization of the HL-60 cells to undergo effective apoptotic cell death induced by CPT. The pattern of sensitivity of cancer cells is similar to that of HL-60 cells. PSP rapidly arrests and/or kills cells in S-phase and did not interfere with the anticancer action of CPT. PSP is a potential adjuvant to treat human leukemia as rapidly proliferating tumors is characterized by a high proportion of S-phase cells. © 2010 Wan et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/homeen_HK
dc.relation.ispartofChinese Medicineen_HK
dc.rightsChinese Medicine. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titlePolysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1749-8546&volume=5&issue=16&spage=&epage=&date=2010&atitle=Polysaccharopeptides+derived+from+Coriolus+versicolor+potentiate+the+S-phase+specific+cytotoxicity+of+Camptothecin+(CPT)+on+human+leukemia+HL-60+cellsen_HK
dc.identifier.emailWan, JM: jmfwan@hku.hken_HK
dc.identifier.authorityWan, JM=rp00798en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1749-8546-5-16en_HK
dc.identifier.pmid20423495-
dc.identifier.pmcidPMC2874562-
dc.identifier.scopuseid_2-s2.0-77951235284en_HK
dc.identifier.hkuros180048en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77951235284&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue16en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWan, JM=8930305000en_HK
dc.identifier.scopusauthoridSit, WH=8528923000en_HK
dc.identifier.scopusauthoridYang, X=14014496500en_HK
dc.identifier.scopusauthoridJiang, P=36147603700en_HK
dc.identifier.scopusauthoridWong, LL=36128985900en_HK

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