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Article: Insufficient activity of MAPK pathway is a key monitor of Kidney-Yang Deficiency Syndrome.

TitleInsufficient activity of MAPK pathway is a key monitor of Kidney-Yang Deficiency Syndrome.
Authors
Issue Date2009
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/acm
Citation
Journal Of Alternative And Complementary Medicine (New York, N.Y.), 2009, v. 15 n. 6, p. 653-660 How to Cite?
AbstractOBJECTIVE: To explore the genetic characteristics and molecular regulator of Kidney-Yang Deficiency Syndrome (KDS). DESIGN: A typical KDS family was collected using a questionnaire of cold feeling and a 40-item scoring table of KDS based on Traditional Chinese Medicine (TCM), by single-blind method repeated annually over three years. Their transcriptomes were assayed by microarray and validated by RT-PCR and ELISA. Simultaneously, 10 healthy volunteers were recruited as controls and the same protocols were performed. RESULTS: This typical KDS family has 35 members, of whom 11 were evaluated as having severe KDS and 6 as having common KDS. Results of the cDNA microarray revealed that there were 420 genes/expressed sequence tags differentially expressed in KDS transcriptomes, indicating a global functional impairment in the mass-energy-information carrying network of KDS patients, involving energy metabolism, signal transduction, development, cell cycle, and immunity. Pathway analysis by gene set enrichment assay (GSEA) and other tools demonstrated that mitogenic activated protein kinase (MAPK) is among the most insufficiently activated pathways, while the oxidative phosphorylation and glycolysis/gluconeogenesis pathways, the two main pathways relevant to ATP synthesis, were among the most excessively activated pathways in KDS patients. Results of RT-PCR and ELISA confirmed the status of insufficient activity of the MAPK pathway. CONCLUSION: KDS patients undergo overall attenuated functions in the mass-energy-information carrying network. The marked low level of energy output in KDS may be primarily attributed to the insufficient activity of the MAPK pathway, which may be a key monitor for the abnormal energy metabolism and other impaired activities in KDS.
Persistent Identifierhttp://hdl.handle.net/10722/127441
ISSN
ISI Accession Number ID
Funding AgencyGrant Number
National Scientific Fund Committee (NSFC) of China30371709
`30582288
Funding Information:

We are indebted to the National Scientific Fund Committee (NSFC) of China for financial support (30371709 and 30582288). The microarray experiment was performed by the CapitalBio Cooperation, Beijing, China.

 

DC FieldValueLanguage
dc.contributor.authorDing, WJen_HK
dc.contributor.authorYan, SLen_HK
dc.contributor.authorZeng, YZen_HK
dc.contributor.authorLi, WHen_HK
dc.contributor.authorDuan, Aen_HK
dc.contributor.authorZheng, TEen_HK
dc.contributor.authorLiu, Men_HK
dc.contributor.authorTan, CEen_HK
dc.contributor.authorTeng, Xen_HK
dc.contributor.authorWan, Jen_HK
dc.date.accessioned2010-10-31T13:25:46Z-
dc.date.available2010-10-31T13:25:46Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Alternative And Complementary Medicine (New York, N.Y.), 2009, v. 15 n. 6, p. 653-660en_HK
dc.identifier.issn1557-7708en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127441-
dc.description.abstractOBJECTIVE: To explore the genetic characteristics and molecular regulator of Kidney-Yang Deficiency Syndrome (KDS). DESIGN: A typical KDS family was collected using a questionnaire of cold feeling and a 40-item scoring table of KDS based on Traditional Chinese Medicine (TCM), by single-blind method repeated annually over three years. Their transcriptomes were assayed by microarray and validated by RT-PCR and ELISA. Simultaneously, 10 healthy volunteers were recruited as controls and the same protocols were performed. RESULTS: This typical KDS family has 35 members, of whom 11 were evaluated as having severe KDS and 6 as having common KDS. Results of the cDNA microarray revealed that there were 420 genes/expressed sequence tags differentially expressed in KDS transcriptomes, indicating a global functional impairment in the mass-energy-information carrying network of KDS patients, involving energy metabolism, signal transduction, development, cell cycle, and immunity. Pathway analysis by gene set enrichment assay (GSEA) and other tools demonstrated that mitogenic activated protein kinase (MAPK) is among the most insufficiently activated pathways, while the oxidative phosphorylation and glycolysis/gluconeogenesis pathways, the two main pathways relevant to ATP synthesis, were among the most excessively activated pathways in KDS patients. Results of RT-PCR and ELISA confirmed the status of insufficient activity of the MAPK pathway. CONCLUSION: KDS patients undergo overall attenuated functions in the mass-energy-information carrying network. The marked low level of energy output in KDS may be primarily attributed to the insufficient activity of the MAPK pathway, which may be a key monitor for the abnormal energy metabolism and other impaired activities in KDS.en_HK
dc.languageengen_HK
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/acm-
dc.relation.ispartofJournal of alternative and complementary medicine (New York, N.Y.)en_HK
dc.rightsThis is a copy of an article published in the Journal of Alternative & Complementary Medicine © 2009 [copyright Mary Ann Liebert, Inc.]; Journal of Alternative & Complementary Medicine is available online at: http://www.liebertonline.com.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBody Temperature - genetics-
dc.subject.meshEnergy Metabolism - genetics-
dc.subject.meshExtracellular Signal-Regulated MAP Kinases - genetics - metabolism-
dc.subject.meshKidney Diseases - metabolism-
dc.subject.meshMAP Kinase Signaling System - genetics - physiology-
dc.titleInsufficient activity of MAPK pathway is a key monitor of Kidney-Yang Deficiency Syndrome.en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1075-5535&volume=15&issue=6&spage=653&epage=660&date=2009&atitle=Insufficient+activity+of+MAPK+pathway+is+a+key+monitor+of+Kidney-Yang+Deficiency+Syndrome-
dc.identifier.emailWan, J: jmfwan@hku.hken_HK
dc.identifier.authorityWan, J=rp00798en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1089/acm.2008.0364-
dc.identifier.pmid19500008-
dc.identifier.scopuseid_2-s2.0-69449084291en_HK
dc.identifier.hkuros180070en_HK
dc.identifier.hkuros155270-
dc.identifier.volume15en_HK
dc.identifier.issue6en_HK
dc.identifier.spage653en_HK
dc.identifier.epage660en_HK
dc.identifier.isiWOS:000267016200010-
dc.identifier.scopusauthoridDing, WJ=26639234000en_HK
dc.identifier.scopusauthoridYan, SL=33167837600en_HK
dc.identifier.scopusauthoridZeng, YZ=35217125600en_HK
dc.identifier.scopusauthoridLi, WH=24348010700en_HK
dc.identifier.scopusauthoridDuan, A=36122748500en_HK
dc.identifier.scopusauthoridZheng, TE=36124454100en_HK
dc.identifier.scopusauthoridLiu, M=55210687200en_HK
dc.identifier.scopusauthoridTan, CE=34067930500en_HK
dc.identifier.scopusauthoridTeng, X=24081680800en_HK
dc.identifier.scopusauthoridWan, J=8930305000en_HK

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