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Conference Paper: Antibacterial effect of silver and fluoride compounds on cariogenic organisms
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TitleAntibacterial effect of silver and fluoride compounds on cariogenic organisms
 
AuthorsLou, Y
Botelho, M
Darvell, BW
 
Issue Date2010
 
PublisherInternational Association of Dental Research.
 
CitationThe IADR 2010 General Session, Barcelona, Spain, 14-17 July 2010. [How to Cite?]
 
AbstractOBJECTIVE: to investigate the antibacterial effects of silver- and fluoride-containing compounds used in caries management. METHODS: Minimum inhibitory concentrations (MICs) of silver diammine fluoride (SDF), silver fluoride (AgF), silver nitrate (AgNO3), ammonium fluoride (NH4F) and sodium fluoride (NaF) were determined on Streptococcus mutans, Lactobacillus acidophilus and Actinomyces naeslundii. Serial (×2) dilutions from 2 400,000 µg•mL-1 (limits depending on solubility) were prepared in a 96-well plate with BHI media adjusted to pH 7 or pH 5 to simulate neutral and cariogenic conditions. Tests with silver compounds were duplicated in the dark to investigate the effect of light. RESULTS: Four-way analysis of variance (AoV) showed three main factors (agent, pH, species) to have highly-significant effects (p < 0.05) while light did not (p > 0.05). Dropping light and repeating as a three-way AoV, all factors remained significant as well as most interactions (p < 0.05). MIC(SDF) on the three bacteria ranged from 62 125 µg•mL-1, similar to AgNO3 (62 µg•mL-1) and AgF (31 62 µg•mL-1). For A. naeslundii and S. mutans, silver compounds had antibacterial effect at significantly lower concentration than the fluoride compounds (p < 0.001); NH4F (313 µg•mL-1) and NaF (500 µg•mL-1) had significantly weaker (p < 0.05) effects at pH 7 than at pH 5. Increased antibacterial effect was found for F at the lower pH (p < 0.001) but not for Ag. MICs for L. acidophilus could not be determined at pH 5 due to inadequate growth, while at pH 7 there was no growth for NaF (2 µg•mL-1) or NH4F (20 µg•mL-1), implying lower MIC values. CONCLUSIONS: Silver ions are antibacterial against oral microorganisms even at low concentration, while fluoride ion was only effective at relatively high concentration and affected by pH.
 
DescriptionPoster Session: abstract no. 1624
 
DC FieldValue
dc.contributor.authorLou, Y
 
dc.contributor.authorBotelho, M
 
dc.contributor.authorDarvell, BW
 
dc.date.accessioned2010-10-31T11:49:53Z
 
dc.date.available2010-10-31T11:49:53Z
 
dc.date.issued2010
 
dc.description.abstractOBJECTIVE: to investigate the antibacterial effects of silver- and fluoride-containing compounds used in caries management. METHODS: Minimum inhibitory concentrations (MICs) of silver diammine fluoride (SDF), silver fluoride (AgF), silver nitrate (AgNO3), ammonium fluoride (NH4F) and sodium fluoride (NaF) were determined on Streptococcus mutans, Lactobacillus acidophilus and Actinomyces naeslundii. Serial (×2) dilutions from 2 400,000 µg•mL-1 (limits depending on solubility) were prepared in a 96-well plate with BHI media adjusted to pH 7 or pH 5 to simulate neutral and cariogenic conditions. Tests with silver compounds were duplicated in the dark to investigate the effect of light. RESULTS: Four-way analysis of variance (AoV) showed three main factors (agent, pH, species) to have highly-significant effects (p < 0.05) while light did not (p > 0.05). Dropping light and repeating as a three-way AoV, all factors remained significant as well as most interactions (p < 0.05). MIC(SDF) on the three bacteria ranged from 62 125 µg•mL-1, similar to AgNO3 (62 µg•mL-1) and AgF (31 62 µg•mL-1). For A. naeslundii and S. mutans, silver compounds had antibacterial effect at significantly lower concentration than the fluoride compounds (p < 0.001); NH4F (313 µg•mL-1) and NaF (500 µg•mL-1) had significantly weaker (p < 0.05) effects at pH 7 than at pH 5. Increased antibacterial effect was found for F at the lower pH (p < 0.001) but not for Ag. MICs for L. acidophilus could not be determined at pH 5 due to inadequate growth, while at pH 7 there was no growth for NaF (2 µg•mL-1) or NH4F (20 µg•mL-1), implying lower MIC values. CONCLUSIONS: Silver ions are antibacterial against oral microorganisms even at low concentration, while fluoride ion was only effective at relatively high concentration and affected by pH.
 
