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Conference Paper: Large-scale induction and expansion of a novel human alloantigen-specific CD8 regulatory T cells

TitleLarge-scale induction and expansion of a novel human alloantigen-specific CD8 regulatory T cells
Authors
KeywordsMedical sciences
Allergology and immunology
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yclim
Citation
The 9th Annual Meeting of the Federation of Clinical Immunology Societies (FOCIS 2009), San Fransisco, CA., 11-15 June 2009. In Clinical Immunology, 2009, v. 131 suppl. 1, p. S163, abstract no. S.114 How to Cite?
AbstractAlthough recent studies have been focused on CD4+ regulatory T cells (Treg), CD8+Treg have also been reported to play important roles in the induction and maintenance of immune tolerance. Adoptive transfer of CD8+Treg in rodents or induction of CD8+Treg in human can prevent or treat allograft rejection and autoimmune diseases. However, no approaches have been reported for the generation of human antigen-specific CD8+Treg at a practical scale for clinical use. Here, we found that two novel CD8+T-cell subsets with different levels of CD8 surface expression: CD8high and CD8low, could be induced from naïve CD8+precursors in vitro by allogeneic CD40-activated B cells, whereas only CD8high T cells were alloantigen-specific Treg with relatively poor alloantigen-specific cytotoxicity. Importantly, alloantigenspecific CD8high Treg could be induced and expanded from naïve CD8+CD25-T cells at a large scale after 3 weeks of culture without exogenous cytokines. These induced alloantigen-specific Treg were CD45RO+and CCR7− memory cells, and expressed Foxp3, CD25, CD27, CD28, and CD62L. The induction and expansion of CD8high Treg by CD40-activated B cells were dependent on endogenously expressed IFN-γ, IL-2, IL-4 and CTLA-4. This approach may facilitate the clinical application of CD8+Treg-based immunotherapy in transplantation and autoimmune diseases.
DescriptionThis journal supplement is abstracts of FOCIS 2009
Persistent Identifierhttp://hdl.handle.net/10722/126832
ISSN
2015 Impact Factor: 4.034
2015 SCImago Journal Rankings: 1.848
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZheng, Jen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorQin, Gen_HK
dc.contributor.authorChan, PLen_HK
dc.contributor.authorMao, Hen_HK
dc.contributor.authorLewis, DBen_HK
dc.contributor.authorLau, YLen_HK
dc.contributor.authorTu, Wen_HK
dc.date.accessioned2010-10-31T12:51:14Z-
dc.date.available2010-10-31T12:51:14Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 9th Annual Meeting of the Federation of Clinical Immunology Societies (FOCIS 2009), San Fransisco, CA., 11-15 June 2009. In Clinical Immunology, 2009, v. 131 suppl. 1, p. S163, abstract no. S.114en_HK
dc.identifier.issn1521-6616en_HK
dc.identifier.urihttp://hdl.handle.net/10722/126832-
dc.descriptionThis journal supplement is abstracts of FOCIS 2009-
dc.description.abstractAlthough recent studies have been focused on CD4+ regulatory T cells (Treg), CD8+Treg have also been reported to play important roles in the induction and maintenance of immune tolerance. Adoptive transfer of CD8+Treg in rodents or induction of CD8+Treg in human can prevent or treat allograft rejection and autoimmune diseases. However, no approaches have been reported for the generation of human antigen-specific CD8+Treg at a practical scale for clinical use. Here, we found that two novel CD8+T-cell subsets with different levels of CD8 surface expression: CD8high and CD8low, could be induced from naïve CD8+precursors in vitro by allogeneic CD40-activated B cells, whereas only CD8high T cells were alloantigen-specific Treg with relatively poor alloantigen-specific cytotoxicity. Importantly, alloantigenspecific CD8high Treg could be induced and expanded from naïve CD8+CD25-T cells at a large scale after 3 weeks of culture without exogenous cytokines. These induced alloantigen-specific Treg were CD45RO+and CCR7− memory cells, and expressed Foxp3, CD25, CD27, CD28, and CD62L. The induction and expansion of CD8high Treg by CD40-activated B cells were dependent on endogenously expressed IFN-γ, IL-2, IL-4 and CTLA-4. This approach may facilitate the clinical application of CD8+Treg-based immunotherapy in transplantation and autoimmune diseases.-
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yclim-
dc.relation.ispartofClinical Immunologyen_HK
dc.subjectMedical sciences-
dc.subjectAllergology and immunology-
dc.titleLarge-scale induction and expansion of a novel human alloantigen-specific CD8 regulatory T cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1521-6616&volume=131&issue=suppl. 1 article no. S.114&spage=S163&epage=&date=2009&atitle=Large-scale+Induction+and+Expansion+of+a+Novel+Human+Alloantigen-specific+CD8+Regulatory+T+Cellsen_HK
dc.identifier.emailLiu, Y: yinpingl@hku.hken_HK
dc.identifier.emailMao, H: maohw@hkusua.hku.hken_HK
dc.identifier.emailLau, YL: lauylung@hkucc.hku.hken_HK
dc.identifier.emailTu, W: wwtu@hkucc.hku.hk-
dc.identifier.doi10.1016/j.clim.2009.03.483-
dc.identifier.hkuros179393en_HK
dc.identifier.hkuros163415-
dc.identifier.volume131en_HK
dc.identifier.issuesuppl. 1-
dc.identifier.spageS163, abstract no. S.114en_HK
dc.identifier.epageS163, abstract no. S.114-
dc.identifier.isiWOS:000266342300475-
dc.publisher.placeUnited States-
dc.description.otherThe 9th Annual Meeting, Federation of Clinical Immunology Societies (FOCIS 2009), San Fransisco, California, USA, 11-15 June 2009. In Clinical Immunology, 2009, v. 131 suppl. 1, p. S163, abstract no. S.114-

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