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Article: Cumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass

TitleCumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass
Authors
Keywordsacrosome reaction
Cumulus oophorus
human spermatozoa
morphology
motility
Issue Date2009
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2009, v. 92 n. 3, p. 971-978 How to Cite?
AbstractObjective: To investigate the role of the cumulus cells and the cumulus matrix in affecting the penetrability, morphology, acrosome reaction, and motility of human spermatozoa penetrating the cumulus oophorus. Design: Controlled experimental laboratory study. Setting: University gynecology unit. Patient(s): Women undergoing assisted reproduction treatment and men visiting the subfertility clinics. Intervention(s): Human spermatozoa were allowed to penetrate through the cumulus oophorus and cell-depleted cumulus matrix in a capillary, and were treated with cumulus cell extract or hyaluronic acid. Main Outcome Measure(s): The morphology, acrosomal status, and motility of human spermatozoa were determined. Result(s): Fewer spermatozoa could penetrate the fresh cell-depleted matrix compared with intact cumulus oophorus. Spermatozoa that penetrated through the cumulus oophorus had higher percentages of normal morphology and acrosome reaction and had specific motility pattern. These effects were lost or reduced in the cell-depleted matrix that had been stored overnight. Hyaluronic acid, a main component of the cumulus matrix at concentration found in the cumulus oophorus, modulated sperm motility but did not affect spontaneous acrosome reaction. Cumulus cell extract did not affect sperm motility, but induced acrosome reaction. Conclusion(s): Both the cumulus matrix and the cumulus cells contribute to the effect of cumulus oophorus on spermatozoa penetrating through it. © 2009 American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/125307
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council, Hong KongHKU 7408/03M
Funding Information:

Supported by grants from the Research Grant Council, Hong Kong (HKU 7408/03M).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorHong, SJen_HK
dc.contributor.authorChiu, PCNen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorTse, JYMen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-10-31T11:23:37Z-
dc.date.available2010-10-31T11:23:37Z-
dc.date.issued2009en_HK
dc.identifier.citationFertility And Sterility, 2009, v. 92 n. 3, p. 971-978en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125307-
dc.description.abstractObjective: To investigate the role of the cumulus cells and the cumulus matrix in affecting the penetrability, morphology, acrosome reaction, and motility of human spermatozoa penetrating the cumulus oophorus. Design: Controlled experimental laboratory study. Setting: University gynecology unit. Patient(s): Women undergoing assisted reproduction treatment and men visiting the subfertility clinics. Intervention(s): Human spermatozoa were allowed to penetrate through the cumulus oophorus and cell-depleted cumulus matrix in a capillary, and were treated with cumulus cell extract or hyaluronic acid. Main Outcome Measure(s): The morphology, acrosomal status, and motility of human spermatozoa were determined. Result(s): Fewer spermatozoa could penetrate the fresh cell-depleted matrix compared with intact cumulus oophorus. Spermatozoa that penetrated through the cumulus oophorus had higher percentages of normal morphology and acrosome reaction and had specific motility pattern. These effects were lost or reduced in the cell-depleted matrix that had been stored overnight. Hyaluronic acid, a main component of the cumulus matrix at concentration found in the cumulus oophorus, modulated sperm motility but did not affect spontaneous acrosome reaction. Cumulus cell extract did not affect sperm motility, but induced acrosome reaction. Conclusion(s): Both the cumulus matrix and the cumulus cells contribute to the effect of cumulus oophorus on spermatozoa penetrating through it. © 2009 American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectacrosome reactionen_HK
dc.subjectCumulus oophorusen_HK
dc.subjecthuman spermatozoaen_HK
dc.subjectmorphologyen_HK
dc.subjectmotilityen_HK
dc.subject.meshAcrosome Reaction - drug effects - physiology-
dc.subject.meshCumulus Cells - physiology-
dc.subject.meshExtracellular Matrix - physiology-
dc.subject.meshSperm-Ovum Interactions - drug effects - physiology-
dc.subject.meshSpermatozoa - drug effects - physiology-
dc.titleCumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus massen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=92&issue=3&spage=971&epage=978&date=2009&atitle=Cumulus+cells+and+their+extracellular+matrix+affect+the+quality+of+the+spermatozoa+penetrating+the+cumulus+mass-
dc.identifier.emailChiu, PCN:pchiucn@hku.hken_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityChiu, PCN=rp00424en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.fertnstert.2008.07.1760en_HK
dc.identifier.pmid18930198en_HK
dc.identifier.scopuseid_2-s2.0-69249203767en_HK
dc.identifier.hkuros173272en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69249203767&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume92en_HK
dc.identifier.issue3en_HK
dc.identifier.spage971en_HK
dc.identifier.epage978en_HK
dc.identifier.isiWOS:000269711700026-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectRole of glycodelin in fertilization-
dc.identifier.scopusauthoridHong, SJ=7405767201en_HK
dc.identifier.scopusauthoridChiu, PCN=25959969200en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridTse, JYM=7102607314en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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