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Conference Paper: Genomic changes caused by chronic exposure to high cholesterol or fish oil in native and regenerated porcine coronary endothelial cells
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TitleGenomic changes caused by chronic exposure to high cholesterol or fish oil in native and regenerated porcine coronary endothelial cells
 
AuthorsVanhoutte, PM
Lee, MYK
Wang, Y
Tse, HF
 
KeywordsPharmacy and pharmacology environmental studies
Toxicology and environmental safety
 
Issue Date2010
 
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PTO
 
CitationThe 16th World Congress on Basic and Clinical Pharmacology (WorldPharma2010), Copenhagen, Denmark, 17-23 July 2010. In Basic & Clinical Pharmacology & Toxicology, 2010, v. 107, suppl. 1, p. 633-634 [How to Cite?]
 
AbstractThe genomic changes associated with endothelial lesions caused by balloon angioplasty and hypercholesterolemia or those affected by dietary fish oil in porcine coronary arteries were analyzed using large scale microarray gene expression profiling. Pigs were fed with high cholesterol (CHL) diet or fish oil diet rich in omega-3 unsaturated fatty acids. Arterial endothelial denudation was performed by percutaneous transluminal coronary angioplasty of the left anterior descending artery (LAD). Cells from left circumflex (native cells) and LAD (regenerated cells) were harvested for primary culture and subsequent genomic microarray experiments four weeks after the surgery. The plasma levels of LDL-C, triglycerides and arachidonic acid were elevated while those of HDL-C were reduced in the CHL group. Fish oil treatment reduced the plasma levels of arachidonic acid, LDL-C, HDL-C, and the LDL-C/HDL-C ratio. Only 27 genes showed differential regulations, whereas 385 genes were altered similarly by hypercholesterolemia and regeneration. In addition, hypercholesterolemia induced 396 gene changes that were independent of endothelial regeneration. Fish oil significantly reduced the number of gene changes associated with regeneration (176 genes). Regeneration-induced changes of 72 genes were either completely or partially blocked by fish oil. Thus, the present study identifies endothelial genomic changes associated with both hypercholesterolemia-induced prelesional responses and postlesional damage caused by regeneration. The genes responsible for the potential protective effects of fish oil were determined.
 
DescriptionFocused Conference Group: P15 – Endotheliumin Health and Disease. Paper No. 2268
 
ISSN1742-7835
2012 Impact Factor: 2.124
2012 SCImago Journal Rankings: 0.588
 
DC FieldValue
dc.contributor.authorVanhoutte, PM
 
dc.contributor.authorLee, MYK
 
dc.contributor.authorWang, Y
 
dc.contributor.authorTse, HF
 
dc.date.accessioned2010-10-31T12:53:41Z
 
dc.date.available2010-10-31T12:53:41Z
 
dc.date.issued2010
 
dc.description.abstractThe genomic changes associated with endothelial lesions caused by balloon angioplasty and hypercholesterolemia or those affected by dietary fish oil in porcine coronary arteries were analyzed using large scale microarray gene expression profiling. Pigs were fed with high cholesterol (CHL) diet or fish oil diet rich in omega-3 unsaturated fatty acids. Arterial endothelial denudation was performed by percutaneous transluminal coronary angioplasty of the left anterior descending artery (LAD). Cells from left circumflex (native cells) and LAD (regenerated cells) were harvested for primary culture and subsequent genomic microarray experiments four weeks after the surgery. The plasma levels of LDL-C, triglycerides and arachidonic acid were elevated while those of HDL-C were reduced in the CHL group. Fish oil treatment reduced the plasma levels of arachidonic acid, LDL-C, HDL-C, and the LDL-C/HDL-C ratio. Only 27 genes showed differential regulations, whereas 385 genes were altered similarly by hypercholesterolemia and regeneration. In addition, hypercholesterolemia induced 396 gene changes that were independent of endothelial regeneration. Fish oil significantly reduced the number of gene changes associated with regeneration (176 genes). Regeneration-induced changes of 72 genes were either completely or partially blocked by fish oil. Thus, the present study identifies endothelial genomic changes associated with both hypercholesterolemia-induced prelesional responses and postlesional damage caused by regeneration. The genes responsible for the potential protective effects of fish oil were determined.
 
dc.descriptionFocused Conference Group: P15 – Endotheliumin Health and Disease. Paper No. 2268
 
dc.description.otherThe 16th World Congress on Basic and Clinical Pharmacology (WorldPharma2010), Copenhagen, Denmark, 17-23 July 2010. In Basic & Clinical Pharmacology & Toxicology, 2010, v. 107, suppl. 1, p. 633-634
 
dc.identifier.citationThe 16th World Congress on Basic and Clinical Pharmacology (WorldPharma2010), Copenhagen, Denmark, 17-23 July 2010. In Basic & Clinical Pharmacology & Toxicology, 2010, v. 107, suppl. 1, p. 633-634 [How to Cite?]
 
dc.identifier.epage634
 
dc.identifier.hkuros175356
 
dc.identifier.issn1742-7835
2012 Impact Factor: 2.124
2012 SCImago Journal Rankings: 0.588
 
dc.identifier.openurl
 
dc.identifier.spage633
 
dc.identifier.urihttp://hdl.handle.net/10722/126875
 
dc.identifier.volume107, suppl. 1
 
dc.languageeng
 
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PTO
 
dc.relation.ispartofBasic & Clinical Pharmacology & Toxicology
 
dc.rightsThe definitive version is available at www.blackwell-synergy.com
 
dc.subjectPharmacy and pharmacology environmental studies
 
dc.subjectToxicology and environmental safety
 
dc.titleGenomic changes caused by chronic exposure to high cholesterol or fish oil in native and regenerated porcine coronary endothelial cells
 
dc.typeConference_Paper
 
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