dc.description.naturelink_to_OA_fulltext
 
dc.descriptionPoster Session: abstract no. 1624
 
dc.description.otherThe IADR 2010 General Session, Barcelona, Spain, 14-17 July 2010.
 
dc.identifier.citationThe IADR 2010 General Session, Barcelona, Spain, 14-17 July 2010. [How to Cite?]
 
dc.identifier.hkuros180044
 
dc.identifier.urihttp://hdl.handle.net/10722/125751
 
dc.languageeng
 
dc.publisherInternational Association of Dental Research.
 
dc.publisher.placeUnited States
 
dc.relation.ispartofIADR General Session, 2010
 
dc.titleAntibacterial effect of silver and fluoride compounds on cariogenic organisms
 
dc.typeConference_Paper
 
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<item><contributor.author>Lou, Y</contributor.author>
<contributor.author>Botelho, M</contributor.author>
<contributor.author>Darvell, BW</contributor.author>
<date.accessioned>2010-10-31T11:49:53Z</date.accessioned>
<date.available>2010-10-31T11:49:53Z</date.available>
<date.issued>2010</date.issued>
<identifier.citation>The IADR 2010 General Session, Barcelona, Spain, 14-17 July 2010.</identifier.citation>
<identifier.uri>http://hdl.handle.net/10722/125751</identifier.uri>
<description>Poster Session: abstract no. 1624</description>
<description.abstract>OBJECTIVE: to investigate the antibacterial effects of silver- and fluoride-containing compounds used in caries management. METHODS: Minimum inhibitory concentrations (MICs) of silver diammine fluoride (SDF), silver fluoride (AgF), silver nitrate (AgNO3), ammonium fluoride (NH4F) and sodium fluoride (NaF) were determined on Streptococcus mutans, Lactobacillus acidophilus and Actinomyces naeslundii. Serial (&#215;2) dilutions from 2  400,000 &#181;g&#8226;mL-1 (limits depending on solubility) were prepared in a 96-well plate with BHI media adjusted to pH 7 or pH 5 to simulate neutral and cariogenic conditions. Tests with silver compounds were duplicated in the dark to investigate the effect of light. RESULTS: Four-way analysis of variance (AoV) showed three main factors (agent, pH, species) to have highly-significant effects (p &lt; 0.05) while light did not (p &gt; 0.05). Dropping light and repeating as a three-way AoV, all factors remained significant as well as most interactions (p &lt; 0.05). MIC(SDF) on the three bacteria ranged from 62  125 &#181;g&#8226;mL-1, similar to AgNO3 (62 &#181;g&#8226;mL-1) and AgF (31  62 &#181;g&#8226;mL-1). For A. naeslundii and S. mutans, silver compounds had antibacterial effect at significantly lower concentration than the fluoride compounds (p &lt; 0.001); NH4F (313 &#181;g&#8226;mL-1) and NaF (500 &#181;g&#8226;mL-1) had significantly weaker (p &lt; 0.05) effects at pH 7 than at pH 5. Increased antibacterial effect was found for F at the lower pH (p &lt; 0.001) but not for Ag. MICs for L. acidophilus could not be determined at pH 5 due to inadequate growth, while at pH 7 there was no growth for NaF (2 &#181;g&#8226;mL-1) or NH4F (20 &#181;g&#8226;mL-1), implying lower MIC values. CONCLUSIONS: Silver ions are antibacterial against oral microorganisms even at low concentration, while fluoride ion was only effective at relatively high concentration and affected by pH.</description.abstract>
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