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Article: One time intranasal vaccination with a modified vaccinia Tiantan strain MVTT ZCI protects animals against pathogenic viral challenge

TitleOne time intranasal vaccination with a modified vaccinia Tiantan strain MVTT ZCI protects animals against pathogenic viral challenge
Authors
KeywordsMucosal vaccine
MVTT ZCI
Smallpox
Tiantan
Vaccinia
Issue Date2010
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
Citation
Vaccine, 2010, v. 28 n. 9, p. 2088-2096 How to Cite?
AbstractTo combat variola virus in bioterrorist attacks, it is desirable to develop a noninvasive vaccine. Based on the vaccinia Tiantan (VTT) strain, which was historically used to eradicate the smallpox in China, we generated a modified VTT (MVTT ZCI) by removing the hemagglutinin gene and an 11,944 bp genomic region from HindIII fragment C2L to F3L. MVTT ZCI was characterized for its host cell range in vitro and preclinical safety and efficacy profiles in mice. Despite replication-competency in some cell lines, unlike VTT, MVTT ZCI did not cause death after intracranial injection or body weight loss after intranasal inoculation. MVTT ZCI did not replicate in mouse brain and was safe in immunodeficient mice. MVTT ZCI induced neutralizing antibodies via the intranasal route of immunization. One time intranasal immunization protected animals from the challenge of the pathogenic vaccinia WR strain. This study established proof-of-concept that the attenuated replicating MVTT ZCI may serve as a safe noninvasive smallpox vaccine candidate. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/125558
ISSN
2015 Impact Factor: 3.413
2015 SCImago Journal Rankings: 2.044
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong research grant councilHK-RGC762208
HK-RGC762209
National Basic Research Program of China2006CB504208
National 11th 5-year project2008ZX10001-011
University Development Fund of the University of Hong Kong
Funding Information:

This work was supported by Hong Kong research grant council (HK-RGC762208 and HK-RGC762209 to ZC), the National Basic Research Program of China (the 973 project 2006CB504208), the National 11th 5-year project 2008ZX10001-011 and the University Development Fund of the University of Hong Kong to its AIDS Institute. We thank Drs. D. Ho and K.Y. Yuen for scientific advice and Dr. M. Mackett for critical reading of the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorYu, Wen_HK
dc.contributor.authorFang, Qen_HK
dc.contributor.authorZhu, Wen_HK
dc.contributor.authorWang, Hen_HK
dc.contributor.authorTien, Pen_HK
dc.contributor.authorZhang, Len_HK
dc.contributor.authorChen, Zen_HK
dc.date.accessioned2010-10-31T11:38:12Z-
dc.date.available2010-10-31T11:38:12Z-
dc.date.issued2010en_HK
dc.identifier.citationVaccine, 2010, v. 28 n. 9, p. 2088-2096en_HK
dc.identifier.issn0264-410Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/125558-
dc.description.abstractTo combat variola virus in bioterrorist attacks, it is desirable to develop a noninvasive vaccine. Based on the vaccinia Tiantan (VTT) strain, which was historically used to eradicate the smallpox in China, we generated a modified VTT (MVTT ZCI) by removing the hemagglutinin gene and an 11,944 bp genomic region from HindIII fragment C2L to F3L. MVTT ZCI was characterized for its host cell range in vitro and preclinical safety and efficacy profiles in mice. Despite replication-competency in some cell lines, unlike VTT, MVTT ZCI did not cause death after intracranial injection or body weight loss after intranasal inoculation. MVTT ZCI did not replicate in mouse brain and was safe in immunodeficient mice. MVTT ZCI induced neutralizing antibodies via the intranasal route of immunization. One time intranasal immunization protected animals from the challenge of the pathogenic vaccinia WR strain. This study established proof-of-concept that the attenuated replicating MVTT ZCI may serve as a safe noninvasive smallpox vaccine candidate. © 2009 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccineen_HK
dc.relation.ispartofVaccineen_HK
dc.subjectMucosal vaccineen_HK
dc.subjectMVTT ZCIen_HK
dc.subjectSmallpoxen_HK
dc.subjectTiantanen_HK
dc.subjectVacciniaen_HK
dc.subject.meshAdministration, Intranasal-
dc.subject.meshAntibodies, Neutralizing - immunology-
dc.subject.meshSmallpox - immunology - prevention and control-
dc.subject.meshSmallpox Vaccine - administration and dosage - biosynthesis - genetics - immunology-
dc.subject.meshVaccinia virus - genetics - immunology - pathogenicity-
dc.titleOne time intranasal vaccination with a modified vaccinia Tiantan strain MVTT ZCI protects animals against pathogenic viral challengeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0264-410X&volume=28&issue=9&spage=2088&epage=2096&date=2010&atitle=One+time+intranasal+vaccination+with+a+modified+vaccinia+Tiantan+strain+MVTT+ZCI+protects+animals+against+pathogenic+viral+challenge-
dc.identifier.emailWang, H: haibo@hku.hken_HK
dc.identifier.emailChen, Z: zchenai@hkucc.hku.hken_HK
dc.identifier.authorityWang, H=rp00279en_HK
dc.identifier.authorityChen, Z=rp00243en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.vaccine.2009.12.038en_HK
dc.identifier.pmid20045097-
dc.identifier.scopuseid_2-s2.0-77149177048en_HK
dc.identifier.hkuros181672en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77149177048&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue9en_HK
dc.identifier.spage2088en_HK
dc.identifier.epage2096en_HK
dc.identifier.isiWOS:000275991300011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYu, W=50162901500en_HK
dc.identifier.scopusauthoridFang, Q=55248545600en_HK
dc.identifier.scopusauthoridZhu, W=8377176500en_HK
dc.identifier.scopusauthoridWang, H=36143443600en_HK
dc.identifier.scopusauthoridTien, P=7006421622en_HK
dc.identifier.scopusauthoridZhang, L=8783285300en_HK
dc.identifier.scopusauthoridChen, Z=35271180800en_HK
dc.identifier.citeulike6482456-

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