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Conference Paper: Establishment of a non-transgenic mouse model for chronic hepatitisB virus infection

TitleEstablishment of a non-transgenic mouse model for chronic hepatitisB virus infection
Authors
Issue Date2010
PublisherBlackwell Publishing.
Citation
The 20th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 20), Vienna, Austria, 10-13 April 2010. How to Cite?
AbstractOBJECTIVES: Chronic hepatitisB virus (HBV) infection is a severe worldwide public health problem. Although HBV transgenic mice have been developed to mimic the immune tolerant state of chronic HBV infection, lack of a non-transgenic mouse model make it difficult, to address what happen in the early phase of infection and how the outcome of viral infection (to be eliminated or persistent) is determined. This study aimed to develop a chronic HBV infection mouse model using a humanized mouse strain with HLA-transgenic H-2 knockout background (MHC-TgKO mouse). METHODS: 20 ug of pAAV/HBV1.2, a plasmid encoding 1.2-fold of HBV genome, was delivered into mouse tail rein by hydrodynamic injection. HBsAg, HBeAg and HBV DNA were monitored in one-week intervals during a period of over 6 months. Early immune responses to HBV antigens were also determined. RESULTS: The results showed that, over 60% of injected MHC-TgKO mice persistently produced HBsAg and HBV DNA for over 6 months, which met the criteria of chronic HBV infection in humans. HBV replicative intermediates and transcripts were exclusively detected in the liver carrier mice, but not in other organs. Similar to that in human, injected female mice had lower HBsAg persistent rate, HBsAg concentration and HBV DNA levels than the male. A significant lower immune response to HBcAg was detected in MHC-TgKO mice than that in C57BL/6 by ELISPOT assay, which may explain the high persistent rate in MHC-TgKO mice. CONCLUSION: This study has established a mouse model for chronic HBV infection, and further investigations are on going to characterize the virological and immunological properties in carrier mice.
DescriptionAbstract no. P1141
Persistent Identifierhttp://hdl.handle.net/10722/126461

 

DC FieldValueLanguage
dc.contributor.authorYang, Len_HK
dc.contributor.authorZheng, Ben_HK
dc.date.accessioned2010-10-31T12:30:00Z-
dc.date.available2010-10-31T12:30:00Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 20th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 20), Vienna, Austria, 10-13 April 2010.en_HK
dc.identifier.urihttp://hdl.handle.net/10722/126461-
dc.descriptionAbstract no. P1141-
dc.description.abstractOBJECTIVES: Chronic hepatitisB virus (HBV) infection is a severe worldwide public health problem. Although HBV transgenic mice have been developed to mimic the immune tolerant state of chronic HBV infection, lack of a non-transgenic mouse model make it difficult, to address what happen in the early phase of infection and how the outcome of viral infection (to be eliminated or persistent) is determined. This study aimed to develop a chronic HBV infection mouse model using a humanized mouse strain with HLA-transgenic H-2 knockout background (MHC-TgKO mouse). METHODS: 20 ug of pAAV/HBV1.2, a plasmid encoding 1.2-fold of HBV genome, was delivered into mouse tail rein by hydrodynamic injection. HBsAg, HBeAg and HBV DNA were monitored in one-week intervals during a period of over 6 months. Early immune responses to HBV antigens were also determined. RESULTS: The results showed that, over 60% of injected MHC-TgKO mice persistently produced HBsAg and HBV DNA for over 6 months, which met the criteria of chronic HBV infection in humans. HBV replicative intermediates and transcripts were exclusively detected in the liver carrier mice, but not in other organs. Similar to that in human, injected female mice had lower HBsAg persistent rate, HBsAg concentration and HBV DNA levels than the male. A significant lower immune response to HBcAg was detected in MHC-TgKO mice than that in C57BL/6 by ELISPOT assay, which may explain the high persistent rate in MHC-TgKO mice. CONCLUSION: This study has established a mouse model for chronic HBV infection, and further investigations are on going to characterize the virological and immunological properties in carrier mice.-
dc.languageengen_HK
dc.publisherBlackwell Publishing.-
dc.relation.ispartofEuropean Congress of Clinical Microbiology and Infectious Diseases-
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.titleEstablishment of a non-transgenic mouse model for chronic hepatitisB virus infectionen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYang, L: yangling@hku.hken_HK
dc.identifier.emailZheng, B: bzheng@hkucc.hku.hk-
dc.identifier.authorityZheng, B=rp00353en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros175112en_HK
dc.description.otherThe 20th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 20), Vienna, Austria, 10-13 April 2010.-

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