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Conference Paper: Genomic changes caused by chronic exposure to high cholesterol or fish oil in native and regenerated porcine coronary endothelial cells

TitleGenomic changes caused by chronic exposure to high cholesterol or fish oil in native and regenerated porcine coronary endothelial cells
Authors
KeywordsPharmacy and pharmacology environmental studies
Toxicology and environmental safety
Issue Date2010
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PTO
Citation
The 16th World Congress on Basic and Clinical Pharmacology (WorldPharma2010), Copenhagen, Denmark, 17-23 July 2010. In Basic & Clinical Pharmacology & Toxicology, 2010, v. 107, suppl. 1, p. 633-634 How to Cite?
AbstractThe genomic changes associated with endothelial lesions caused by balloon angioplasty and hypercholesterolemia or those affected by dietary fish oil in porcine coronary arteries were analyzed using large scale microarray gene expression profiling. Pigs were fed with high cholesterol (CHL) diet or fish oil diet rich in omega-3 unsaturated fatty acids. Arterial endothelial denudation was performed by percutaneous transluminal coronary angioplasty of the left anterior descending artery (LAD). Cells from left circumflex (native cells) and LAD (regenerated cells) were harvested for primary culture and subsequent genomic microarray experiments four weeks after the surgery. The plasma levels of LDL-C, triglycerides and arachidonic acid were elevated while those of HDL-C were reduced in the CHL group. Fish oil treatment reduced the plasma levels of arachidonic acid, LDL-C, HDL-C, and the LDL-C/HDL-C ratio. Only 27 genes showed differential regulations, whereas 385 genes were altered similarly by hypercholesterolemia and regeneration. In addition, hypercholesterolemia induced 396 gene changes that were independent of endothelial regeneration. Fish oil significantly reduced the number of gene changes associated with regeneration (176 genes). Regeneration-induced changes of 72 genes were either completely or partially blocked by fish oil. Thus, the present study identifies endothelial genomic changes associated with both hypercholesterolemia-induced prelesional responses and postlesional damage caused by regeneration. The genes responsible for the potential protective effects of fish oil were determined.
DescriptionFocused Conference Group: P15 – Endotheliumin Health and Disease. Paper No. 2268
Persistent Identifierhttp://hdl.handle.net/10722/126875
ISSN
2014 Impact Factor: 2.377
2014 SCImago Journal Rankings: 0.634

 

DC FieldValueLanguage
dc.contributor.authorVanhoutte, PMen_HK
dc.contributor.authorLee, MYKen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorTse, HFen_HK
dc.date.accessioned2010-10-31T12:53:41Z-
dc.date.available2010-10-31T12:53:41Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 16th World Congress on Basic and Clinical Pharmacology (WorldPharma2010), Copenhagen, Denmark, 17-23 July 2010. In Basic & Clinical Pharmacology & Toxicology, 2010, v. 107, suppl. 1, p. 633-634en_HK
dc.identifier.issn1742-7835-
dc.identifier.urihttp://hdl.handle.net/10722/126875-
dc.descriptionFocused Conference Group: P15 – Endotheliumin Health and Disease. Paper No. 2268-
dc.description.abstractThe genomic changes associated with endothelial lesions caused by balloon angioplasty and hypercholesterolemia or those affected by dietary fish oil in porcine coronary arteries were analyzed using large scale microarray gene expression profiling. Pigs were fed with high cholesterol (CHL) diet or fish oil diet rich in omega-3 unsaturated fatty acids. Arterial endothelial denudation was performed by percutaneous transluminal coronary angioplasty of the left anterior descending artery (LAD). Cells from left circumflex (native cells) and LAD (regenerated cells) were harvested for primary culture and subsequent genomic microarray experiments four weeks after the surgery. The plasma levels of LDL-C, triglycerides and arachidonic acid were elevated while those of HDL-C were reduced in the CHL group. Fish oil treatment reduced the plasma levels of arachidonic acid, LDL-C, HDL-C, and the LDL-C/HDL-C ratio. Only 27 genes showed differential regulations, whereas 385 genes were altered similarly by hypercholesterolemia and regeneration. In addition, hypercholesterolemia induced 396 gene changes that were independent of endothelial regeneration. Fish oil significantly reduced the number of gene changes associated with regeneration (176 genes). Regeneration-induced changes of 72 genes were either completely or partially blocked by fish oil. Thus, the present study identifies endothelial genomic changes associated with both hypercholesterolemia-induced prelesional responses and postlesional damage caused by regeneration. The genes responsible for the potential protective effects of fish oil were determined.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PTO-
dc.relation.ispartofBasic & Clinical Pharmacology & Toxicologyen_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subjectPharmacy and pharmacology environmental studies-
dc.subjectToxicology and environmental safety-
dc.titleGenomic changes caused by chronic exposure to high cholesterol or fish oil in native and regenerated porcine coronary endothelial cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1742-7835&volume=107, suppl. 1&spage=633&epage=634&date=2010&atitle=Genomic+changes+caused+by+chronic+exposure+to+high+cholesterol+or+fish+oil+in+native+and+regenerated+porcine+coronary+endothelial+cells-
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.emailLee, MYK: leemary@hkucc.hku.hken_HK
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailTse, HF: hftse@hkucc.hku.hk-
dc.identifier.hkuros175356en_HK
dc.identifier.volume107, suppl. 1en_HK
dc.identifier.spage633en_HK
dc.identifier.epage634en_HK
dc.description.otherThe 16th World Congress on Basic and Clinical Pharmacology (WorldPharma2010), Copenhagen, Denmark, 17-23 July 2010. In Basic & Clinical Pharmacology & Toxicology, 2010, v. 107, suppl. 1, p. 633-634-

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