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Conference Paper: Correlation between the nano-structure and macro-mechanics of human nucleus pulposus
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TitleCorrelation between the nano-structure and macro-mechanics of human nucleus pulposus
 
AuthorsLu, WW
Aladin, DMK
Cheung, KMC
Ngan, AHW
Chan, D
Leung, VYL
Lim, CT
Luk, KDK
 
Issue Date2009
 
CitationThe 6th SICOT/SIROT Annual International Conference, combined meeting with the Royal College of Orthopaedic Surgeons of Thailand (RCOST), Pattaya, Thailand, 29 October-1 November 2009. [How to Cite?]
 
AbstractCollagen fibrils are the main structural components of the intervertebral discs. The role played by the morphology of collagen fibrils in maintaining the mechanical integrity of the nucleus pulposus is not clear. This study aimed at quantifying the diameter of the individual collagen fibrils of the nucleus pulposus and evaluating its correlation with the bulk mechanical properties of the nucleus pulposus. Collagen fibrils were extracted from the nucleus pulposus of discs (n=7) retrieved from adolescents during scoliosis correction surgery and the extracts were confirmed by SDS-PAGE. The diameter of the individual collagen fibrils were measured through Atomic Force Microscope imaging and the compressive mechanical properties of the tissues were evaluated by confined compression. The correlations between the morphology of the collagen fibrils and the mechanical properties of the tissues were evaluated. The SDSPAGE results showed that the collagen extracts were composed of collagen II. The mean diameter of the collagen fibrils was 92.09±26.5 nm; the mean swelling pressure and compressive modulus of the tissues were 6.15±4.3 KPa and 1.23±0.7 MPa respectively. The mean fibril diameter had no linear correlation (R^2 =0. 295) with the swelling pressure of the tissues. However, it had a significant linear correlation with the compressive modulus (p=0.023, R^2=0.676). This is the first study, to our knowledge, to evaluate the nano-scale properties of the individual collagen fibrils of the disc and their relationship with macro-scale mechanical properties of the disc tissues.
 
DescriptionOral Presentations: Session: Degenerative diseases-Spine. Abstract no. 21566 - http://www.sicot.org/resources/File/Pattaya/abstract%20cdrom_oral.pdf
 
DC FieldValue
dc.contributor.authorLu, WW
 
dc.contributor.authorAladin, DMK
 
dc.contributor.authorCheung, KMC
 
dc.contributor.authorNgan, AHW
 
dc.contributor.authorChan, D
 
dc.contributor.authorLeung, VYL
 
dc.contributor.authorLim, CT
 
dc.contributor.authorLuk, KDK
 
dc.date.accessioned2010-10-31T12:35:27Z
 
dc.date.available2010-10-31T12:35:27Z
 
dc.date.issued2009
 
dc.description.abstractCollagen fibrils are the main structural components of the intervertebral discs. The role played by the morphology of collagen fibrils in maintaining the mechanical integrity of the nucleus pulposus is not clear. This study aimed at quantifying the diameter of the individual collagen fibrils of the nucleus pulposus and evaluating its correlation with the bulk mechanical properties of the nucleus pulposus. Collagen fibrils were extracted from the nucleus pulposus of discs (n=7) retrieved from adolescents during scoliosis correction surgery and the extracts were confirmed by SDS-PAGE. The diameter of the individual collagen fibrils were measured through Atomic Force Microscope imaging and the compressive mechanical properties of the tissues were evaluated by confined compression. The correlations between the morphology of the collagen fibrils and the mechanical properties of the tissues were evaluated. The SDSPAGE results showed that the collagen extracts were composed of collagen II. The mean diameter of the collagen fibrils was 92.09±26.5 nm; the mean swelling pressure and compressive modulus of the tissues were 6.15±4.3 KPa and 1.23±0.7 MPa respectively. The mean fibril diameter had no linear correlation (R^2 =0. 295) with the swelling pressure of the tissues. However, it had a significant linear correlation with the compressive modulus (p=0.023, R^2=0.676). This is the first study, to our knowledge, to evaluate the nano-scale properties of the individual collagen fibrils of the disc and their relationship with macro-scale mechanical properties of the disc tissues.
 
