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Conference Paper: Plasma microRNA as a potential marker for breast cancer detection

TitlePlasma microRNA as a potential marker for breast cancer detection
Authors
Issue Date2010
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://www.aacrmeetingabstracts.org/
Citation
The 101st Annual Meeting of the American Association for Cancer Research (AACR 2010), Washington, DC., 17-21 April 2010. In AACR Meeting Abstracts, 2010 How to Cite?
AbstractBACKGROUND: Recently, the emergence of small non-protein-coding RNAs, so-called microRNAs (miRNAs), playing important roles in oncogenesis has opened new opportunities for early cancer diagnosis. We have previously shown that microRNAs (miRNAs) in plasma are promising biomarkers for colorectal cancer detection. Here, we investigated whether plasma miRNAs could discriminate patients with and without breast cancer (BC). METHODS: Using TaqMan-based low density miRNA array, miRNAs were profiled from cancerous and adjacent non-cancerous breast tissues, corresponding plasma of 5 BC patients, along with plasma from 5 healthy controls. Marker selection and validation were performed by real-time quantitative RT-PCR on a small set of plasma. Independent set of plasma from 80 BC patients, 20 gastric cancer, 20 lung cancer, 20 colorectal cancer, 20 hepatocellular carcinoma and 50 healthy controls were further validated. RESULTS: Of the panel of 377 miRNAs analyzed, 8 miRNAs (miR-16, miR-21, miR-27a, miR-141, miR-191, miR-200c, miR-210, miR-451) were up-regulated both in plasma and tissue samples of 5 BC patients. All 8 putative miRNA markers were validated on the plasma of 15 BC patients and 15 healthy controls. Only three miRNAs were significantly elevated in this cohort of BC patients (p<0.0005). The plasma levels of the three markers in those 15 BC patients were significantly reduced after surgery (p<0.05). Further validation with an independent set of plasma samples (n=210) indicated that two markers differentiate BC from normal subjects, colorectal cancer, gastric cancer, hepatocellular carcinoma and lung cancer. These two markers yielded a combined receiver operating characteristic curve area of 88.5%, the sensitivity was 92% and the specificity was 72% in discriminating BC from control subjects. CONCLUSIONS: Plasma miRNAs significantly elevated in BC patients are identified. This can be a novel noninvasive molecular marker for BC screening.
DescriptionPoster Session 4 - MicroRNA Profiling in Cancer 2: abstract no. 3027
Persistent Identifierhttp://hdl.handle.net/10722/126952
ISSN

 

DC FieldValueLanguage
dc.contributor.authorNg, EKOen_HK
dc.contributor.authorLeung, Cen_HK
dc.contributor.authorAu, Sen_HK
dc.contributor.authorChan, Aen_HK
dc.contributor.authorWong, Cen_HK
dc.contributor.authorMa, Een_HK
dc.contributor.authorPang, RWCen_HK
dc.contributor.authorChua, Den_HK
dc.contributor.authorChu, KMen_HK
dc.contributor.authorLaw, WLen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorKwong, Aen_HK
dc.date.accessioned2010-10-31T12:57:58Z-
dc.date.available2010-10-31T12:57:58Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 101st Annual Meeting of the American Association for Cancer Research (AACR 2010), Washington, DC., 17-21 April 2010. In AACR Meeting Abstracts, 2010en_HK
dc.identifier.issn1948-3279-
dc.identifier.urihttp://hdl.handle.net/10722/126952-
dc.descriptionPoster Session 4 - MicroRNA Profiling in Cancer 2: abstract no. 3027-
dc.description.abstractBACKGROUND: Recently, the emergence of small non-protein-coding RNAs, so-called microRNAs (miRNAs), playing important roles in oncogenesis has opened new opportunities for early cancer diagnosis. We have previously shown that microRNAs (miRNAs) in plasma are promising biomarkers for colorectal cancer detection. Here, we investigated whether plasma miRNAs could discriminate patients with and without breast cancer (BC). METHODS: Using TaqMan-based low density miRNA array, miRNAs were profiled from cancerous and adjacent non-cancerous breast tissues, corresponding plasma of 5 BC patients, along with plasma from 5 healthy controls. Marker selection and validation were performed by real-time quantitative RT-PCR on a small set of plasma. Independent set of plasma from 80 BC patients, 20 gastric cancer, 20 lung cancer, 20 colorectal cancer, 20 hepatocellular carcinoma and 50 healthy controls were further validated. RESULTS: Of the panel of 377 miRNAs analyzed, 8 miRNAs (miR-16, miR-21, miR-27a, miR-141, miR-191, miR-200c, miR-210, miR-451) were up-regulated both in plasma and tissue samples of 5 BC patients. All 8 putative miRNA markers were validated on the plasma of 15 BC patients and 15 healthy controls. Only three miRNAs were significantly elevated in this cohort of BC patients (p<0.0005). The plasma levels of the three markers in those 15 BC patients were significantly reduced after surgery (p<0.05). Further validation with an independent set of plasma samples (n=210) indicated that two markers differentiate BC from normal subjects, colorectal cancer, gastric cancer, hepatocellular carcinoma and lung cancer. These two markers yielded a combined receiver operating characteristic curve area of 88.5%, the sensitivity was 92% and the specificity was 72% in discriminating BC from control subjects. CONCLUSIONS: Plasma miRNAs significantly elevated in BC patients are identified. This can be a novel noninvasive molecular marker for BC screening.-
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://www.aacrmeetingabstracts.org/-
dc.relation.ispartofAACR Meeting Abstracts-
dc.titlePlasma microRNA as a potential marker for breast cancer detectionen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailNg, EKO: enders.ng@gmail.comen_HK
dc.identifier.emailLeung, C: geli_candy@hotmail.comen_HK
dc.identifier.emailMa, E: eskma@hksh.comen_HK
dc.identifier.emailPang, RWC: robertap@hku.hken_HK
dc.identifier.emailChua, D: danielchua@hksh.comen_HK
dc.identifier.emailChu, KM: chukm@hkucc.hku.hken_HK
dc.identifier.emailLaw, WL: lawwl@hkucc.hku.hken_HK
dc.identifier.emailPoon, RTP: poontp@hkucc.hku.hken_HK
dc.identifier.emailKwong, A: avakwong@HKUCC.hku.hken_HK
dc.identifier.hkuros172818en_HK
dc.description.otherThe 101st Annual Meeting of the American Association for Cancer Research (AACR), Washington, DC., 17-21 April 2010. In AACR Meeting Abstracts, 2010-

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