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Article: Role of miR-143 regulating DNA methyltransferases 3A in breast cancer

TitleRole of miR-143 regulating DNA methyltransferases 3A in breast cancer
Authors
KeywordsMedical sciences
Oncology
Issue Date2010
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 2010, v. 69 n. 24, suppl.: abstract no. 3148 How to Cite?
AbstractBackground and Aims: MicroRNAs (miRNAs) are 19-25-nucleotides regulatory non-protein-coding RNA molecules that regulate the expressions of a wide variety of genes including some involved in cancer development. In particular, decreased expression of miR-143 has been reported in various human cancers including colorectal cancer and B-cell lymphomas. The aim of this study was to elucidate the role of miR-143 dysregulation in breast cancer.Methods: Expression levels of human mature microRNAs (miRNAs) were compared with paired breast carcinomas and adjacent normal tissues by TaqMan real-time PCR based expression arrays. Decreased expression of miR-143 was further confirmed in breast cancer cell lines and paired breast tumors and normal adjacent tissues by qRT-PCR. Potential targets of miR-143 were defined. The functional effect of miR-143 and its targets was performed in human breast cancer cell lines to confirm target association.Results: Down-regulation of miR-143 was verified in both human breast cancer cell lines and 80% (12/15) of breast tumors (P < 0.001). DNA methyltranferase 3A (DNMT3A), one of a key enzyme involved in DNA methylation, was defined as a potential target of miR-143 by in-silico analysis. Overexpression of miR-143 in breast cancer cell lines down-regulated expression of DNMT3A, decreased tumor cell growth by MTT assay and soft agar colony formation assay. DNMT3A was demonstrated to be a direct target of miR-143 by luciferase reporter assay. Inverse correlation between DNMT3A protein and miR-143 was found in tumor and normal breast tissues.Conclusions: In this study, we show for the first time in breast cancer that miR-143 specifically targeted DNMT3A and the expression of miR-143 was inversely correlated with DNMT3A expression. Our findings demonstrated that down-regulation of miR-143 and up-regulation of DNMT3A are significant changes in breast tumors. These findings indicate a tumor suppressive role of miR-143 in epigenetic aberration of breast cancer.
Persistent Identifierhttp://hdl.handle.net/10722/126911
ISSN
2014 Impact Factor: 9.329
2013 SCImago Journal Rankings: 5.627

 

DC FieldValueLanguage
dc.contributor.authorNg, Een_HK
dc.contributor.authorKwong, Aen_HK
dc.contributor.authorTsang, Wen_HK
dc.contributor.authorLeung, Cen_HK
dc.contributor.authorWong, Cen_HK
dc.contributor.authorKwok, Ten_HK
dc.contributor.authorMa, Een_HK
dc.date.accessioned2010-10-31T12:55:44Z-
dc.date.available2010-10-31T12:55:44Z-
dc.date.issued2010en_HK
dc.identifier.citationCancer Research, 2010, v. 69 n. 24, suppl.: abstract no. 3148en_HK
dc.identifier.issn0008-5472en_HK
dc.identifier.urihttp://hdl.handle.net/10722/126911-
dc.description.abstractBackground and Aims: MicroRNAs (miRNAs) are 19-25-nucleotides regulatory non-protein-coding RNA molecules that regulate the expressions of a wide variety of genes including some involved in cancer development. In particular, decreased expression of miR-143 has been reported in various human cancers including colorectal cancer and B-cell lymphomas. The aim of this study was to elucidate the role of miR-143 dysregulation in breast cancer.Methods: Expression levels of human mature microRNAs (miRNAs) were compared with paired breast carcinomas and adjacent normal tissues by TaqMan real-time PCR based expression arrays. Decreased expression of miR-143 was further confirmed in breast cancer cell lines and paired breast tumors and normal adjacent tissues by qRT-PCR. Potential targets of miR-143 were defined. The functional effect of miR-143 and its targets was performed in human breast cancer cell lines to confirm target association.Results: Down-regulation of miR-143 was verified in both human breast cancer cell lines and 80% (12/15) of breast tumors (P < 0.001). DNA methyltranferase 3A (DNMT3A), one of a key enzyme involved in DNA methylation, was defined as a potential target of miR-143 by in-silico analysis. Overexpression of miR-143 in breast cancer cell lines down-regulated expression of DNMT3A, decreased tumor cell growth by MTT assay and soft agar colony formation assay. DNMT3A was demonstrated to be a direct target of miR-143 by luciferase reporter assay. Inverse correlation between DNMT3A protein and miR-143 was found in tumor and normal breast tissues.Conclusions: In this study, we show for the first time in breast cancer that miR-143 specifically targeted DNMT3A and the expression of miR-143 was inversely correlated with DNMT3A expression. Our findings demonstrated that down-regulation of miR-143 and up-regulation of DNMT3A are significant changes in breast tumors. These findings indicate a tumor suppressive role of miR-143 in epigenetic aberration of breast cancer.-
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/-
dc.relation.ispartofCancer Researchen_HK
dc.subjectMedical sciences-
dc.subjectOncology-
dc.titleRole of miR-143 regulating DNA methyltransferases 3A in breast canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-5472&volume=69&issue=24 suppl: abstract no. 3148&spage=&epage=&date=2010&atitle=Role+of+miR-143+regulating+DNA+methyltransferases+3A+in+breast+canceren_HK
dc.identifier.emailNg, E: ngko@hku.hk, enders.ng@gmail.comen_HK
dc.identifier.emailKwong, A: avakwong@HKUCC.hku.hken_HK
dc.identifier.emailLeung, C: geli_candy@hotmail.comen_HK
dc.identifier.emailMa, E: eskma@HKUCC.hku.hken_HK
dc.identifier.authorityNg, E=rp01364en_HK
dc.identifier.doi10.1158/0008-5472.SABCS-09-3148-
dc.identifier.hkuros181442en_HK
dc.identifier.volume69en_HK
dc.identifier.issue24 suppl: abstract no. 3148en_HK

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