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Article: Bone marrow-derived Schwann cells achieve fate commitment - a prerequisite for remyelination therapy

TitleBone marrow-derived Schwann cells achieve fate commitment - a prerequisite for remyelination therapy
Authors
KeywordsBone marrow
Cell differentiation
Dorsal root ganglia
Myelination
Schwann cells
Issue Date2010
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnr
Citation
Experimental Neurology, 2010, v. 224 n. 2, p. 448-458 How to Cite?
AbstractSchwann cell transplantation improves post-traumatic nerve regeneration in both PNS and CNS but sufficient numbers of immunocompatible cells are required for clinical application. Currently, Schwann cell-like cells derived from the bone marrow lack fate commitment and revert to a fibroblast-like phenotype upon withdrawal of differentiation-inducing factors. In recapitulation of embryonic events leading to Schwann cell maturation, we hypothesize that the Schwann cell-like cells acquire the switch to fate commitment through contact-dependant cues from incipient neurons of the developing dorsal root ganglia. To address this, Schwann cell-like cells derived from adult rat bone marrow were cocultured with neurons purified from embryonic dorsal root ganglia. A cell-intrinsic switch to the Schwann cell fate was achieved consistently and the cell progeny maintained expression of the markers S100β, p75NTR , GFAP, P0 and Sox 10 even without exogenous differentiation-inducing factors or neurons. In vitro formation of MBP-positive segments under myelinating conditions by the cell progeny was comparable to that by sciatic nerve-derived Schwann cells. Controls in which Schwann cell-like cells were barred from direct contact with neurons in coculture reverted to SMA/CD90-expressing myofibroblasts. We demonstrate therefore for the first time fate commitment among bone marrow-derived Schwann cells. The therapeutic potential of these cells may be tested in future transplantation studies. (206 words). © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/125309
ISSN
2015 Impact Factor: 4.657
2015 SCImago Journal Rankings: 2.427
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Croucher Foundation
Funding Information:

Supported in part by funding from Strategic Research Theme of The University of Hong Kong and Croucher Foundation MBBS/PhD scholarship to GKH Shea.

References

 

DC FieldValueLanguage
dc.contributor.authorShea, GKHen_HK
dc.contributor.authorTsui, AYPen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-10-31T11:23:44Z-
dc.date.available2010-10-31T11:23:44Z-
dc.date.issued2010en_HK
dc.identifier.citationExperimental Neurology, 2010, v. 224 n. 2, p. 448-458en_HK
dc.identifier.issn0014-4886en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125309-
dc.description.abstractSchwann cell transplantation improves post-traumatic nerve regeneration in both PNS and CNS but sufficient numbers of immunocompatible cells are required for clinical application. Currently, Schwann cell-like cells derived from the bone marrow lack fate commitment and revert to a fibroblast-like phenotype upon withdrawal of differentiation-inducing factors. In recapitulation of embryonic events leading to Schwann cell maturation, we hypothesize that the Schwann cell-like cells acquire the switch to fate commitment through contact-dependant cues from incipient neurons of the developing dorsal root ganglia. To address this, Schwann cell-like cells derived from adult rat bone marrow were cocultured with neurons purified from embryonic dorsal root ganglia. A cell-intrinsic switch to the Schwann cell fate was achieved consistently and the cell progeny maintained expression of the markers S100β, p75NTR , GFAP, P0 and Sox 10 even without exogenous differentiation-inducing factors or neurons. In vitro formation of MBP-positive segments under myelinating conditions by the cell progeny was comparable to that by sciatic nerve-derived Schwann cells. Controls in which Schwann cell-like cells were barred from direct contact with neurons in coculture reverted to SMA/CD90-expressing myofibroblasts. We demonstrate therefore for the first time fate commitment among bone marrow-derived Schwann cells. The therapeutic potential of these cells may be tested in future transplantation studies. (206 words). © 2010 Elsevier Inc.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnren_HK
dc.relation.ispartofExperimental Neurologyen_HK
dc.subjectBone marrowen_HK
dc.subjectCell differentiationen_HK
dc.subjectDorsal root gangliaen_HK
dc.subjectMyelinationen_HK
dc.subjectSchwann cellsen_HK
dc.subject.meshAnimals-
dc.subject.meshBone Marrow Cells - cytology-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Lineage-
dc.subject.meshSchwann Cells - cytology-
dc.titleBone marrow-derived Schwann cells achieve fate commitment - a prerequisite for remyelination therapyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-4886&volume=224&issue=2&spage=448&epage=458&date=2010&atitle=Bone+marrow-derived+Schwann+cells+achieve+fate+commitment+–+a+prerequisite+for+remyelination+therapyen_HK
dc.identifier.emailShea, GKH: gkshea@hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityShea, GKH=rp01781en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.expneurol.2010.05.005en_HK
dc.identifier.pmid20483356-
dc.identifier.scopuseid_2-s2.0-77954658180en_HK
dc.identifier.hkuros174275en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954658180&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume224en_HK
dc.identifier.issue2en_HK
dc.identifier.spage448en_HK
dc.identifier.epage458en_HK
dc.identifier.eissn1090-2430-
dc.identifier.isiWOS:000280339600015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShea, GKH=36341273600en_HK
dc.identifier.scopusauthoridTsui, AYP=36341669100en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.citeulike7266695-

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