Conference Paper: Nitroglycerin reduces TNF-alpha toxicity to endothelial cells but compromises the protective effects of propofol

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TitleNitroglycerin reduces TNF-alpha toxicity to endothelial cells but compromises the protective effects of propofol
AuthorsLiu, HM
Lei, S
Luo, T
Xia, ZY
Liu, Y
Leung, GPH
Vanhoutte, PM
Irwin, MG
Xia, Z
KeywordsBiology
Issue Date2010
PublisherFederation of American Societies for Experimental Biology.
CitationExperiment Biology Meeting (EB 2010), Anaheim, CA., 24-28 April 2010. In The FASEB Journal, 2010, v. 24 Meeting abstract suppl., abstract no. 959 [How to Cite?]
AbstractSupplementation of nitroglycerin (NTG) may increase cardiomyocyte death during ischemia (Histol Histopathol 2009;24(12):1487–98) and the underlying mechanism is unknown. Vascular endothelial cell apoptosis precedes cardiomyocyte apoptosis during myocardial ischemia-reperfusion, a situation that is associated with increased tumor necrosis factor-α (TNF) production. We postulated that NTG may exacerbate TNF induced endothelial cell apoptosis by enhancing nitrative stress. Cultured human umbilical vein endothelial cells were either not treated (control), treated with TNF (40 ng/ml) alone or TNF in the presence of NTG, propofol (an anesthetic that scavenges peroxynitrite), or NTG plus propofol, respectively, for 16 hr. TNF increased endothelial cell apoptosis, accompanied by increased protein expression of nitrotyrosine, a footprint of peroxynitrite and an index of nitrative stress. Both NTG and propofol attenuated TNF-induced cell apoptosis, however this protective effect diminished when NTG and propofol were used in combination and this was accompanied by increased nitrotyrosine expression and protein kinase C-β2 phosphorylation at site ser660. NTG may have compromised propofol cellular protection by counteracting its peroxynitrite scavenging capacity.
ISSN0892-6638
2011 Impact Factor: 5.712
2011 SCImago Journal Rankings: 0.680
DC Field
Value
dc.contributor.authorLiu, HM
dc.contributor.authorLei, S
dc.contributor.authorLuo, T
dc.contributor.authorXia, ZY
dc.contributor.authorLiu, Y
dc.contributor.authorLeung, GPH
dc.contributor.authorVanhoutte, PM
dc.contributor.authorIrwin, MG
dc.contributor.authorXia, Z
dc.date.accessioned2010-10-31T12:54:57Z
dc.date.available2010-10-31T12:54:57Z
dc.date.issued2010
dc.description.abstractSupplementation of nitroglycerin (NTG) may increase cardiomyocyte death during ischemia (Histol Histopathol 2009;24(12):1487–98) and the underlying mechanism is unknown. Vascular endothelial cell apoptosis precedes cardiomyocyte apoptosis during myocardial ischemia-reperfusion, a situation that is associated with increased tumor necrosis factor-α (TNF) production. We postulated that NTG may exacerbate TNF induced endothelial cell apoptosis by enhancing nitrative stress. Cultured human umbilical vein endothelial cells were either not treated (control), treated with TNF (40 ng/ml) alone or TNF in the presence of NTG, propofol (an anesthetic that scavenges peroxynitrite), or NTG plus propofol, respectively, for 16 hr. TNF increased endothelial cell apoptosis, accompanied by increased protein expression of nitrotyrosine, a footprint of peroxynitrite and an index of nitrative stress. Both NTG and propofol attenuated TNF-induced cell apoptosis, however this protective effect diminished when NTG and propofol were used in combination and this was accompanied by increased nitrotyrosine expression and protein kinase C-β2 phosphorylation at site ser660. NTG may have compromised propofol cellular protection by counteracting its peroxynitrite scavenging capacity.
dc.description.naturelink_to_OA_fulltext
dc.description.otherExperiment Biology Meeting (EB 2010), Anaheim, CA., 24-28 April 2010. In The FASEB Journal, 2010, v. 24 Meeting abstract suppl., abstract no. 959
dc.description.otherOpen Access Journal
dc.identifier.citationExperiment Biology Meeting (EB 2010), Anaheim, CA., 24-28 April 2010. In The FASEB Journal, 2010, v. 24 Meeting abstract suppl., abstract no. 959 [How to Cite?]
dc.identifier.hkuros171206
dc.identifier.issn0892-6638
2011 Impact Factor: 5.712
2011 SCImago Journal Rankings: 0.680
dc.identifier.issueMeeting abstract suppl.
dc.identifier.urihttp://hdl.handle.net/10722/126898
dc.identifier.volume24
dc.languageeng
dc.publisherFederation of American Societies for Experimental Biology.
dc.relation.ispartofThe FASEB Journal
dc.subjectBiology
dc.titleNitroglycerin reduces TNF-alpha toxicity to endothelial cells but compromises the protective effects of propofol
dc.typeConference_Paper