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Conference Paper: Paracrine regulation of the production of embryotrophic oviductal complement componenet-3 and the expression of embryonic complement receptor-3 in a human oviductal cells-mouse embryo coculture system

TitleParacrine regulation of the production of embryotrophic oviductal complement componenet-3 and the expression of embryonic complement receptor-3 in a human oviductal cells-mouse embryo coculture system
Authors
KeywordsBiology
Issue Date2009
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
The 42nd Annual Meeting of the Society for the Study of Reproduction (SSR 2009), Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, 2009, v. 81 meeting abstracts, abstract no. 312 How to Cite?
AbstractThe oviduct secretes embryotrophic proteins. Human oviductalepithelial cells express complement component-3 (C3) protein and its derivatives (Lee et al., 2004). Among the C3 and derivatives,iC3b was most potent in stimulating mouse embryo development in vitro. The production of oviductal iC3b is higher in pregnantthan pseudopregnant mice (Lee et al., 2009). To further unveilthe embryotrophic action of iC3b, the regulation of oviductalC3 production and the expression of potential iC3b receptor, complement receptor-3 (CR3), in mouse embryos were studied. Mouse embryos at 2-cell and 3-4 cell stages were cocultured with primary human oviductal epithelial cells (OE) for 48 hours. The expression of C3 in the OE cells, and the components ofCR3: CD18 and CD11b, in the mouse embryos were studied. Co-culturing with mouse embryos stimulated the production of C3 mRNA in OE cells. Embryos at 3-4 cell stage were more potent than those at 2-cell stage in imposing the stimulatory effect. Immunocytochemicalstaining demonstrated stronger CD18 than CD11b signals in mouseembryos after coculturing with OE cells. The cocultured mouseembryos expressed higher levels of CD18 than CD11b transcriptswhen compared to embryos cultured in medium alone. The oviductalcells and the preimplantation embryos interact to regulate the production of oviductal C3 and embryonic iC3b receptor.
DescriptionPoster Session A: P312
Persistent Identifierhttp://hdl.handle.net/10722/126769
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646

 

DC FieldValueLanguage
dc.contributor.authorCheong, Aen_HK
dc.contributor.authorLee, YLen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorLee, KFen_HK
dc.date.accessioned2010-10-31T12:47:24Z-
dc.date.available2010-10-31T12:47:24Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 42nd Annual Meeting of the Society for the Study of Reproduction (SSR 2009), Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, 2009, v. 81 meeting abstracts, abstract no. 312en_HK
dc.identifier.issn0006-3363-
dc.identifier.urihttp://hdl.handle.net/10722/126769-
dc.descriptionPoster Session A: P312-
dc.description.abstractThe oviduct secretes embryotrophic proteins. Human oviductalepithelial cells express complement component-3 (C3) protein and its derivatives (Lee et al., 2004). Among the C3 and derivatives,iC3b was most potent in stimulating mouse embryo development in vitro. The production of oviductal iC3b is higher in pregnantthan pseudopregnant mice (Lee et al., 2009). To further unveilthe embryotrophic action of iC3b, the regulation of oviductalC3 production and the expression of potential iC3b receptor, complement receptor-3 (CR3), in mouse embryos were studied. Mouse embryos at 2-cell and 3-4 cell stages were cocultured with primary human oviductal epithelial cells (OE) for 48 hours. The expression of C3 in the OE cells, and the components ofCR3: CD18 and CD11b, in the mouse embryos were studied. Co-culturing with mouse embryos stimulated the production of C3 mRNA in OE cells. Embryos at 3-4 cell stage were more potent than those at 2-cell stage in imposing the stimulatory effect. Immunocytochemicalstaining demonstrated stronger CD18 than CD11b signals in mouseembryos after coculturing with OE cells. The cocultured mouseembryos expressed higher levels of CD18 than CD11b transcriptswhen compared to embryos cultured in medium alone. The oviductalcells and the preimplantation embryos interact to regulate the production of oviductal C3 and embryonic iC3b receptor.-
dc.languageengen_HK
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/-
dc.relation.ispartofBiology of Reproduction-
dc.subjectBiology-
dc.titleParacrine regulation of the production of embryotrophic oviductal complement componenet-3 and the expression of embryonic complement receptor-3 in a human oviductal cells-mouse embryo coculture systemen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3363&volume=81&issue=Meeting Abstracts 312&spage=&epage=&date=2009&atitle=Paracrine+regulation+of+the+production+of+embryotrophic+oviductal+complement+componenet-3+and+the+expression+of+embryonic+complement+receptor-3+in+a+human+oviductal+cells-mouse+embryo+coculture+system-
dc.identifier.emailCheong, A: h0343440@hkusua.hku.hken_HK
dc.identifier.emailLee, YL: cherielee@hkusua.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hkucc.hku.hk-
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.hkuros173367en_HK
dc.identifier.volume81-
dc.identifier.issuemeeting abstracts-
dc.description.otherThe 42nd Annual Meeting of the Society for the Study of Reproduction (SSR 2009), Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, 2009, v. 81 meeting abstracts, abstract no. 312-

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