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Article: The Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty

TitleThe Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplasty
Authors
Issue Date2010
PublisherElsevier Inc.. The Journal's web site is located at http://www.jvir.org/
Citation
Journal Of Vascular And Interventional Radiology, 2010, v. 21 n. 6, p. 910-915 How to Cite?
AbstractPurpose: To investigate the effects of supercooling, a phase whereby cells are below 0°C but still in a liquid state, and freezing, the phase when cells become solid, of vascular cells in culture. Materials and Methods: Bovine aortic endothelial cells and smooth muscle cells were supercooled to -10°C with or without freezing for 3, 30, or 60 seconds and then rewarmed to 37°C for 24 hours. Viability was assessed by means of trypan blue exclusion, and apoptosis was assessed with the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) assay. Results: Viability of smooth muscle cells decreased 49% after freezing versus supercooling (P< .05). Endothelial cells maintained greater viability rates. A 19.5% smooth muscle cell apoptotic rate was observed after freezing, whereas smooth muscle cell supercooling yielded rates of only 11% (P< .05). A 4.17% endothelial cell apoptotic rate was observed after freezing, whereas supercooled endothelial cells yielded a 1.76% rate (P< .05). Conclusions: Freezing results in decreased viability and increased apoptosis compared to supercooling in both cell lines. Smooth muscle cells appear more susceptible to freezing. The biologic effects of freezing on vascular cells may elucidate the mechanisms behind the enhanced patency after cryoplasty of atherosclerotic lesions. © 2010 SIR.
Persistent Identifierhttp://hdl.handle.net/10722/125441
ISSN
2015 Impact Factor: 2.57
2015 SCImago Journal Rankings: 1.210
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of HealthHL R01-47345
VA Merit Review Board
North American Center for Limb Preservation
Funding Information:

B.E.S. has received salary support through NIH funding. The study was funded in part by grants from the National Institutes of Health HL R01-47345, VA Merit Review Board, and the North American Center for Limb Preservation. None of the other authors have identified a conflict of interest.

References

 

DC FieldValueLanguage
dc.contributor.authorBasco, MTGen_HK
dc.contributor.authorYiu, Wken_HK
dc.contributor.authorCheng, SWKen_HK
dc.contributor.authorSumpio, BEen_HK
dc.date.accessioned2010-10-31T11:31:36Z-
dc.date.available2010-10-31T11:31:36Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Vascular And Interventional Radiology, 2010, v. 21 n. 6, p. 910-915en_HK
dc.identifier.issn1051-0443en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125441-
dc.description.abstractPurpose: To investigate the effects of supercooling, a phase whereby cells are below 0°C but still in a liquid state, and freezing, the phase when cells become solid, of vascular cells in culture. Materials and Methods: Bovine aortic endothelial cells and smooth muscle cells were supercooled to -10°C with or without freezing for 3, 30, or 60 seconds and then rewarmed to 37°C for 24 hours. Viability was assessed by means of trypan blue exclusion, and apoptosis was assessed with the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) assay. Results: Viability of smooth muscle cells decreased 49% after freezing versus supercooling (P< .05). Endothelial cells maintained greater viability rates. A 19.5% smooth muscle cell apoptotic rate was observed after freezing, whereas smooth muscle cell supercooling yielded rates of only 11% (P< .05). A 4.17% endothelial cell apoptotic rate was observed after freezing, whereas supercooled endothelial cells yielded a 1.76% rate (P< .05). Conclusions: Freezing results in decreased viability and increased apoptosis compared to supercooling in both cell lines. Smooth muscle cells appear more susceptible to freezing. The biologic effects of freezing on vascular cells may elucidate the mechanisms behind the enhanced patency after cryoplasty of atherosclerotic lesions. © 2010 SIR.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc.. The Journal's web site is located at http://www.jvir.org/en_HK
dc.relation.ispartofJournal of Vascular and Interventional Radiologyen_HK
dc.titleThe Effects of Freezing versus Supercooling on Vascular Cells: Implications for Balloon Cryoplastyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1051-0443&volume=21&issue=6&spage=910&epage=915&date=2010&atitle=The+effects+of+freezing+versus+supercooling+on+vascular+cells:+Implications+for+balloon+cryoplastyen_HK
dc.identifier.emailYiu, Wk: waikiyiu@hku.hken_HK
dc.identifier.emailCheng, SWK: wkcheng@hkucc.hku.hken_HK
dc.identifier.authorityYiu, Wk=rp00311en_HK
dc.identifier.authorityCheng, SWK=rp00374en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.jvir.2010.02.016en_HK
dc.identifier.pmid20417120-
dc.identifier.pmcidPMC2878641-
dc.identifier.scopuseid_2-s2.0-77952322434en_HK
dc.identifier.hkuros175981en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952322434&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue6en_HK
dc.identifier.spage910en_HK
dc.identifier.epage915en_HK
dc.identifier.eissn1535-7732-
dc.identifier.isiWOS:000278528100020-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridBasco, MTG=26425440300en_HK
dc.identifier.scopusauthoridYiu, Wk=12763171700en_HK
dc.identifier.scopusauthoridCheng, SWK=7404684779en_HK
dc.identifier.scopusauthoridSumpio, BE=7103201423en_HK

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