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Conference Paper: Effects of survival and proliferation of smooth muscle cells (SMC) by supercooling and re-warming

TitleEffects of survival and proliferation of smooth muscle cells (SMC) by supercooling and re-warming
Authors
Issue Date2007
PublisherSouthern Association for Vascular Surgery.
Citation
The 31st Annual Meeting of the Southern Association for Vascular Surgery, Rio Grande, Puerto Rico, USA., 17-20 January 2007. How to Cite?
AbstractBackground: SMC plays a critical role in neointimal hyperplasia after angioplasty. Studies showed that cryoplasty produces less restenosis clinically, however, its underlying mechanisms are still not well understood. In this study, we investigated the effects of SMC survival and proliferation after supercooling and re-warming in an in-vitro model simulating cryoplasty. Methods: Bovine aortic smooth cells were cultured in 6 well plates with medium supplemented with 10% fetal bovine serum. The samples were supercooled to around -10oC for 0 and 60 seconds using a conduction cooling stage and then re-warmed in an incubator at 37oC for 0,6, 12 or 24 hours. TUNEL assay and BrdU were used to measure the degree of apoptosis and proliferation respectively. Western blot measured the activation of p70S6 kinase and p44/42MAP kinase and were quantified using densitometry. Results are given as mean + standard error of mean and analysed by ANOVA. Results: Significant increased SMC apoptosis and decreased proliferation were observed with increasing supercooling and re-warming time. Both p70S6 kinase and p44/42 MAP kinase showed no changes with supercooling and re-warming. Conclusions: The decreased proliferation and increased apoptosis of SMC after supercooling and re-warming may explain why cryoplasty can produce less restenosis. No significant changes in p70S6 kinase and p44/42 MAP kinase level may indicate that other pathways may be involved in both transcription and translation control of SMC after supercooling and re-warming.
DescriptionBasic Science Session: B4
Persistent Identifierhttp://hdl.handle.net/10722/126934

 

DC FieldValueLanguage
dc.contributor.authorYiu, WKen_HK
dc.contributor.authorCheng, SWKen_HK
dc.contributor.authorSumpio, BEen_HK
dc.date.accessioned2010-10-31T12:57:00Z-
dc.date.available2010-10-31T12:57:00Z-
dc.date.issued2007en_HK
dc.identifier.citationThe 31st Annual Meeting of the Southern Association for Vascular Surgery, Rio Grande, Puerto Rico, USA., 17-20 January 2007.en_HK
dc.identifier.urihttp://hdl.handle.net/10722/126934-
dc.descriptionBasic Science Session: B4-
dc.description.abstractBackground: SMC plays a critical role in neointimal hyperplasia after angioplasty. Studies showed that cryoplasty produces less restenosis clinically, however, its underlying mechanisms are still not well understood. In this study, we investigated the effects of SMC survival and proliferation after supercooling and re-warming in an in-vitro model simulating cryoplasty. Methods: Bovine aortic smooth cells were cultured in 6 well plates with medium supplemented with 10% fetal bovine serum. The samples were supercooled to around -10oC for 0 and 60 seconds using a conduction cooling stage and then re-warmed in an incubator at 37oC for 0,6, 12 or 24 hours. TUNEL assay and BrdU were used to measure the degree of apoptosis and proliferation respectively. Western blot measured the activation of p70S6 kinase and p44/42MAP kinase and were quantified using densitometry. Results are given as mean + standard error of mean and analysed by ANOVA. Results: Significant increased SMC apoptosis and decreased proliferation were observed with increasing supercooling and re-warming time. Both p70S6 kinase and p44/42 MAP kinase showed no changes with supercooling and re-warming. Conclusions: The decreased proliferation and increased apoptosis of SMC after supercooling and re-warming may explain why cryoplasty can produce less restenosis. No significant changes in p70S6 kinase and p44/42 MAP kinase level may indicate that other pathways may be involved in both transcription and translation control of SMC after supercooling and re-warming.-
dc.languageengen_HK
dc.publisherSouthern Association for Vascular Surgery.-
dc.relation.ispartofAnnual Meeting of the Southern Association for Vascular Surgery-
dc.titleEffects of survival and proliferation of smooth muscle cells (SMC) by supercooling and re-warmingen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYiu, WK: yiuwaiki@hotmail.comen_HK
dc.identifier.emailCheng, SWK: wkcheng@hkucc.hku.hken_HK
dc.identifier.hkuros176084en_HK
dc.description.otherThe 31st Annual Meeting of the Southern Association for Vascular Surgery, Rio Grande, Puerto Rico, USA., 17-20 January 2007.-

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