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Conference Paper: Differential survival responses of vascular smooth muscle cells (SMC) and endothelial cells (EC) towards different cycles of supercooling and re-warming

TitleDifferential survival responses of vascular smooth muscle cells (SMC) and endothelial cells (EC) towards different cycles of supercooling and re-warming
Authors
KeywordsMedical sciences
Surgery
Issue Date2007
PublisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/ASH
Citation
Conjoint Scientific Congress of the Royal College of Surgeons of England and the College of Surgeons of Hong Kong, Hong Kong, 6–7 May 2007. In Surgical Practice, 2007, v. 11 n. 4, p. A5, abstract no. 2 How to Cite?
AbstractBACKGROUND: Cryoplasty combines mechanical dilation with supercooling of the vessel used in the treatment of peripheral arterial disease. The mechanism of action is not well defined. This study investigates the optimal supercooling and re-warming cycles by studying the survival responses of SMC and EC. ATERIAL AND METHODS: Bovine aortic SMC and EC were cultured separately in 6 well plates with medium supplemented with 10% fetal bovine serum. In the 1 cycle treatment group, the cells were supercooled for 60 s to -10°C and then re-warmed rapidly in a water bath at 37°C for another 60 s. The samples were then put into an incubator at 37°C for 0, 6, 12 and 24 h. Two cycles treatment was done by supercooling and re-warming the cells twice. TUNEL assay and immunohistochemistry (IHC) and western blot (WB) were used to measure apoptosis and Akt activation. Results are given as mean +/- standard error of mean and analysed by ANOVA. RESULTS: Both EC and SMC showed increasing apoptosis with re-warming time and number of cycle treatment with significantly higher rate in SMC (P < 0.05). Akt activation decreased in SMC (P < 0.05) but significantly peaked at 6 h by threefold in EC (P < 0.05). CONCLUSION: The higher apoptotic rate with longer re-warming times and repeated treatment cycle in SMC, together with lower Akt activation may explain why cryoplasty can produce lower restenosis rate. This information will be useful in determining optimal treatment regimen for cryoplasty.
Persistent Identifierhttp://hdl.handle.net/10722/126925
ISSN
2013 Impact Factor: 0.172
2015 SCImago Journal Rankings: 0.121
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYiu, WKen_HK
dc.contributor.authorCheng, SWKen_HK
dc.contributor.authorSumpio, BEen_HK
dc.date.accessioned2010-10-31T12:56:30Z-
dc.date.available2010-10-31T12:56:30Z-
dc.date.issued2007en_HK
dc.identifier.citationConjoint Scientific Congress of the Royal College of Surgeons of England and the College of Surgeons of Hong Kong, Hong Kong, 6–7 May 2007. In Surgical Practice, 2007, v. 11 n. 4, p. A5, abstract no. 2en_HK
dc.identifier.issn1744-1625-
dc.identifier.urihttp://hdl.handle.net/10722/126925-
dc.description.abstractBACKGROUND: Cryoplasty combines mechanical dilation with supercooling of the vessel used in the treatment of peripheral arterial disease. The mechanism of action is not well defined. This study investigates the optimal supercooling and re-warming cycles by studying the survival responses of SMC and EC. ATERIAL AND METHODS: Bovine aortic SMC and EC were cultured separately in 6 well plates with medium supplemented with 10% fetal bovine serum. In the 1 cycle treatment group, the cells were supercooled for 60 s to -10°C and then re-warmed rapidly in a water bath at 37°C for another 60 s. The samples were then put into an incubator at 37°C for 0, 6, 12 and 24 h. Two cycles treatment was done by supercooling and re-warming the cells twice. TUNEL assay and immunohistochemistry (IHC) and western blot (WB) were used to measure apoptosis and Akt activation. Results are given as mean +/- standard error of mean and analysed by ANOVA. RESULTS: Both EC and SMC showed increasing apoptosis with re-warming time and number of cycle treatment with significantly higher rate in SMC (P < 0.05). Akt activation decreased in SMC (P < 0.05) but significantly peaked at 6 h by threefold in EC (P < 0.05). CONCLUSION: The higher apoptotic rate with longer re-warming times and repeated treatment cycle in SMC, together with lower Akt activation may explain why cryoplasty can produce lower restenosis rate. This information will be useful in determining optimal treatment regimen for cryoplasty.-
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/ASH-
dc.relation.ispartofSurgical Practice-
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.subjectMedical sciences-
dc.subjectSurgery-
dc.titleDifferential survival responses of vascular smooth muscle cells (SMC) and endothelial cells (EC) towards different cycles of supercooling and re-warmingen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1744-1625&volume=11&issue=4&spage=A5&epage=A11&date=2007&atitle=Differential+survival+responses+of+vascular+smooth+muscle+cells+(SMC)+and+endothelial+cells+(EC)+towards+different+cycles+of+supercooling+and+re-warming-
dc.identifier.emailYiu, WK: yiuwaiki@hotmail.comen_HK
dc.identifier.emailCheng, SWK: wkcheng@hkucc.hku.hken_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1744-1633.2007.00378.x-
dc.identifier.hkuros176087en_HK
dc.identifier.volume11-
dc.identifier.issue4-
dc.identifier.spageA5-
dc.identifier.epageA5-
dc.identifier.isiWOS:000254624200011-
dc.publisher.placeAustralia-
dc.description.otherConjoint Scientific Congress of the Royal College of Surgeons of England and the College of Surgeons of Hong Kong, Hong Kong, 6–7 May 2007. In Surgical Practice, 2007, v. 11 n. 4, p. A5, abstract no. 2-

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