File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: 14-3-3 Protein regulates cell adhesion in the seminiferous epithelium of rat testes

Title14-3-3 Protein regulates cell adhesion in the seminiferous epithelium of rat testes
Authors
Issue Date2009
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2009, v. 150 n. 10, p. 4713-4723 How to Cite?
AbstractPolarity proteins have been implicated in regulating and maintaining tight junction (TJ) and cell polarity in epithelia. Here we report 14-3-3θ, the homolog of Caenorhabditis elegans Par5 in mammalian cells, which is known to confer cell polarity at TJ, is found at the apical ectoplasmic specialization (ES), a testis-specific adherens junction type restricted to the Sertoli cell-elongating spermatid interface, in which TJ is absent. 14-3-3θ was shown to play a critical role in conferring cell adhesion at the apical ES. A loss of 14-3-3θ expression at the apical ES was detected in the seminiferous epithelium before spermiation. Involvement of 14-3-3θ in Sertoli cell adhesion was confirmed by its knockdown by RNA interference in Sertoli cells cultured in vitro with established TJ permeability barrier that mimicked the blood-testis barrier (BTB) in vivo. Mislocalization of N-cadherin and zonula occludens-1, but not α- and β-catenins, was observed after 14-3-3θ knockdown in Sertoli cells, moving from the cell-cell interface to cytosol, indicating a disruption of cell adhesion. Studies by endocytosis assay illustrated that this loss of cell adhesion was mediated by an increase in the kinetics of endocytosis of N-cadherin and junctional adhesion molecule-A at the BTB, which may represent a general mechanism by which polarity proteins regulate cell adhesion. In summary, the testis is using 14-3-3θ to regulate cell adhesion at the apical ES to facilitate spermiation and at the BTB to facilitate the transit of preleptotene spermatocytes at stages VIII-IX of the epithelial cycle. 14-3-3θ may act as a molecular switch that coordinates these two cellular events in the seminiferous epithelium during spermatogenesis. Copyright © 2009 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/127426
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of Health, National, Institute of Child Health and Human DevelopmentR01HD056034
R03HD051512
Hong Kong Research Grants CouncilHKU 7693/07M
Funding Information:

Disclosure Summary: The authors have nothing to disclose.

References

 

DC FieldValueLanguage
dc.contributor.authorWong, EWPen_HK
dc.contributor.authorSun, Sen_HK
dc.contributor.authorLi, MWMen_HK
dc.contributor.authorLee, WMen_HK
dc.contributor.authorCheng, CYen_HK
dc.date.accessioned2010-10-31T13:24:56Z-
dc.date.available2010-10-31T13:24:56Z-
dc.date.issued2009en_HK
dc.identifier.citationEndocrinology, 2009, v. 150 n. 10, p. 4713-4723en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127426-
dc.description.abstractPolarity proteins have been implicated in regulating and maintaining tight junction (TJ) and cell polarity in epithelia. Here we report 14-3-3θ, the homolog of Caenorhabditis elegans Par5 in mammalian cells, which is known to confer cell polarity at TJ, is found at the apical ectoplasmic specialization (ES), a testis-specific adherens junction type restricted to the Sertoli cell-elongating spermatid interface, in which TJ is absent. 14-3-3θ was shown to play a critical role in conferring cell adhesion at the apical ES. A loss of 14-3-3θ expression at the apical ES was detected in the seminiferous epithelium before spermiation. Involvement of 14-3-3θ in Sertoli cell adhesion was confirmed by its knockdown by RNA interference in Sertoli cells cultured in vitro with established TJ permeability barrier that mimicked the blood-testis barrier (BTB) in vivo. Mislocalization of N-cadherin and zonula occludens-1, but not α- and β-catenins, was observed after 14-3-3θ knockdown in Sertoli cells, moving from the cell-cell interface to cytosol, indicating a disruption of cell adhesion. Studies by endocytosis assay illustrated that this loss of cell adhesion was mediated by an increase in the kinetics of endocytosis of N-cadherin and junctional adhesion molecule-A at the BTB, which may represent a general mechanism by which polarity proteins regulate cell adhesion. In summary, the testis is using 14-3-3θ to regulate cell adhesion at the apical ES to facilitate spermiation and at the BTB to facilitate the transit of preleptotene spermatocytes at stages VIII-IX of the epithelial cycle. 14-3-3θ may act as a molecular switch that coordinates these two cellular events in the seminiferous epithelium during spermatogenesis. Copyright © 2009 by The Endocrine Society.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.subject.mesh14-3-3 Proteins - metabolism-
dc.subject.meshBlood-Testis Barrier - physiology-
dc.subject.meshCell Adhesion-
dc.subject.meshEndocytosis-
dc.subject.meshSeminiferous Epithelium - physiology-
dc.title14-3-3 Protein regulates cell adhesion in the seminiferous epithelium of rat testesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=150&issue=10&spage=4713&epage=4723&date=2009&atitle=14-3-3+regulates+cell+adhesion+in+the+seminiferous+epitheliumen_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1210/en.2009-0427en_HK
dc.identifier.pmid19608648-
dc.identifier.pmcidPMC2754685-
dc.identifier.scopuseid_2-s2.0-70349331263en_HK
dc.identifier.hkuros179021en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349331263&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume150en_HK
dc.identifier.issue10en_HK
dc.identifier.spage4713en_HK
dc.identifier.epage4723en_HK
dc.identifier.isiWOS:000270021700026-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, EWP=23029194700en_HK
dc.identifier.scopusauthoridSun, S=34977782600en_HK
dc.identifier.scopusauthoridLi, MWM=27168276300en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK
dc.identifier.scopusauthoridCheng, CY=7404797787en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats