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Conference Paper: Differential expression of microRNAs in plasma of colorectal cancer patients: a potential marker for colorectal cancer screening

TitleDifferential expression of microRNAs in plasma of colorectal cancer patients: a potential marker for colorectal cancer screening
Authors
Issue Date2009
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
The 2009 American Gastroenterlogical Association (AGA) Institute and Digestive Disease Week (DDW), Chicago, IL., 29 May-4 Jun 2009. In Gastroenterology, 2009, v. 136 n. 5 suppl.1, p. A165, abstract no. 1070 How to Cite?
AbstractOBJECTIVE: MicroRNAs (miRNA), a class of small non-coding RNAs, play important roles in cancers and have been shown to have great cancer diagnostic potential. Since cell-free miRNAs are recently detected in the plasma and serum, we investigated whether plasma miRNAs enable to discriminate patients with and without colorectal cancer (CRC). METHODS: This study was divided into three phases: (i) Marker discovery using real-time PCR-based miRNA profiling on plasma, corresponding cancerous and adjacent non-cancerous colonic tissues of 5 CRC patients, along with plasma from 5 healthy controls. (ii) miRNA marker selection and validation by real-time quantitative RT-PCR on plasma from 25 CRC and 20 healthy controls. (iii) Validation on an independent set of plasma from 90 CRC patients, 20 gastric cancers, 20 inflammatory bowel disease and 50 healthy controls. RESULTS: Of the panel of 95 miRNAs analyzed, 5 miRNAs were up-regulated both in plasma and tissue samples. All 5 miRNAs were validated on the plasma of 25 CRC patients and 20 healthy controls. Both miR-17-3p and miR-92 were significantly elevated in CRC patients (p<0.0005). The plasma levels of these markers were significantly reduced after surgery in 10 CRC patients (p<0.05). Further validation with an independent set of plasma samples (n=180) indicated that miR-92 differentiates CRC from gastric cancer, IBD and normal subjects. This marker yielded a receiver operating characteristic curve area of 88.5%. At a cutoff of 240, the sensitivity was 89% and the specificity was 70% in discriminating CRC from control subjects. Conclusions: Our findings demonstrated that plasma miRNAs have great potential for CRC screening. This opens up a new class of molecular markers for CRC diagnosis.
DescriptionTopic Forum: Oral Sessions - Scientific Sessions: Microrna and digestive cancers, Oral Presentation no. 1070
Persistent Identifierhttp://hdl.handle.net/10722/126948
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170

 

DC FieldValueLanguage
dc.contributor.authorSung, JJen_HK
dc.contributor.authorChong, WSen_HK
dc.contributor.authorJin, HCen_HK
dc.contributor.authorLam, EKYen_HK
dc.contributor.authorShin, VYen_HK
dc.contributor.authorYu, Jen_HK
dc.contributor.authorPoon, TCWen_HK
dc.contributor.authorNg, SSen_HK
dc.contributor.authorNg, EKOen_HK
dc.date.accessioned2010-10-31T12:57:45Z-
dc.date.available2010-10-31T12:57:45Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 2009 American Gastroenterlogical Association (AGA) Institute and Digestive Disease Week (DDW), Chicago, IL., 29 May-4 Jun 2009. In Gastroenterology, 2009, v. 136 n. 5 suppl.1, p. A165, abstract no. 1070en_HK
dc.identifier.issn0016-5085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/126948-
dc.descriptionTopic Forum: Oral Sessions - Scientific Sessions: Microrna and digestive cancers, Oral Presentation no. 1070-
dc.description.abstractOBJECTIVE: MicroRNAs (miRNA), a class of small non-coding RNAs, play important roles in cancers and have been shown to have great cancer diagnostic potential. Since cell-free miRNAs are recently detected in the plasma and serum, we investigated whether plasma miRNAs enable to discriminate patients with and without colorectal cancer (CRC). METHODS: This study was divided into three phases: (i) Marker discovery using real-time PCR-based miRNA profiling on plasma, corresponding cancerous and adjacent non-cancerous colonic tissues of 5 CRC patients, along with plasma from 5 healthy controls. (ii) miRNA marker selection and validation by real-time quantitative RT-PCR on plasma from 25 CRC and 20 healthy controls. (iii) Validation on an independent set of plasma from 90 CRC patients, 20 gastric cancers, 20 inflammatory bowel disease and 50 healthy controls. RESULTS: Of the panel of 95 miRNAs analyzed, 5 miRNAs were up-regulated both in plasma and tissue samples. All 5 miRNAs were validated on the plasma of 25 CRC patients and 20 healthy controls. Both miR-17-3p and miR-92 were significantly elevated in CRC patients (p<0.0005). The plasma levels of these markers were significantly reduced after surgery in 10 CRC patients (p<0.05). Further validation with an independent set of plasma samples (n=180) indicated that miR-92 differentiates CRC from gastric cancer, IBD and normal subjects. This marker yielded a receiver operating characteristic curve area of 88.5%. At a cutoff of 240, the sensitivity was 89% and the specificity was 70% in discriminating CRC from control subjects. Conclusions: Our findings demonstrated that plasma miRNAs have great potential for CRC screening. This opens up a new class of molecular markers for CRC diagnosis.-
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro-
dc.relation.ispartofGastroenterologyen_HK
dc.titleDifferential expression of microRNAs in plasma of colorectal cancer patients: a potential marker for colorectal cancer screeningen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-5085&volume=136&issue=5, suppl.1&spage=A165, abstract no. 1070&epage=&date=2009&atitle=Differential+expression+of+microRNAs+in+plasma+of+colorectal+cancer+patients:+a+potential+marker+for+colorectal+cancer+screeningen_HK
dc.identifier.emailShin, VY: vyshin@hku.hken_HK
dc.identifier.emailNg, EKO: enders.ng@gmail.comen_HK
dc.identifier.authorityNg, EKO=rp01364en_HK
dc.identifier.doi10.1016/S0016-5085(09)60743-5-
dc.identifier.hkuros181438en_HK
dc.identifier.volume136en_HK
dc.identifier.issue5 suppl.1en_HK
dc.identifier.spageA165, abstract no. 1070en_HK
dc.identifier.epageA165, abstract no. 1070en_HK
dc.publisher.placeUnited States-

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