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Article: Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells

TitleMesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells
Authors
Issue Date2009
PublisherNature Publishing Group.
Citation
Molecular Therapy, 2009, v. 17 n. 11, p. 1959-1966 How to Cite?
AbstractDegenerative disc disease (DDD) is a common disease which affects millions of people. Autograft of the bone marrow derived mesenchymal stem cells (BMSCs) have been shown to have the ability to arrest degeneration in rabbit and canine intervertebral discs. In this study, we have used the mouse model to investigate the mechanism of degeneration arrest. BMSC from Egfp transgenic mice were injected into the degenerated murine intervertebral discs induced by annular puncture. We found that BMSC could arrest the progressive degeneration of the discs with significant regeneration of the nucleus pulposus (NP). In the regeneration, expression of proteoglycan genes were upregulated and extracellular matrix (ECM) progressively accumulated in the NP after BMSC injection. Combined in situ hybridization and immunohistochemistry revealed that BMSC underwent chondrocytic differentiation in the regeneration process. Interestingly, BMSC-induced an increase of endogenous notochordal cells in NP and expression of chondrocytic markers. In this study, we have firstly shown that the BMSC could arrest the degeneration of the murine notochordal NP and contribute to the augmentation of the ECM in the NP by both autonomous differentiation and stimulatory action on endogenous cells.
Persistent Identifierhttp://hdl.handle.net/10722/125194
ISSN
2015 Impact Factor: 6.938
2015 SCImago Journal Rankings: 3.374
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
CRCG
Funding Information:

This work is supported by the CRCG grant from the University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorYang, Fen_HK
dc.contributor.authorLeung, VYLen_HK
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorCheung, KMCen_HK
dc.date.accessioned2010-10-31T11:16:47Z-
dc.date.available2010-10-31T11:16:47Z-
dc.date.issued2009en_HK
dc.identifier.citationMolecular Therapy, 2009, v. 17 n. 11, p. 1959-1966en_HK
dc.identifier.issn1525-0016en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125194-
dc.description.abstractDegenerative disc disease (DDD) is a common disease which affects millions of people. Autograft of the bone marrow derived mesenchymal stem cells (BMSCs) have been shown to have the ability to arrest degeneration in rabbit and canine intervertebral discs. In this study, we have used the mouse model to investigate the mechanism of degeneration arrest. BMSC from Egfp transgenic mice were injected into the degenerated murine intervertebral discs induced by annular puncture. We found that BMSC could arrest the progressive degeneration of the discs with significant regeneration of the nucleus pulposus (NP). In the regeneration, expression of proteoglycan genes were upregulated and extracellular matrix (ECM) progressively accumulated in the NP after BMSC injection. Combined in situ hybridization and immunohistochemistry revealed that BMSC underwent chondrocytic differentiation in the regeneration process. Interestingly, BMSC-induced an increase of endogenous notochordal cells in NP and expression of chondrocytic markers. In this study, we have firstly shown that the BMSC could arrest the degeneration of the murine notochordal NP and contribute to the augmentation of the ECM in the NP by both autonomous differentiation and stimulatory action on endogenous cells.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group.-
dc.relation.ispartofMolecular Therapyen_HK
dc.subject.meshCell Differentiation - physiology-
dc.subject.meshChondrocytes - cytology - metabolism-
dc.subject.meshIntervertebral Disk Degeneration - therapy-
dc.subject.meshMesenchymal Stem Cell Transplantation - methods-
dc.subject.meshTissue Therapy - methods-
dc.titleMesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1525-0016&volume=17&issue=11&spage=1959&epage=1966&date=2009&atitle=Mesenchymal+stem+cells+arrest+intervertebral+disc+degeneration+through+chondrocytic+differentiation+and+stimulation+of+endogenous+cellsen_HK
dc.identifier.emailLeung, VYL: vicleung@hku.hken_HK
dc.identifier.emailLuk, KDK: hcm21000@hku.hken_HK
dc.identifier.emailChan, D: chand@hkucc.hku.hken_HK
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_HK
dc.identifier.authorityLeung, VYL=rp01764en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1038/mt.2009.146en_HK
dc.identifier.pmid19584814-
dc.identifier.pmcidPMC2835041-
dc.identifier.scopuseid_2-s2.0-70449133790en_HK
dc.identifier.hkuros180187en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70449133790&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1959en_HK
dc.identifier.epage1966en_HK
dc.identifier.eissn1525-0024-
dc.identifier.isiWOS:000271835000017-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYang, F=35354574000en_HK
dc.identifier.scopusauthoridLeung, VYL=35337438900en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK

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