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- Publisher Website: 10.1016/j.expneurol.2010.05.005
- Scopus: eid_2-s2.0-77954658180
- PMID: 20483356
- WOS: WOS:000280339600015
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Article: Bone marrow-derived Schwann cells achieve fate commitment - a prerequisite for remyelination therapy
Title | Bone marrow-derived Schwann cells achieve fate commitment - a prerequisite for remyelination therapy | ||||||
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Authors | |||||||
Keywords | Bone marrow Cell differentiation Dorsal root ganglia Myelination Schwann cells | ||||||
Issue Date | 2010 | ||||||
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnr | ||||||
Citation | Experimental Neurology, 2010, v. 224 n. 2, p. 448-458 How to Cite? | ||||||
Abstract | Schwann cell transplantation improves post-traumatic nerve regeneration in both PNS and CNS but sufficient numbers of immunocompatible cells are required for clinical application. Currently, Schwann cell-like cells derived from the bone marrow lack fate commitment and revert to a fibroblast-like phenotype upon withdrawal of differentiation-inducing factors. In recapitulation of embryonic events leading to Schwann cell maturation, we hypothesize that the Schwann cell-like cells acquire the switch to fate commitment through contact-dependant cues from incipient neurons of the developing dorsal root ganglia. To address this, Schwann cell-like cells derived from adult rat bone marrow were cocultured with neurons purified from embryonic dorsal root ganglia. A cell-intrinsic switch to the Schwann cell fate was achieved consistently and the cell progeny maintained expression of the markers S100β, p75NTR , GFAP, P0 and Sox 10 even without exogenous differentiation-inducing factors or neurons. In vitro formation of MBP-positive segments under myelinating conditions by the cell progeny was comparable to that by sciatic nerve-derived Schwann cells. Controls in which Schwann cell-like cells were barred from direct contact with neurons in coculture reverted to SMA/CD90-expressing myofibroblasts. We demonstrate therefore for the first time fate commitment among bone marrow-derived Schwann cells. The therapeutic potential of these cells may be tested in future transplantation studies. (206 words). © 2010 Elsevier Inc. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/125309 | ||||||
ISSN | 2023 Impact Factor: 4.6 2023 SCImago Journal Rankings: 1.552 | ||||||
ISI Accession Number ID |
Funding Information: Supported in part by funding from Strategic Research Theme of The University of Hong Kong and Croucher Foundation MBBS/PhD scholarship to GKH Shea. | ||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shea, GKH | en_HK |
dc.contributor.author | Tsui, AYP | en_HK |
dc.contributor.author | Chan, YS | en_HK |
dc.contributor.author | Shum, DKY | en_HK |
dc.date.accessioned | 2010-10-31T11:23:44Z | - |
dc.date.available | 2010-10-31T11:23:44Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | Experimental Neurology, 2010, v. 224 n. 2, p. 448-458 | en_HK |
dc.identifier.issn | 0014-4886 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/125309 | - |
dc.description.abstract | Schwann cell transplantation improves post-traumatic nerve regeneration in both PNS and CNS but sufficient numbers of immunocompatible cells are required for clinical application. Currently, Schwann cell-like cells derived from the bone marrow lack fate commitment and revert to a fibroblast-like phenotype upon withdrawal of differentiation-inducing factors. In recapitulation of embryonic events leading to Schwann cell maturation, we hypothesize that the Schwann cell-like cells acquire the switch to fate commitment through contact-dependant cues from incipient neurons of the developing dorsal root ganglia. To address this, Schwann cell-like cells derived from adult rat bone marrow were cocultured with neurons purified from embryonic dorsal root ganglia. A cell-intrinsic switch to the Schwann cell fate was achieved consistently and the cell progeny maintained expression of the markers S100β, p75NTR , GFAP, P0 and Sox 10 even without exogenous differentiation-inducing factors or neurons. In vitro formation of MBP-positive segments under myelinating conditions by the cell progeny was comparable to that by sciatic nerve-derived Schwann cells. Controls in which Schwann cell-like cells were barred from direct contact with neurons in coculture reverted to SMA/CD90-expressing myofibroblasts. We demonstrate therefore for the first time fate commitment among bone marrow-derived Schwann cells. The therapeutic potential of these cells may be tested in future transplantation studies. (206 words). © 2010 Elsevier Inc. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnr | en_HK |
dc.relation.ispartof | Experimental Neurology | en_HK |
dc.subject | Bone marrow | en_HK |
dc.subject | Cell differentiation | en_HK |
dc.subject | Dorsal root ganglia | en_HK |
dc.subject | Myelination | en_HK |
dc.subject | Schwann cells | en_HK |
dc.subject.mesh | Animals | - |
dc.subject.mesh | Bone Marrow Cells - cytology | - |
dc.subject.mesh | Cell Differentiation | - |
dc.subject.mesh | Cell Lineage | - |
dc.subject.mesh | Schwann Cells - cytology | - |
dc.title | Bone marrow-derived Schwann cells achieve fate commitment - a prerequisite for remyelination therapy | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-4886&volume=224&issue=2&spage=448&epage=458&date=2010&atitle=Bone+marrow-derived+Schwann+cells+achieve+fate+commitment+–+a+prerequisite+for+remyelination+therapy | en_HK |
dc.identifier.email | Shea, GKH: gkshea@hku.hk | en_HK |
dc.identifier.email | Chan, YS: yschan@hkucc.hku.hk | en_HK |
dc.identifier.email | Shum, DKY: shumdkhk@hkucc.hku.hk | en_HK |
dc.identifier.authority | Shea, GKH=rp01781 | en_HK |
dc.identifier.authority | Chan, YS=rp00318 | en_HK |
dc.identifier.authority | Shum, DKY=rp00321 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.expneurol.2010.05.005 | en_HK |
dc.identifier.pmid | 20483356 | - |
dc.identifier.scopus | eid_2-s2.0-77954658180 | en_HK |
dc.identifier.hkuros | 174275 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77954658180&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 224 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 448 | en_HK |
dc.identifier.epage | 458 | en_HK |
dc.identifier.eissn | 1090-2430 | - |
dc.identifier.isi | WOS:000280339600015 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Shea, GKH=36341273600 | en_HK |
dc.identifier.scopusauthorid | Tsui, AYP=36341669100 | en_HK |
dc.identifier.scopusauthorid | Chan, YS=7403676627 | en_HK |
dc.identifier.scopusauthorid | Shum, DKY=7004824447 | en_HK |
dc.identifier.citeulike | 7266695 | - |
dc.identifier.issnl | 0014-4886 | - |