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Article: CD40-activated B cells are more potent than immature dendritic cells to induce and expand CD4 regulatory T cells

TitleCD40-activated B cells are more potent than immature dendritic cells to induce and expand CD4 regulatory T cells
Authors
KeywordsB cells
IL-2
Immature DC
Treg
Issue Date2010
PublisherChinese Society of Immunology. The Journal's web site is located at http://www.nature.com/cmi/index.html
Citation
Cellular And Molecular Immunology, 2010, v. 7 n. 1, p. 44-50 How to Cite?
AbstractCD4 + regulatory T cells (Tregs) play an important role in maintaining immune tolerance by suppressing pathologic immune responses. The generation of large numbers of antigen-specific Tregs ex vivo is critical for the development of clinical immunotherapy based on the adoptive transfer of Tregs. Both CD40-activated B cells (CD40-B) and immature dendritic cells (imDCs) have been used as professional antigen-presenting cells (APCs) to generate antigen-specific Tregs. However, the efficiencies of CD40-B and imDCs to generate CD4 + Tregs have not been compared directly and the mechanism driving the generation of these Tregs remains largely unknown. In this study, we found that CD40-B exhibited mature phenotypes and were more able to induce and expand CD4 highCD25 + Tregs than imDCs. Moreover, Tregs induced by CD40-B had greater suppressive capacity than those induced by imDCs. The generation of CD4 high CD25 + Tregs by CD40-B and imDCs is cell-cell contact dependent and partially relies on the expression of human leukocyte antigen (HLA)-DR and CD80/86. Differences in CD4 highCD25 +Treg generation efficiency were largely explained by the production of endogenous IL-2 by CD40-B. Our results suggest that CD40-B is better able to generate large numbers of antigen-specific Tregs than imDCs. Additionally, using CD40-B to generate Tregs may accelerate the clinical use of Treg-based immunotherapy in the treatment of allograft rejection, graft versus host disease (GVHD) and autoimmune diseases. © 2010 CSI and USTC. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/125226
ISSN
2015 Impact Factor: 5.193
2015 SCImago Journal Rankings: 1.898
ISI Accession Number ID
Funding AgencyGrant Number
University Research Committee
University of Hong Kong, Hong Kong, China
Research Grants Council of Hong Kong, Hong Kong, China
University Grants Committee, Hong Kong, ChinaoE/M-12/06
Edward Sai-Kim Hotung Paediatric Education
Funding Information:

This work was supported in part by the Seed Funding for Basic Research, University Research Committee, the University of Hong Kong (HKU), Hong Kong, China (WT); General Research fund, Research Grants Council of Hong Kong, Hong Kong, China (WT); the Area of Excellence Scheme of the University Grants Committee, Hong Kong, China (Grant AoE/M-12/06; WT and YLL); Edward Sai-Kim Hotung Paediatric Education and Research Fund (YLL, WT) and HKU postgraduate studentships (JZ).

References

 

DC FieldValueLanguage
dc.contributor.authorZheng, Jen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorLau, YLen_HK
dc.contributor.authorTu, Wen_HK
dc.date.accessioned2010-10-31T11:18:38Z-
dc.date.available2010-10-31T11:18:38Z-
dc.date.issued2010en_HK
dc.identifier.citationCellular And Molecular Immunology, 2010, v. 7 n. 1, p. 44-50en_HK
dc.identifier.issn1672-7681en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125226-
dc.description.abstractCD4 + regulatory T cells (Tregs) play an important role in maintaining immune tolerance by suppressing pathologic immune responses. The generation of large numbers of antigen-specific Tregs ex vivo is critical for the development of clinical immunotherapy based on the adoptive transfer of Tregs. Both CD40-activated B cells (CD40-B) and immature dendritic cells (imDCs) have been used as professional antigen-presenting cells (APCs) to generate antigen-specific Tregs. However, the efficiencies of CD40-B and imDCs to generate CD4 + Tregs have not been compared directly and the mechanism driving the generation of these Tregs remains largely unknown. In this study, we found that CD40-B exhibited mature phenotypes and were more able to induce and expand CD4 highCD25 + Tregs than imDCs. Moreover, Tregs induced by CD40-B had greater suppressive capacity than those induced by imDCs. The generation of CD4 high CD25 + Tregs by CD40-B and imDCs is cell-cell contact dependent and partially relies on the expression of human leukocyte antigen (HLA)-DR and CD80/86. Differences in CD4 highCD25 +Treg generation efficiency were largely explained by the production of endogenous IL-2 by CD40-B. Our results suggest that CD40-B is better able to generate large numbers of antigen-specific Tregs than imDCs. Additionally, using CD40-B to generate Tregs may accelerate the clinical use of Treg-based immunotherapy in the treatment of allograft rejection, graft versus host disease (GVHD) and autoimmune diseases. © 2010 CSI and USTC. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherChinese Society of Immunology. The Journal's web site is located at http://www.nature.com/cmi/index.htmlen_HK
dc.relation.ispartofCellular and Molecular Immunologyen_HK
dc.subjectB cellsen_HK
dc.subjectIL-2en_HK
dc.subjectImmature DCen_HK
dc.subjectTregen_HK
dc.titleCD40-activated B cells are more potent than immature dendritic cells to induce and expand CD4 regulatory T cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=2042-0226 (Electronic) 1672-7681 (Linkin&volume=7&issue=1&spage=44&epage=50&date=2010&atitle=CD40-activated+B+cells+are+more+potent+than+immature+dendritic+cells+to+induce+and+expand+CD4(+)+regulatory+T+cellsen_HK
dc.identifier.emailLiu, Y:yinpingl@hku.hken_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.emailTu, W:wwtu@hkucc.hku.hken_HK
dc.identifier.authorityLiu, Y=rp00269en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.identifier.authorityTu, W=rp00416en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/cmi.2009.103en_HK
dc.identifier.pmid20081875-
dc.identifier.scopuseid_2-s2.0-75949123497en_HK
dc.identifier.hkuros179385en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-75949123497&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue1en_HK
dc.identifier.spage44en_HK
dc.identifier.epage50en_HK
dc.identifier.eissn2042-0226-
dc.identifier.isiWOS:000274323900006-
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridZheng, J=55217878700en_HK
dc.identifier.scopusauthoridLiu, Y=35240639600en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.scopusauthoridTu, W=7006479236en_HK
dc.identifier.citeulike7014435-

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