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Article: Rapid detection of reassortment of pandemic H1N1/2009 influenza virus

TitleRapid detection of reassortment of pandemic H1N1/2009 influenza virus
Authors
Issue Date2010
PublisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.org
Citation
Clinical Chemistry, 2010, v. 56 n. 8, p. 1340-1344 How to Cite?
AbstractBACKGROUND: Influenza viruses can generate novel reassortants in coinfected cells. The global circulation and occasional introductions of pandemic H1N1/2009 virus in humans and in pigs, respectively, may allow this virus to reassort with other influenza viruses. These possible reassortment events might alter virulence and/or transmissibility of the new reassortants. Investigations to detect such possible reassortants should be included as a part of pandemic influenza surveillance plans. METHODS: We established a real-time reverse-transcription (RT)-PCR-based strategy for the detection of reassortment of pandemic H1N1/2009 virus. Singleplex SYBR green-based RT-PCR assays specific for each gene segment of pandemic H1N1/2009 were developed. These assays were evaluated with influenza viruses of various genetic backgrounds. RESULTS: All human pandemic H1N1 (n = 27) and all seasonal human (n = 58) isolates were positive and negative, respectively, for all 8 segments. Of 48 swine influenza viruses isolated from our ongoing surveillance program of influenza viruses in swine, 10 were positive in all reactions. All 8 viral segments of these 10 samples were confirmed to be of pandemic H1N1 origin, indicating that these were caused by zoonotic transmissions from human to pigs. The 38 swine viruses that were nonpandemic H1N1/2009 had 1-6 gene segments positive in the tests. Further characterization of these nonpandemic H1N1/2009 swine viruses indicated that these PCR-positive genes were the precursor genes of the pandemic H1N1/2009 virus. CONCLUSIONS: Our results demonstrated that these assays can detect reintroductions of pandemic H1N1/ 2009 virus in pigs. These assays might be useful screening tools for identifying viral reassortants derived from pandemic H1N1/2009 or its precursors.
Persistent Identifierhttp://hdl.handle.net/10722/127309
ISSN
2015 Impact Factor: 7.457
2015 SCImago Journal Rankings: 2.472
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council of Hong KongHKU 773408M
University Grants Committee Hong KongAoE/M-12/06
Food and Health Bureau
NIH (NIAID)HHSN266200700005C
Funding Information:

Research Funding: L. L. M. Poon, Research Grant Council of Hong Kong (HKU 773408M). This work was supported by the Area of Excellence Scheme of the University Grants Committee Hong Kong (grant AoE/M-12/06), the Research Fund for the Control of Infections Disease Commissioned Project Food and Health Bureau, and the NIH (NIAID contract HHSN266200700005C).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorMak, PWYen_HK
dc.contributor.authorLi, OTWen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorCheung, CLen_HK
dc.contributor.authorMa, Een_HK
dc.contributor.authorYen, HLen_HK
dc.contributor.authorVijaykrishna, Den_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2010-10-31T13:18:00Z-
dc.date.available2010-10-31T13:18:00Z-
dc.date.issued2010en_HK
dc.identifier.citationClinical Chemistry, 2010, v. 56 n. 8, p. 1340-1344en_HK
dc.identifier.issn0009-9147en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127309-
dc.description.abstractBACKGROUND: Influenza viruses can generate novel reassortants in coinfected cells. The global circulation and occasional introductions of pandemic H1N1/2009 virus in humans and in pigs, respectively, may allow this virus to reassort with other influenza viruses. These possible reassortment events might alter virulence and/or transmissibility of the new reassortants. Investigations to detect such possible reassortants should be included as a part of pandemic influenza surveillance plans. METHODS: We established a real-time reverse-transcription (RT)-PCR-based strategy for the detection of reassortment of pandemic H1N1/2009 virus. Singleplex SYBR green-based RT-PCR assays specific for each gene segment of pandemic H1N1/2009 were developed. These assays were evaluated with influenza viruses of various genetic backgrounds. RESULTS: All human pandemic H1N1 (n = 27) and all seasonal human (n = 58) isolates were positive and negative, respectively, for all 8 segments. Of 48 swine influenza viruses isolated from our ongoing surveillance program of influenza viruses in swine, 10 were positive in all reactions. All 8 viral segments of these 10 samples were confirmed to be of pandemic H1N1 origin, indicating that these were caused by zoonotic transmissions from human to pigs. The 38 swine viruses that were nonpandemic H1N1/2009 had 1-6 gene segments positive in the tests. Further characterization of these nonpandemic H1N1/2009 swine viruses indicated that these PCR-positive genes were the precursor genes of the pandemic H1N1/2009 virus. CONCLUSIONS: Our results demonstrated that these assays can detect reintroductions of pandemic H1N1/ 2009 virus in pigs. These assays might be useful screening tools for identifying viral reassortants derived from pandemic H1N1/2009 or its precursors.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.orgen_HK
dc.relation.ispartofClinical Chemistryen_HK
dc.titleRapid detection of reassortment of pandemic H1N1/2009 influenza virusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0009-9147&volume=56&spage=&epage=&date=2010&atitle=Rapid+Detection+of+Reassortment+of+Pandemic+H1N1/2009+Influenza+Virusen_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.emailYen, HL: hyen@hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.identifier.authorityYen, HL=rp00304en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1373/clinchem.2010.149179en_HK
dc.identifier.pmid20567024-
dc.identifier.scopuseid_2-s2.0-77955262410en_HK
dc.identifier.hkuros173143en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955262410&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume56en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1340en_HK
dc.identifier.epage1344en_HK
dc.identifier.isiWOS:000280501400023-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectCodon biases of avian and human influenza A viruses-
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridMak, PWY=36022676500en_HK
dc.identifier.scopusauthoridLi, OTW=16230718400en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridCheung, CL=34975244700en_HK
dc.identifier.scopusauthoridMa, E=36572109200en_HK
dc.identifier.scopusauthoridYen, HL=7102476668en_HK
dc.identifier.scopusauthoridVijaykrishna, D=12752817700en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK

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