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Conference Paper: Roles of oviductal proteins on preimplantation embryo development

TitleRoles of oviductal proteins on preimplantation embryo development
Authors
KeywordsBiology
Issue Date2009
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
The 42nd Annual Meeting of the Society for the Study of Reproduction (SSR 2009), Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, 2009, v. 81 meeting abstracts, abstract no. 58 How to Cite?
AbstractSomatic cell-embryo coculture enhances embryo development invitro by producing embryotrophic factor(s) and/or removing harmfulsubstances from the culture environment. Accumulating evidencesuggested that embryos interact with the female reproductivetract to modulate the microenvironment conducive to successfulembryo development. Yet, the underlying molecular mechanismson how somatic cells interact with embryos remain largely unknown.By using suppression subtractive hybridization, we identifieda number of mouse oviductal genes that were up-regulated inthe presence of developing preimplantation embryos but not oocytes.These up-regulated genes were found to be highly expressed inthe oviduct. Some of them were also expressed in other tissuesas well. Interestingly, at least the expression of three ofthem, complement component-3 (C3), demilune parotid protein(Dcpp) and microsomal epoxide hydrolase (mEH or Ephx1), wereincreased/maintained at high levels in the oviduct during pregnancy,and were partly regulated by steroid hormones. Oviductal C3and Dcpp were embryotrophic in vitro, and Ephx1 was able toremove reactive oxygen species present in the co-culture medium.Their embryotrophic effects could be nullified by addition ofeither neutralizing antibody or specific inhibitors. Our resultssupport the notion that somatic cell coculture may enhance embryodevelopment partly via enhancing embryo developmental potentialand/or removing deleterious substances in the environment.
DescriptionSession - Minisymposium VI: MS.58
Persistent Identifierhttp://hdl.handle.net/10722/126777
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646

 

DC FieldValueLanguage
dc.contributor.authorLee, KFen_HK
dc.contributor.authorLee, YLen_HK
dc.contributor.authorCheong, AWYen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-10-31T12:47:53Z-
dc.date.available2010-10-31T12:47:53Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 42nd Annual Meeting of the Society for the Study of Reproduction (SSR 2009), Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, 2009, v. 81 meeting abstracts, abstract no. 58en_HK
dc.identifier.issn0006-3363-
dc.identifier.urihttp://hdl.handle.net/10722/126777-
dc.descriptionSession - Minisymposium VI: MS.58-
dc.description.abstractSomatic cell-embryo coculture enhances embryo development invitro by producing embryotrophic factor(s) and/or removing harmfulsubstances from the culture environment. Accumulating evidencesuggested that embryos interact with the female reproductivetract to modulate the microenvironment conducive to successfulembryo development. Yet, the underlying molecular mechanismson how somatic cells interact with embryos remain largely unknown.By using suppression subtractive hybridization, we identifieda number of mouse oviductal genes that were up-regulated inthe presence of developing preimplantation embryos but not oocytes.These up-regulated genes were found to be highly expressed inthe oviduct. Some of them were also expressed in other tissuesas well. Interestingly, at least the expression of three ofthem, complement component-3 (C3), demilune parotid protein(Dcpp) and microsomal epoxide hydrolase (mEH or Ephx1), wereincreased/maintained at high levels in the oviduct during pregnancy,and were partly regulated by steroid hormones. Oviductal C3and Dcpp were embryotrophic in vitro, and Ephx1 was able toremove reactive oxygen species present in the co-culture medium.Their embryotrophic effects could be nullified by addition ofeither neutralizing antibody or specific inhibitors. Our resultssupport the notion that somatic cell coculture may enhance embryodevelopment partly via enhancing embryo developmental potentialand/or removing deleterious substances in the environment.-
dc.languageengen_HK
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/-
dc.relation.ispartofBiology of Reproduction-
dc.subjectBiology-
dc.titleRoles of oviductal proteins on preimplantation embryo developmenten_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3363&volume=81&issue=Meeting Abstracts 58&spage=&epage=&date=2009&atitle=Roles+of+oviductal+proteins+on+preimplantation+embryo+development-
dc.identifier.emailLee, KF: ckflee@hkucc.hku.hken_HK
dc.identifier.emailLee, YL: cherielee@hkusua.hku.hken_HK
dc.identifier.emailCheong, AWY: h0343440@hkusua.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hk-
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.hkuros173366en_HK
dc.identifier.volume81-
dc.identifier.issuemeeting abstracts-
dc.description.otherThe 42nd Annual Meeting of the Society for the Study of Reproduction (SSR 2009), Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, 2009, v. 81 meeting abstracts, abstract no. 58-

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