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Article: Regulatory properties of polysaccharopeptide derived from Coriolus versicolor and its combined effect with ciclosporin on the homeostasis of human lymphocytes

TitleRegulatory properties of polysaccharopeptide derived from Coriolus versicolor and its combined effect with ciclosporin on the homeostasis of human lymphocytes
Authors
Keywordsciclosporin
Coriolus versicolor
lymphocyte
polysaccharopeptide
proteoglycan
Issue Date2010
PublisherPharmaceutical Press. The Journal's web site is located at http://www.pharmpress.com/jpp
Citation
Journal Of Pharmacy And Pharmacology, 2010, v. 62 n. 8, p. 1028-1036 How to Cite?
AbstractObjectives Lymphocyte homoeostasis is essential in inflammatory and autoimmune diseases. In search of natural fungal metabolites with effects on lymphocyte homoeostasis, we recently reported that polysaccharopeptide (PSP) from Coriolus versicolor exhibited ciclosporin-like activity in controlling aberrant lymphocyte activation. This object of this study was to investigate its effect on lymphocyte homoeostasis. This was done by investigating the mechanistic actions of PSP in relation to ciclosporin by performing cell cycle and cell death analysis of human lymphocytes in vitro. Methods We investigated the effect of PSP in the presence and absence of ciclosporin on cell proliferation, cell cycle, cell death, immunophenotype and cell cycle regulatory proteins in human lymphocytes. Key findings The data showed that PSP exhibited homoeostatic activity by promoting and inhibiting the proliferation of resting and phytohaemagglutinin (PHA)-stimulated lymphocytes, respectively. PHA-stimulated lymphocytes exhibited G0/G1 cell cycle arrest that was accompanied by a reduction of cyclin E expression with PSP treatment. Both PSP and ciclosporin blocked the reduction of the CD4/CD8 ratio in stimulated lymphocytes. PSP did not induce cell death in human lymphocytes, but the suppression of the Fasreceptor suggested a protective role of PSP against extrinsic cell death signals. These homoeostatic effects were more potent with combined PSP and ciclosporin treatment than with either fungal metabolite alone. Conclusions Collectively, the results reveal certain novel effects of PSP in lymphocyte homoeostasis and suggest potential as a specific immunomodulatory adjuvant for clinical applications in the treatment of autoimmune diseases. © 2010 The Authors. Journal compilation.
Persistent Identifierhttp://hdl.handle.net/10722/127431
ISSN
2015 Impact Factor: 2.363
2015 SCImago Journal Rankings: 0.686
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong, and Hong Kong Health Care Association Ltd, Hong Kong, SAR, P. R. ChinaHKU 7511/03M
Funding Information:

This work was supported in part by RGC Grants No. HKU 7511/03M obtained from The University of Hong Kong, and Hong Kong Health Care Association Ltd, Hong Kong, SAR, P. R. China.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLee, CLen_HK
dc.contributor.authorJiang, Pen_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorYang, Xen_HK
dc.contributor.authorWan, JMFen_HK
dc.date.accessioned2010-10-31T13:25:13Z-
dc.date.available2010-10-31T13:25:13Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Pharmacy And Pharmacology, 2010, v. 62 n. 8, p. 1028-1036en_HK
dc.identifier.issn0022-3573en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127431-
dc.description.abstractObjectives Lymphocyte homoeostasis is essential in inflammatory and autoimmune diseases. In search of natural fungal metabolites with effects on lymphocyte homoeostasis, we recently reported that polysaccharopeptide (PSP) from Coriolus versicolor exhibited ciclosporin-like activity in controlling aberrant lymphocyte activation. This object of this study was to investigate its effect on lymphocyte homoeostasis. This was done by investigating the mechanistic actions of PSP in relation to ciclosporin by performing cell cycle and cell death analysis of human lymphocytes in vitro. Methods We investigated the effect of PSP in the presence and absence of ciclosporin on cell proliferation, cell cycle, cell death, immunophenotype and cell cycle regulatory proteins in human lymphocytes. Key findings The data showed that PSP exhibited homoeostatic activity by promoting and inhibiting the proliferation of resting and phytohaemagglutinin (PHA)-stimulated lymphocytes, respectively. PHA-stimulated lymphocytes exhibited G0/G1 cell cycle arrest that was accompanied by a reduction of cyclin E expression with PSP treatment. Both PSP and ciclosporin blocked the reduction of the CD4/CD8 ratio in stimulated lymphocytes. PSP did not induce cell death in human lymphocytes, but the suppression of the Fasreceptor suggested a protective role of PSP against extrinsic cell death signals. These homoeostatic effects were more potent with combined PSP and ciclosporin treatment than with either fungal metabolite alone. Conclusions Collectively, the results reveal certain novel effects of PSP in lymphocyte homoeostasis and suggest potential as a specific immunomodulatory adjuvant for clinical applications in the treatment of autoimmune diseases. © 2010 The Authors. Journal compilation.en_HK
dc.languageengen_HK
dc.publisherPharmaceutical Press. The Journal's web site is located at http://www.pharmpress.com/jppen_HK
dc.relation.ispartofJournal of Pharmacy and Pharmacologyen_HK
dc.subjectciclosporinen_HK
dc.subjectCoriolus versicoloren_HK
dc.subjectlymphocyteen_HK
dc.subjectpolysaccharopeptideen_HK
dc.subjectproteoglycanen_HK
dc.titleRegulatory properties of polysaccharopeptide derived from Coriolus versicolor and its combined effect with ciclosporin on the homeostasis of human lymphocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3573&volume=62&issue=8&spage=1028&epage=36&date=2010&atitle=Regulatory+properties+of+polysaccharopeptide+derived+from+Coriolus+versicolor+and+its+combined+effect+with+ciclosporin+on+the+homeostasis+of+human+lymphocytes.en_HK
dc.identifier.emailWan, JMF: jmfwan@hku.hken_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1211/jpp.62.08.0009en_HK
dc.identifier.pmid20663037-
dc.identifier.scopuseid_2-s2.0-77954939161en_HK
dc.identifier.hkuros180040en_HK
dc.identifier.hkuros177300-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954939161&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume62en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1028en_HK
dc.identifier.epage1036en_HK
dc.identifier.isiWOS:000280202300009-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectTo determine the mechanisms of apoptosis of polysaccharide peptide (PSP) in human leukemic cell lines and human normal lymphocytes-
dc.identifier.scopusauthoridLee, CL=9277221100en_HK
dc.identifier.scopusauthoridJiang, P=36147603700en_HK
dc.identifier.scopusauthoridSit, WH=8528923000en_HK
dc.identifier.scopusauthoridYang, X=36674059400en_HK
dc.identifier.scopusauthoridWan, JMF=8930305000en_HK

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