Article: Biochemical characterization of the cell-biomaterial interface by quantitative proteomics

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TitleBiochemical characterization of the cell-biomaterial interface by quantitative proteomics
AuthorsTong, WY2
Liang, YM3
Tam, V2
Yip, HK4
Kao, YT1
Cheung, KMC2
Yeung, KWK2
Lam, YW3
Issue Date2010
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc.. The Journal's web site is located at http://www.mcponline.org/
CitationMolecular And Cellular Proteomics, 2010, v. 9 n. 10, p. 2089-2098 [How to Cite?]
DOI: http://dx.doi.org/10.1074/mcp.M110.001966
AbstractSurface topography and texture of cell culture substrata can affect the differentiation and growth of adherent cells. The biochemical basis of the transduction of the physical and mechanical signals to cellular responses is not well understood. The lack of a systematic characterization of cell-biomaterial interaction is the major bottleneck. This study demonstrated the use of a novel subcellular fractionation method combined with quantitative MS-based proteomics to enable the robust and high-throughput analysis of proteins at the adherence interface of Madin-Darby canine kidney cells. This method revealed the enrichment of extracellular matrix proteins and membrane and stress fibers proteins at the adherence surface, whereas it shows depletion of extracellular matrix belonging to the cytoplasmic, nucleus, and lateral and apical membranes. The asymmetric distribution of proteins between apical and adherence sides was also profiled. Apart from classical proteins with clear involvement in cell-material interactions, proteins previously not known to be involved in cell attachment were also discovered. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
ISSN1535-9476
2011 Impact Factor: 7.398
2011 SCImago Journal Rankings: 1.273
DOIhttp://dx.doi.org/10.1074/mcp.M110.001966
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorTong, WY
dc.contributor.authorLiang, YM
dc.contributor.authorTam, V
dc.contributor.authorYip, HK
dc.contributor.authorKao, YT
dc.contributor.authorCheung, KMC
dc.contributor.authorYeung, KWK
dc.contributor.authorLam, YW
dc.date.accessioned2010-10-31T11:16:28Z
dc.date.available2010-10-31T11:16:28Z
dc.date.issued2010
dc.description.abstractSurface topography and texture of cell culture substrata can affect the differentiation and growth of adherent cells. The biochemical basis of the transduction of the physical and mechanical signals to cellular responses is not well understood. The lack of a systematic characterization of cell-biomaterial interaction is the major bottleneck. This study demonstrated the use of a novel subcellular fractionation method combined with quantitative MS-based proteomics to enable the robust and high-throughput analysis of proteins at the adherence interface of Madin-Darby canine kidney cells. This method revealed the enrichment of extracellular matrix proteins and membrane and stress fibers proteins at the adherence surface, whereas it shows depletion of extracellular matrix belonging to the cytoplasmic, nucleus, and lateral and apical membranes. The asymmetric distribution of proteins between apical and adherence sides was also profiled. Apart from classical proteins with clear involvement in cell-material interactions, proteins previously not known to be involved in cell attachment were also discovered. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationMolecular And Cellular Proteomics, 2010, v. 9 n. 10, p. 2089-2098 [How to Cite?]
DOI: http://dx.doi.org/10.1074/mcp.M110.001966
dc.identifier.doihttp://dx.doi.org/10.1074/mcp.M110.001966
dc.identifier.eissn1535-9484
dc.identifier.epage2098
dc.identifier.hkuros178904
dc.identifier.isiWOS:000282368900001
dc.identifier.issn1535-9476
2011 Impact Factor: 7.398
2011 SCImago Journal Rankings: 1.273
dc.identifier.issue10
dc.identifier.openurl
dc.identifier.pmid20562470
dc.identifier.scopuseid_2-s2.0-77957977633
dc.identifier.spage2089
dc.identifier.urihttp://hdl.handle.net/10722/125188
dc.identifier.volume9
dc.languageeng
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc.. The Journal's web site is located at http://www.mcponline.org/
dc.publisher.placeUnited States
dc.relation.ispartofMolecular and Cellular Proteomics
dc.relation.referencesReferences in Scopus
dc.titleBiochemical characterization of the cell-biomaterial interface by quantitative proteomics
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
  3. City University of Hong Kong
  4. James Cook University