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Article: A 219-mer CHO-expressing receptor-binding domain of SARS-CoV S protein induces potent immune responses and protective immunity

TitleA 219-mer CHO-expressing receptor-binding domain of SARS-CoV S protein induces potent immune responses and protective immunity
Authors
Issue Date2010
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/vim
Citation
Viral Immunology, 2010, v. 23 n. 2, p. 211-219 How to Cite?
AbstractDevelopment of vaccines is essential for the prevention of future recurrences of severe acute respiratory syndrome (SARS), caused by the SARS coronavirus (SARS-CoV). The spike (S) protein, especially receptor-binding domain (RBD) of SARS-CoV, plays important roles in the prevention of SARS infection, and is thus an important component in SARS vaccine development. In this study, we expressed a 219-mer (residues 318-536) RBD protein in Chinese hamster ovary (CHO)-K1 cells (RBD219-CHO), and tested its immune responses and protective immunity in a mouse model. The results showed that this recombinant protein was correctly folded, being able to maintain intact conformation and authentic antigenicity. It could induce strong humoral and cellular immune responses and high titers of neutralizing antibodies in the vaccinated mice. RBD219-CHO protein elicited potent protective immunity that protected all vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD219-CHO protein has great potential for the development of an effective and safe SARS subunit vaccine. © Copyright 2010, Mary Ann Liebert, Inc..
Persistent Identifierhttp://hdl.handle.net/10722/125154
ISSN
2014 Impact Factor: 1.446
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of Health (NIH)RO1 AI68002
Hong Kong SAR Government
National 973 Basic Research Program of China2005CB523001
Funding Information:

This study was supported by the National Institutes of Health (NIH) of the United States (RO1 AI68002), by the Research Fund for the Control of Infectious Diseases, the Health, Welfare and Food Bureau of the Hong Kong SAR Government, and by the National 973 Basic Research Program of China (2005CB523001).

References

 

DC FieldValueLanguage
dc.contributor.authorDu, Len_HK
dc.contributor.authorZhao, Gen_HK
dc.contributor.authorChan, CCen_HK
dc.contributor.authorLi, Len_HK
dc.contributor.authorHe, Yen_HK
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorJiang, Sen_HK
dc.date.accessioned2010-10-31T11:14:27Z-
dc.date.available2010-10-31T11:14:27Z-
dc.date.issued2010en_HK
dc.identifier.citationViral Immunology, 2010, v. 23 n. 2, p. 211-219en_HK
dc.identifier.issn0882-8245en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125154-
dc.description.abstractDevelopment of vaccines is essential for the prevention of future recurrences of severe acute respiratory syndrome (SARS), caused by the SARS coronavirus (SARS-CoV). The spike (S) protein, especially receptor-binding domain (RBD) of SARS-CoV, plays important roles in the prevention of SARS infection, and is thus an important component in SARS vaccine development. In this study, we expressed a 219-mer (residues 318-536) RBD protein in Chinese hamster ovary (CHO)-K1 cells (RBD219-CHO), and tested its immune responses and protective immunity in a mouse model. The results showed that this recombinant protein was correctly folded, being able to maintain intact conformation and authentic antigenicity. It could induce strong humoral and cellular immune responses and high titers of neutralizing antibodies in the vaccinated mice. RBD219-CHO protein elicited potent protective immunity that protected all vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD219-CHO protein has great potential for the development of an effective and safe SARS subunit vaccine. © Copyright 2010, Mary Ann Liebert, Inc..en_HK
dc.languageengen_HK
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/vimen_HK
dc.relation.ispartofViral Immunologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a copy of an article published in the [Viral Immunology] © [2010] [copyright Mary Ann Liebert, Inc.]; [Viral Immunology} is available online at: http://www.liebertonline.com.-
dc.subject.meshMembrane Glycoproteins - chemistry - immunology-
dc.subject.meshReceptors, Virus - metabolism-
dc.subject.meshSARS Virus - immunology-
dc.subject.meshSevere Acute Respiratory Syndrome - immunology - prevention and control-
dc.subject.meshViral Envelope Proteins - chemistry - immunology-
dc.titleA 219-mer CHO-expressing receptor-binding domain of SARS-CoV S protein induces potent immune responses and protective immunityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0882-8245&volume=23&issue=2&spage=211&epage=219&date=2010&atitle=A+219-mer+CHO-expressing+RBD+of+SARS-CoV+S+protein+induces+potent+immune+responses+and+protective+immunity-
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1089/vim.2009.0090en_HK
dc.identifier.pmid20374001-
dc.identifier.pmcidPMC2883479-
dc.identifier.scopuseid_2-s2.0-77953940733en_HK
dc.identifier.hkuros175102en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77953940733&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue2en_HK
dc.identifier.spage211en_HK
dc.identifier.epage219en_HK
dc.identifier.isiWOS:000276413200010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDu, L=8686996200en_HK
dc.identifier.scopusauthoridZhao, G=8684553000en_HK
dc.identifier.scopusauthoridChan, CC=16021156900en_HK
dc.identifier.scopusauthoridLi, L=36072593200en_HK
dc.identifier.scopusauthoridHe, Y=8742157400en_HK
dc.identifier.scopusauthoridZhou, Y=8791655300en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridJiang, S=7404453146en_HK

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