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Article: Nanostructure of collagen fibrils in human nucleus pulposus and its correlation with macroscale tissue mechanics

TitleNanostructure of collagen fibrils in human nucleus pulposus and its correlation with macroscale tissue mechanics
Authors
KeywordsAtomic force microscope
Collagen fibril
Human intervertebral disc
Nanostructure
Nucleus pulposus
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthres
Citation
Journal Of Orthopaedic Research, 2010, v. 28 n. 4, p. 497-502 How to Cite?
AbstractCollagen fibrils are the main structural components of the nucleus pulposus tissue in the intervertebral discs. The structure-property relationship of the nucleus pulposus (NP) tissues is still unclear. We investigated the structure of individual collagen fibrils of the NP and evaluated its correlation with the bulk mechanical properties of the tissue. Collagen fibrils were extracted from the NP of discs retrieved from adolescents during scoliosis correction surgery, and the extracts were confirmed by SDS-PAGE. The diameters of the individual collagen fibrils were measured through atomic force microscopy, and the compressive mechanical properties of the tissues were evaluated by confined compression. The correlations between the nanoscale morphology of the collagen fibrils and the macroscale mechanical properties of the tissues were evaluated by linear regression. The SDS-PAGE results showed that the fibril extracts were largely composed of type II collagen. The mean diameter of the collagen fibrils was 92.1 ± 26.54 nm; the mean swelling pressure and compressive modulus of the tissues were 6.15 ± 4.3 kPa and 1.23 ± 0.7 MPa, respectively. The mean fibril diameter had no linear correlation (R2 = 0.30) with the swelling pressure of the tissues. However, it had a mild linear correlation with the compressive modulus (p = 0.023, R2 = 0.68). This is the first study, to our knowledge, to evaluate the nanostructure of the individual collagen fibrils of the nucleus pulposus and its relationship with macroscale mechanical properties of the NP tissues. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/125179
ISSN
2015 Impact Factor: 2.807
2015 SCImago Journal Rankings: 1.464
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7378/04M
HKU 7153/06E
HKU 7149/08E
University Grants CommitteeAoE/M-04/04
University Research Committee of the University of Hong Kong10206180
Global Enterprise for Micro Mechanics and Molecular Medicine (GEM4) Lab at the National University of Singapore
Funding Information:

This work was supported by grants from the Hong Kong Research Grants Council (HKU 7378/04M, HKU 7153/06E HKU 7149/08E), University Grants Committee (AoE/M-04/04), and University Research Committee of the University of Hong Kong (10206180). We thank Patrick Y. P. Kao, Dr. Kwok Y. Tsang, and Miss. Rebecca L. K. Lo for their technical assistance. Chwee Teck Lim gratefully acknowledges support provided by the Global Enterprise for Micro Mechanics and Molecular Medicine (GEM4) Lab at the National University of Singapore.

References
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DC FieldValueLanguage
dc.contributor.authorAladin, DMKen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorNgan, AHWen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorLeung, VYLen_HK
dc.contributor.authorLim, CTen_HK
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorLu, WWen_HK
dc.date.accessioned2010-10-31T11:15:57Z-
dc.date.available2010-10-31T11:15:57Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Orthopaedic Research, 2010, v. 28 n. 4, p. 497-502en_HK
dc.identifier.issn0736-0266en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125179-
dc.description.abstractCollagen fibrils are the main structural components of the nucleus pulposus tissue in the intervertebral discs. The structure-property relationship of the nucleus pulposus (NP) tissues is still unclear. We investigated the structure of individual collagen fibrils of the NP and evaluated its correlation with the bulk mechanical properties of the tissue. Collagen fibrils were extracted from the NP of discs retrieved from adolescents during scoliosis correction surgery, and the extracts were confirmed by SDS-PAGE. The diameters of the individual collagen fibrils were measured through atomic force microscopy, and the compressive mechanical properties of the tissues were evaluated by confined compression. The correlations between the nanoscale morphology of the collagen fibrils and the macroscale mechanical properties of the tissues were evaluated by linear regression. The SDS-PAGE results showed that the fibril extracts were largely composed of type II collagen. The mean diameter of the collagen fibrils was 92.1 ± 26.54 nm; the mean swelling pressure and compressive modulus of the tissues were 6.15 ± 4.3 kPa and 1.23 ± 0.7 MPa, respectively. The mean fibril diameter had no linear correlation (R2 = 0.30) with the swelling pressure of the tissues. However, it had a mild linear correlation with the compressive modulus (p = 0.023, R2 = 0.68). This is the first study, to our knowledge, to evaluate the nanostructure of the individual collagen fibrils of the nucleus pulposus and its relationship with macroscale mechanical properties of the NP tissues. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthresen_HK
dc.relation.ispartofJournal of Orthopaedic Researchen_HK
dc.rightsJournal of Orthopaedic Research. Copyright © John Wiley & Sons, Inc.-
dc.subjectAtomic force microscopeen_HK
dc.subjectCollagen fibrilen_HK
dc.subjectHuman intervertebral discen_HK
dc.subjectNanostructureen_HK
dc.subjectNucleus pulposusen_HK
dc.subject.meshAdolescent-
dc.subject.meshBiomechanics-
dc.subject.meshFibrillar Collagens - chemistry - ultrastructure-
dc.subject.meshIntervertebral Disk - chemistry-
dc.subject.meshNanofibers - chemistry - ultrastructure-
dc.titleNanostructure of collagen fibrils in human nucleus pulposus and its correlation with macroscale tissue mechanicsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0736-0266&volume=28&issue=4&spage=497&epage=502&date=2010&atitle=Nano-structure+of+collagen+fibrils+in+human+nucleus+pulposus+and+its+correlation+with+macroscale+tissue+mechanics-
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_HK
dc.identifier.emailNgan, AHW: hwngan@hkucc.hku.hken_HK
dc.identifier.emailChan, D: chand@hkucc.hku.hken_HK
dc.identifier.emailLeung, VYL: vicleung@hku.hken_HK
dc.identifier.emailLuk, KDK: hcm21000@hku.hken_HK
dc.identifier.emailLu, WW: wwlu@hku.hken_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityNgan, AHW=rp00225en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityLeung, VYL=rp01764en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jor.21010en_HK
dc.identifier.pmid19862800en_HK
dc.identifier.scopuseid_2-s2.0-77949505319en_HK
dc.identifier.hkuros178111en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77949505319&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue4en_HK
dc.identifier.spage497en_HK
dc.identifier.epage502en_HK
dc.identifier.isiWOS:000275958900013-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectThe micro-structure and micro-mechanics of human intervertebral disc-
dc.relation.projectThe impact of the COL9A2 Q326W allele on intervertebral disc structure and degeneration-
dc.relation.projectDevelopmental genomics and skeletal research-
dc.identifier.scopusauthoridAladin, DMK=23491673700en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridNgan, AHW=7006827202en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridLeung, VYL=35337438900en_HK
dc.identifier.scopusauthoridLim, CT=7403654651en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.scopusauthoridLu, WW=55484358100en_HK

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