dc.descriptionOral Presentations: Session: Degenerative diseases-Spine. Abstract no. 21566 - http://www.sicot.org/resources/File/Pattaya/abstract%20cdrom_oral.pdf
 
dc.description.otherThe 6th SICOT/SIROT Annual International Conference, combined meeting with the Royal College of Orthopaedic Surgeons of Thailand (RCOST), Pattaya, Thailand, 29 October-1 November 2009.
 
dc.identifier.citationThe 6th SICOT/SIROT Annual International Conference, combined meeting with the Royal College of Orthopaedic Surgeons of Thailand (RCOST), Pattaya, Thailand, 29 October-1 November 2009. [How to Cite?]
 
dc.identifier.hkuros180231
 
dc.identifier.urihttp://hdl.handle.net/10722/126557
 
dc.languageeng
 
dc.relation.ispartofCombined SICOT/RCOST Annual Meeting
 
dc.titleCorrelation between the nano-structure and macro-mechanics of human nucleus pulposus
 
dc.typeConference_Paper
 
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<item><contributor.author>Lu, WW</contributor.author>
<contributor.author>Aladin, DMK</contributor.author>
<contributor.author>Cheung, KMC</contributor.author>
<contributor.author>Ngan, AHW</contributor.author>
<contributor.author>Chan, D</contributor.author>
<contributor.author>Leung, VYL</contributor.author>
<contributor.author>Lim, CT</contributor.author>
<contributor.author>Luk, KDK</contributor.author>
<date.accessioned>2010-10-31T12:35:27Z</date.accessioned>
<date.available>2010-10-31T12:35:27Z</date.available>
<date.issued>2009</date.issued>
<identifier.citation>The 6th SICOT/SIROT Annual International Conference, combined meeting with the Royal College of Orthopaedic Surgeons of Thailand (RCOST), Pattaya, Thailand, 29 October-1 November 2009.</identifier.citation>
<identifier.uri>http://hdl.handle.net/10722/126557</identifier.uri>
<description>Oral Presentations: Session: Degenerative diseases-Spine. Abstract no. 21566 - http://www.sicot.org/resources/File/Pattaya/abstract%20cdrom_oral.pdf</description>
<description.abstract>Collagen fibrils are the main structural components of the intervertebral discs. The role played by the morphology of collagen fibrils in maintaining the mechanical integrity of the nucleus pulposus is not clear. This study aimed at quantifying the diameter of the individual collagen fibrils of the nucleus pulposus and evaluating its correlation with the bulk mechanical properties of the nucleus pulposus. Collagen fibrils were extracted from the nucleus pulposus of discs (n=7) retrieved from adolescents during scoliosis correction surgery and the extracts were confirmed by SDS-PAGE. The diameter of the individual collagen fibrils were measured through Atomic Force Microscope imaging and the compressive mechanical properties of the tissues were evaluated by confined compression. The correlations between the morphology of the collagen fibrils and the mechanical properties of the tissues were evaluated. The SDSPAGE results showed that the collagen extracts were composed of collagen II. The mean diameter of the collagen fibrils was 92.09&#177;26.5 nm; the mean swelling pressure and compressive modulus of the tissues were 6.15&#177;4.3 KPa and 1.23&#177;0.7 MPa respectively. The mean fibril diameter had no linear correlation (R^2 =0. 295) with the swelling pressure of the tissues. However, it had a significant linear correlation with the compressive modulus (p=0.023, R^2=0.676). This is the first study, to our knowledge, to evaluate the nano-scale properties of the individual collagen fibrils of the disc and their relationship with macro-scale mechanical properties of the disc tissues.</description.abstract>
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<relation.ispartof>Combined SICOT/RCOST Annual Meeting</relation.ispartof>
<title>Correlation between the nano-structure and macro-mechanics of human nucleus pulposus</title>
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