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Article: Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus

TitleAngiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus
Authors
Issue Date2009
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal Of General Virology, 2009, v. 90 n. 11, p. 2695-2703 How to Cite?
AbstractRaccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has identities of 99.3, 89.2, 83.9 and 80.4% to ACE2 proteins from dog, masked palm civet (pcACE2), human (huACE2) and bat, respectively. There are six amino acid changes in rdACE2 compared with huACE2, and four changes compared with pcACE2, within the 18 residues of ACE2 known to make direct contact with the SARS-CoV S protein. A HeLa cell line stably expressing rdACE2 was established; Western blot analyses and an enzyme-activity assay indicated that the cell line expressed ACE2 at a similar level to two previously established cell lines that express ACE2 from human and masked palm civet, respectively. Human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human SARS-CoV or SARS-CoV-like viruses of masked palm civets and raccoon dogs were tested for their entry efficiency into these cell lines. The results showed that rdACE2 is a more efficient receptor for human SARS-CoV, but not for SARS-CoV-like viruses of masked palm civets and raccoon dogs, than huACE2 or pcACE2. This study provides useful data to elucidate the role of raccoon dog in SARS outbreaks. © 2009 SGM.
Persistent Identifierhttp://hdl.handle.net/10722/125546
ISSN
2015 Impact Factor: 3.192
2015 SCImago Journal Rankings: 1.741
ISI Accession Number ID
Funding AgencyGrant Number
MOST2003CB514118
2005CB523004
EU FP6SP22-CT-2004-511060
EPISARSSP22-CT-2004-511603
Funding Information:

This work was supported by MOST projects (2003CB514118, 2005CB523004 and a special grant for `Animal Reservoir of SARS-CoV'), and by EU FP6 projects DISSECT (no. SP22-CT-2004-511060) and EPISARS (no. SP22-CT-2004-511603). We thank Dr Basil M. Arif for scientific editing of the manuscript. We also thank Dr Zhengli Shi (Wuhan Institute of Virology, CAS) for providing the pVPack pseudovirus system and Dr Linfa Wang (Australian Animal Health Laboratory, CSIRO) for providing QFS and for helpful discussions.

References

 

DC FieldValueLanguage
dc.contributor.authorXu, Len_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorChen, Zen_HK
dc.contributor.authorDeng, Hen_HK
dc.contributor.authorMa, Zen_HK
dc.contributor.authorWang, Hen_HK
dc.contributor.authorHu, Zen_HK
dc.contributor.authorDeng, Fen_HK
dc.date.accessioned2010-10-31T11:37:31Z-
dc.date.available2010-10-31T11:37:31Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of General Virology, 2009, v. 90 n. 11, p. 2695-2703en_HK
dc.identifier.issn0022-1317en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125546-
dc.description.abstractRaccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has identities of 99.3, 89.2, 83.9 and 80.4% to ACE2 proteins from dog, masked palm civet (pcACE2), human (huACE2) and bat, respectively. There are six amino acid changes in rdACE2 compared with huACE2, and four changes compared with pcACE2, within the 18 residues of ACE2 known to make direct contact with the SARS-CoV S protein. A HeLa cell line stably expressing rdACE2 was established; Western blot analyses and an enzyme-activity assay indicated that the cell line expressed ACE2 at a similar level to two previously established cell lines that express ACE2 from human and masked palm civet, respectively. Human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human SARS-CoV or SARS-CoV-like viruses of masked palm civets and raccoon dogs were tested for their entry efficiency into these cell lines. The results showed that rdACE2 is a more efficient receptor for human SARS-CoV, but not for SARS-CoV-like viruses of masked palm civets and raccoon dogs, than huACE2 or pcACE2. This study provides useful data to elucidate the role of raccoon dog in SARS outbreaks. © 2009 SGM.en_HK
dc.languageengen_HK
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_HK
dc.relation.ispartofJournal of General Virologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of General Virology. Copyright © Society for General Microbiology.-
dc.rightsThis is an author manuscript that has been accepted for publication in [Journal Title], copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in [Journal Title]. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at [Journal URL], and is freely available without a subscription.-
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMembrane Glycoproteins - metabolismen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPeptidyl-Dipeptidase A - genetics - metabolismen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshProtein Bindingen_HK
dc.subject.meshRaccoon Dogsen_HK
dc.subject.meshReceptors, Virus - metabolismen_HK
dc.subject.meshSARS Virus - physiologyen_HK
dc.subject.meshSequence Alignmenten_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSequence Homology, Amino Aciden_HK
dc.subject.meshViral Envelope Proteins - metabolismen_HK
dc.subject.meshVirus Attachmenten_HK
dc.subject.meshVirus Internalizationen_HK
dc.titleAngiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1317&volume=90&spage=2695&epage=2703&date=2009&atitle=Angiotensin-converting+Enzyme+2+(ACE2)+From+Raccoon+Dog+Can+Serve+As+An+Efficient+Receptor+For+The+Spike+Protein+Of+Severe+Acute+Respiratory+Syndrome+Coronavirus-
dc.identifier.emailChen, Z:zchenai@hkucc.hku.hken_HK
dc.identifier.authorityChen, Z=rp00243en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1099/vir.0.013490-0en_HK
dc.identifier.pmid19625462-
dc.identifier.scopuseid_2-s2.0-70350464988en_HK
dc.identifier.hkuros181508en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70350464988&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume90en_HK
dc.identifier.issue11en_HK
dc.identifier.spage2695en_HK
dc.identifier.epage2703en_HK
dc.identifier.isiWOS:000271440500015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridXu, L=26432885300en_HK
dc.identifier.scopusauthoridZhang, Y=24081841200en_HK
dc.identifier.scopusauthoridLiu, Y=36071856600en_HK
dc.identifier.scopusauthoridChen, Z=35271180800en_HK
dc.identifier.scopusauthoridDeng, H=7401775438en_HK
dc.identifier.scopusauthoridMa, Z=35107816400en_HK
dc.identifier.scopusauthoridWang, H=8974614500en_HK
dc.identifier.scopusauthoridHu, Z=7404210963en_HK
dc.identifier.scopusauthoridDeng, F=35847541100en_HK

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