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Conference Paper: Real-time quantifications of dominant anaerobes in an upflow reactor by polymerase chain reaction using a TaqMan gene probe

TitleReal-time quantifications of dominant anaerobes in an upflow reactor by polymerase chain reaction using a TaqMan gene probe
Authors
Issue Date2008
PublisherIWA Publishing. The Journal's web site is located at http://www.iwaponline.com/wst/default.htm
Citation
The 11th World Congress on Anaerobic Digestion (AD11), Brisbane, Australia, 23-27 September 2007. In Water Science and Technology, 2008, v. 57 n. 11, p. 1851-1855 How to Cite?
AbstractThis study was conducted to demonstrate the application of quantitative real-time polymerase chain reaction (qRT-PCR) for the quantification of dominant bacteria in an anaerobic reactor using a designed TaqMan probe. A novel group of uncultured thermophilic bacteria affiliated with Thermotogales was first found in a phenol-degrading sludge from a 55 degrees C upflow anaerobic sludge blanket (UASB) reactor, which effectively removed 99% of phenol at loading of 0.51 g-phenol l(-1) d(-1) h of hydraulic retention. A TaqMan probe was then designed targeting this group of Thermotogales affiliated bacteria (TAB), and used to monitor its concentration in the reactors. Results showed that the TAB population in the 55 degrees C reactor increased proportional to the phenol degrading rate. Results also showed that the TAB population ranged 3.5-9.9% in the 55 degrees C phenol-degrading sludge, but only 0.0044% in the 37 degrees C sludge and 0.000086% in the 26 degrees C sludge.
DescriptionSession PP6C - Microbial Diversity 2
Persistent Identifierhttp://hdl.handle.net/10722/127244
ISSN
2015 Impact Factor: 1.064
2015 SCImago Journal Rankings: 0.469
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLang, DWen_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-10-31T13:14:23Z-
dc.date.available2010-10-31T13:14:23Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 11th World Congress on Anaerobic Digestion (AD11), Brisbane, Australia, 23-27 September 2007. In Water Science and Technology, 2008, v. 57 n. 11, p. 1851-1855en_HK
dc.identifier.issn0273-1223-
dc.identifier.urihttp://hdl.handle.net/10722/127244-
dc.descriptionSession PP6C - Microbial Diversity 2-
dc.description.abstractThis study was conducted to demonstrate the application of quantitative real-time polymerase chain reaction (qRT-PCR) for the quantification of dominant bacteria in an anaerobic reactor using a designed TaqMan probe. A novel group of uncultured thermophilic bacteria affiliated with Thermotogales was first found in a phenol-degrading sludge from a 55 degrees C upflow anaerobic sludge blanket (UASB) reactor, which effectively removed 99% of phenol at loading of 0.51 g-phenol l(-1) d(-1) h of hydraulic retention. A TaqMan probe was then designed targeting this group of Thermotogales affiliated bacteria (TAB), and used to monitor its concentration in the reactors. Results showed that the TAB population in the 55 degrees C reactor increased proportional to the phenol degrading rate. Results also showed that the TAB population ranged 3.5-9.9% in the 55 degrees C phenol-degrading sludge, but only 0.0044% in the 37 degrees C sludge and 0.000086% in the 26 degrees C sludge.-
dc.languageengen_HK
dc.publisherIWA Publishing. The Journal's web site is located at http://www.iwaponline.com/wst/default.htm-
dc.relation.ispartofWater Science and Technology-
dc.rightsWater Science and Technology. Copyright © IWA Publishing.-
dc.subject.meshBacteria, Anaerobic - genetics - isolation and purification-
dc.subject.meshBioreactors - microbiology-
dc.subject.meshPolymerase Chain Reaction - methods-
dc.subject.meshSewage - chemistry - microbiology-
dc.subject.meshWater Microbiology-
dc.titleReal-time quantifications of dominant anaerobes in an upflow reactor by polymerase chain reaction using a TaqMan gene probeen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0273-1223&volume=57&spage=1851&epage=1855&date=2008&atitle=Real-time+quantifications+of+dominant+anaerobes+in+an+upflow+reactor+by+polymerase+chain+reaction+using+a+TaqMan+probe-
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hken_HK
dc.identifier.emailFang, HHP: hrechef@hkucc.hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2166/wst.2008.042-
dc.identifier.pmid18547940-
dc.identifier.scopuseid_2-s2.0-47349102249-
dc.identifier.hkuros155504en_HK
dc.identifier.hkuros182674-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-47349102249&selection=ref&src=s&origin=recordpage-
dc.identifier.volume57-
dc.identifier.issue11-
dc.identifier.spage1851-
dc.identifier.epage1855-
dc.identifier.isiWOS:000256799000024-
dc.publisher.placeUnited Kingdom-
dc.description.otherThe 11th World Congress on Anaerobic Digestion (AD11), Brisbane, Australia, 23-27 September 2007. In Water Science and Technology, 2008, v. 57 n. 11, p. 1851-1855-
dc.identifier.scopusauthoridLiang, DW=15835235400-
dc.identifier.scopusauthoridZhang, T=24470677400-
dc.identifier.scopusauthoridFang, HHP=7402542625-

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