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Conference Paper: Large-scale induction and expansion of a novel human alloantigen-specific CD8 regulatory T cells
Title | Large-scale induction and expansion of a novel human alloantigen-specific CD8 regulatory T cells |
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Authors | |
Keywords | Medical sciences Allergology and immunology |
Issue Date | 2009 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yclim |
Citation | The 9th Annual Meeting of the Federation of Clinical Immunology Societies (FOCIS 2009), San Fransisco, CA., 11-15 June 2009. In Clinical Immunology, 2009, v. 131 suppl. 1, p. S163, abstract no. S.114 How to Cite? |
Abstract | Although recent studies have been focused on CD4+ regulatory T cells (Treg), CD8+Treg have also been reported to play important roles in the induction and maintenance of immune tolerance. Adoptive transfer of CD8+Treg in rodents or induction of CD8+Treg in human can prevent or treat allograft rejection and autoimmune diseases. However, no approaches have been reported for the generation of human antigen-specific CD8+Treg at a practical scale for clinical use. Here, we found that two novel CD8+T-cell subsets with different levels of CD8 surface expression: CD8high and CD8low, could be induced from naïve CD8+precursors in vitro by allogeneic CD40-activated B cells, whereas only CD8high T cells were alloantigen-specific Treg with relatively poor alloantigen-specific cytotoxicity. Importantly, alloantigenspecific CD8high Treg could be induced and expanded from naïve CD8+CD25-T cells at a large scale after 3 weeks of culture without exogenous cytokines. These induced alloantigen-specific Treg were CD45RO+and CCR7− memory cells, and expressed Foxp3, CD25, CD27, CD28, and CD62L. The induction and expansion of CD8high Treg by CD40-activated B cells were dependent on endogenously expressed IFN-γ, IL-2, IL-4 and CTLA-4. This approach may facilitate the clinical application of CD8+Treg-based immunotherapy in transplantation and autoimmune diseases. |
Description | This journal supplement is abstracts of FOCIS 2009 |
Persistent Identifier | http://hdl.handle.net/10722/126832 |
ISSN | 2023 Impact Factor: 4.5 2023 SCImago Journal Rankings: 1.359 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zheng, J | en_HK |
dc.contributor.author | Liu, Y | en_HK |
dc.contributor.author | Qin, G | en_HK |
dc.contributor.author | Chan, PL | en_HK |
dc.contributor.author | Mao, H | en_HK |
dc.contributor.author | Lewis, DB | en_HK |
dc.contributor.author | Lau, YL | en_HK |
dc.contributor.author | Tu, W | en_HK |
dc.date.accessioned | 2010-10-31T12:51:14Z | - |
dc.date.available | 2010-10-31T12:51:14Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | The 9th Annual Meeting of the Federation of Clinical Immunology Societies (FOCIS 2009), San Fransisco, CA., 11-15 June 2009. In Clinical Immunology, 2009, v. 131 suppl. 1, p. S163, abstract no. S.114 | en_HK |
dc.identifier.issn | 1521-6616 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/126832 | - |
dc.description | This journal supplement is abstracts of FOCIS 2009 | - |
dc.description.abstract | Although recent studies have been focused on CD4+ regulatory T cells (Treg), CD8+Treg have also been reported to play important roles in the induction and maintenance of immune tolerance. Adoptive transfer of CD8+Treg in rodents or induction of CD8+Treg in human can prevent or treat allograft rejection and autoimmune diseases. However, no approaches have been reported for the generation of human antigen-specific CD8+Treg at a practical scale for clinical use. Here, we found that two novel CD8+T-cell subsets with different levels of CD8 surface expression: CD8high and CD8low, could be induced from naïve CD8+precursors in vitro by allogeneic CD40-activated B cells, whereas only CD8high T cells were alloantigen-specific Treg with relatively poor alloantigen-specific cytotoxicity. Importantly, alloantigenspecific CD8high Treg could be induced and expanded from naïve CD8+CD25-T cells at a large scale after 3 weeks of culture without exogenous cytokines. These induced alloantigen-specific Treg were CD45RO+and CCR7− memory cells, and expressed Foxp3, CD25, CD27, CD28, and CD62L. The induction and expansion of CD8high Treg by CD40-activated B cells were dependent on endogenously expressed IFN-γ, IL-2, IL-4 and CTLA-4. This approach may facilitate the clinical application of CD8+Treg-based immunotherapy in transplantation and autoimmune diseases. | - |
dc.language | eng | en_HK |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yclim | - |
dc.relation.ispartof | Clinical Immunology | en_HK |
dc.subject | Medical sciences | - |
dc.subject | Allergology and immunology | - |
dc.title | Large-scale induction and expansion of a novel human alloantigen-specific CD8 regulatory T cells | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1521-6616&volume=131&issue=suppl. 1 article no. S.114&spage=S163&epage=&date=2009&atitle=Large-scale+Induction+and+Expansion+of+a+Novel+Human+Alloantigen-specific+CD8+Regulatory+T+Cells | en_HK |
dc.identifier.email | Liu, Y: yinpingl@hku.hk | en_HK |
dc.identifier.email | Mao, H: maohw@hkusua.hku.hk | en_HK |
dc.identifier.email | Lau, YL: lauylung@hkucc.hku.hk | en_HK |
dc.identifier.email | Tu, W: wwtu@hkucc.hku.hk | - |
dc.identifier.doi | 10.1016/j.clim.2009.03.483 | - |
dc.identifier.hkuros | 179393 | en_HK |
dc.identifier.hkuros | 163415 | - |
dc.identifier.volume | 131 | en_HK |
dc.identifier.issue | suppl. 1 | - |
dc.identifier.spage | S163, abstract no. S.114 | en_HK |
dc.identifier.epage | S163, abstract no. S.114 | - |
dc.identifier.isi | WOS:000266342300475 | - |
dc.publisher.place | United States | - |
dc.description.other | The 9th Annual Meeting, Federation of Clinical Immunology Societies (FOCIS 2009), San Fransisco, California, USA, 11-15 June 2009. In Clinical Immunology, 2009, v. 131 suppl. 1, p. S163, abstract no. S.114 | - |
dc.identifier.issnl | 1521-6616 | - |