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Article: Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs

TitleMinimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs
Authors
KeywordsAnnulus fibrosus
Cryopreservation
Dimethylsulphoxide
Intervertebral disc
Metabolic activity
Nucleus pulposus
Propylene glycol
Transplantation
Issue Date2010
PublisherSwiss Society for Biomaterials. The Journal's web site is located at http://www.ecmjournal.org
Citation
European Cells And Materials, 2010, v. 19, p. 273-283 How to Cite?
AbstractSevere intervertebral disc (IVD) degeneration often requires disc excision and spinal fusion, which leads to loss of spinal segment mobility. Implantation of an allograft disc or tissue engineered disc construct emerges as an alternative to artificial disc replacement for preserving the motion of the degenerated level. Establishment of a bank of cadaveric or engineered cryopreserved discs enables size matching, and facilitates clinical management. However, there is a lack of understanding of the behaviour of disc cells during cryopreservation, as well as how to maximize their survival, such that disc graft properties can be preserved. Here, we report on the effect of alterations in cooling rates, cryoprotective agents (CPAs), and duration of precryopreservation incubation in CPA on cellular activity in whole porcine lumbar discs. Our results indicated that cooling rates of -0.3°C/min and -0.5°C /min resulted in the least loss of metabolic activity in nucleus pulposus (NP) and annulus fibrosus (AF) respectively, while metabolic activity is best maintained by using a combination of 10% dimethylsulphoxide (DMSO) and 10% propylene-glycol (PG) as CPA. By the use of such parameters, metabolic activity of the NP and the AF cells could be maintained at 70% and 45%, respectively, of that of the fresh tissue. Mechanical testing and histological evaluation showed no significant differences in mechanical properties or alterations in disc structure compared to fresh discs. Despite the limitations of the animal model, our findings provide a framework for establishing an applicable cryopreservation protocol for human disc allografts or tissue-engineered disc constructs.
Persistent Identifierhttp://hdl.handle.net/10722/125201
ISSN
2015 Impact Factor: 3.654
2015 SCImago Journal Rankings: 1.771
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, SCWen_HK
dc.contributor.authorLam, SKLen_HK
dc.contributor.authorLeung, VYLen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorCheung, KMCen_HK
dc.date.accessioned2010-10-31T11:17:11Z-
dc.date.available2010-10-31T11:17:11Z-
dc.date.issued2010en_HK
dc.identifier.citationEuropean Cells And Materials, 2010, v. 19, p. 273-283en_HK
dc.identifier.issn1473-2262en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125201-
dc.description.abstractSevere intervertebral disc (IVD) degeneration often requires disc excision and spinal fusion, which leads to loss of spinal segment mobility. Implantation of an allograft disc or tissue engineered disc construct emerges as an alternative to artificial disc replacement for preserving the motion of the degenerated level. Establishment of a bank of cadaveric or engineered cryopreserved discs enables size matching, and facilitates clinical management. However, there is a lack of understanding of the behaviour of disc cells during cryopreservation, as well as how to maximize their survival, such that disc graft properties can be preserved. Here, we report on the effect of alterations in cooling rates, cryoprotective agents (CPAs), and duration of precryopreservation incubation in CPA on cellular activity in whole porcine lumbar discs. Our results indicated that cooling rates of -0.3°C/min and -0.5°C /min resulted in the least loss of metabolic activity in nucleus pulposus (NP) and annulus fibrosus (AF) respectively, while metabolic activity is best maintained by using a combination of 10% dimethylsulphoxide (DMSO) and 10% propylene-glycol (PG) as CPA. By the use of such parameters, metabolic activity of the NP and the AF cells could be maintained at 70% and 45%, respectively, of that of the fresh tissue. Mechanical testing and histological evaluation showed no significant differences in mechanical properties or alterations in disc structure compared to fresh discs. Despite the limitations of the animal model, our findings provide a framework for establishing an applicable cryopreservation protocol for human disc allografts or tissue-engineered disc constructs.en_HK
dc.languageengen_HK
dc.publisherSwiss Society for Biomaterials. The Journal's web site is located at http://www.ecmjournal.orgen_HK
dc.relation.ispartofEuropean Cells and Materialsen_HK
dc.subjectAnnulus fibrosusen_HK
dc.subjectCryopreservationen_HK
dc.subjectDimethylsulphoxideen_HK
dc.subjectIntervertebral discen_HK
dc.subjectMetabolic activityen_HK
dc.subjectNucleus pulposusen_HK
dc.subjectPropylene glycolen_HK
dc.subjectTransplantationen_HK
dc.subject.meshAnimals-
dc.subject.meshCell Survival-
dc.subject.meshCold Temperature-
dc.subject.meshCryopreservation - methods - standards-
dc.subject.meshIntervertebral Disk - cytology - transplantation-
dc.titleMinimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1473-2262&volume=19&spage=273&epage=283&date=2010&atitle=Minimizing+cryopreservation-induced+loss+of+disc+cell+activity+for+storage+of+whole+intervertebral+discs-
dc.identifier.emailLeung, VYL: vicleung@hku.hken_HK
dc.identifier.emailChan, D: chand@hkucc.hku.hken_HK
dc.identifier.emailLuk, KDK: hcm21000@hku.hken_HK
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_HK
dc.identifier.authorityLeung, VYL=rp01764en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid20533193-
dc.identifier.scopuseid_2-s2.0-77955483525en_HK
dc.identifier.hkuros178268en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955483525&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.spage273en_HK
dc.identifier.epage283en_HK
dc.identifier.isiWOS:000278752500023-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridChan, SCW=55209636300en_HK
dc.identifier.scopusauthoridLam, SKL=36622142100en_HK
dc.identifier.scopusauthoridLeung, VYL=35337438900en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK

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