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Article: Genomic structure and transcriptional regulation of grass carp calmodulin gene

TitleGenomic structure and transcriptional regulation of grass carp calmodulin gene
Authors
KeywordsCalmodulin
Grass carp
Housekeeping gene
Promoter
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2009, v. 390 n. 3, p. 827-833 How to Cite?
AbstractA fish calmodulin (CaM) gene was characterized for the first time in grass carp. The CaM gene is about 12-Kb in size with identical intron/exon organization as that of mammalian CaM genes. When compared to mammalian counterparts, the 5′-promoter region of grass carp CaM gene contains a TATA box and has a much lower GC content and CpG dinucleotide frequency. Interestingly, the 5′-promoter of carp CaM gene is AT-rich with multiple IRS elements and putative binding sites for Pit-1, Sp1/Sp3 and AP1. Using luciferase reporter assay, a potent silencer region was identified in the distal region of grass carp CaM promoter. Besides, the CaM promoter activity could be upregulated by IGF but suppressed by PACAP, forskolin and over-expression of Sp1 and Sp3. These findings, taken together, indicate that grass carp CaM gene does not exhibit the typical features of housekeeping genes and its expression is under the control of hormone factors, presumably by coupling with the appropriate signaling pathways/transcription factors.
Persistent Identifierhttp://hdl.handle.net/10722/127450
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council (Hong Kong)
University Research Committee (University of Hong Kong)
Funding Information:

The project was supported by Grants to Dr. A.O.L. Wong from Research Grant Council (Hong Kong) and University Research Committee (University of Hong Kong).

References

 

DC FieldValueLanguage
dc.contributor.authorHuo, Len_HK
dc.contributor.authorWong, AOLen_HK
dc.date.accessioned2010-10-31T13:26:16Z-
dc.date.available2010-10-31T13:26:16Z-
dc.date.issued2009en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2009, v. 390 n. 3, p. 827-833en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/127450-
dc.description.abstractA fish calmodulin (CaM) gene was characterized for the first time in grass carp. The CaM gene is about 12-Kb in size with identical intron/exon organization as that of mammalian CaM genes. When compared to mammalian counterparts, the 5′-promoter region of grass carp CaM gene contains a TATA box and has a much lower GC content and CpG dinucleotide frequency. Interestingly, the 5′-promoter of carp CaM gene is AT-rich with multiple IRS elements and putative binding sites for Pit-1, Sp1/Sp3 and AP1. Using luciferase reporter assay, a potent silencer region was identified in the distal region of grass carp CaM promoter. Besides, the CaM promoter activity could be upregulated by IGF but suppressed by PACAP, forskolin and over-expression of Sp1 and Sp3. These findings, taken together, indicate that grass carp CaM gene does not exhibit the typical features of housekeeping genes and its expression is under the control of hormone factors, presumably by coupling with the appropriate signaling pathways/transcription factors.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCalmodulinen_HK
dc.subjectGrass carpen_HK
dc.subjectHousekeeping geneen_HK
dc.subjectPromoteren_HK
dc.subject.mesh5' Flanking Region-
dc.subject.meshCalmodulin - genetics-
dc.subject.meshCarps - genetics-
dc.subject.meshGene Expression Regulation-
dc.subject.meshTranscription, Genetic-
dc.titleGenomic structure and transcriptional regulation of grass carp calmodulin geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=390&issue=3&spage=827&epage=833&date=2009&atitle=Genomic+structure+and+transcriptional+regulation+of+grass+carp+calmodulin+gene-
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.bbrc.2009.10.057en_HK
dc.identifier.pmid19853581-
dc.identifier.scopuseid_2-s2.0-70449705809en_HK
dc.identifier.hkuros173667en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70449705809&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume390en_HK
dc.identifier.issue3en_HK
dc.identifier.spage827en_HK
dc.identifier.epage833en_HK
dc.identifier.isiWOS:000272516700087-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHuo, L=9275343500en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK
dc.identifier.citeulike6004562-

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