Article: A 219-mer CHO-expressing receptor-binding domain of SARS-CoV S protein induces potent immune responses and protective immunity
File Download
Links for fulltext
(May Require Subscription)
(May Require Subscription)
- Publisher Website: 10.1089/vim.2009.0090
- Scopus: eid_2-s2.0-77953940733
- PMID: 20374001
- Find via
Supplementary
-
Citations:
-
Appears in Collections:
| Title | A 219-mer CHO-expressing receptor-binding domain of SARS-CoV S protein induces potent immune responses and protective immunity | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Authors | Du, L3 Zhao, G1 2 Chan, CC2 Li, L3 He, Y3 Zhou, Y1 Zheng, BJ2 Jiang, S3 | ||||||||
| Issue Date | 2010 | ||||||||
| Publisher | Mary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/vim | ||||||||
| Citation | Viral Immunology, 2010, v. 23 n. 2, p. 211-219 [How to Cite?] DOI: http://dx.doi.org/10.1089/vim.2009.0090 | ||||||||
| Abstract | Development of vaccines is essential for the prevention of future recurrences of severe acute respiratory syndrome (SARS), caused by the SARS coronavirus (SARS-CoV). The spike (S) protein, especially receptor-binding domain (RBD) of SARS-CoV, plays important roles in the prevention of SARS infection, and is thus an important component in SARS vaccine development. In this study, we expressed a 219-mer (residues 318-536) RBD protein in Chinese hamster ovary (CHO)-K1 cells (RBD219-CHO), and tested its immune responses and protective immunity in a mouse model. The results showed that this recombinant protein was correctly folded, being able to maintain intact conformation and authentic antigenicity. It could induce strong humoral and cellular immune responses and high titers of neutralizing antibodies in the vaccinated mice. RBD219-CHO protein elicited potent protective immunity that protected all vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD219-CHO protein has great potential for the development of an effective and safe SARS subunit vaccine. © Copyright 2010, Mary Ann Liebert, Inc.. | ||||||||
| ISSN | 0882-8245 2011 Impact Factor: 1.966 2011 SCImago Journal Rankings: 0.223 | ||||||||
| DOI | http://dx.doi.org/10.1089/vim.2009.0090 | ||||||||
| ISI Accession Number ID | WOS:000276413200010
Funding Information: This study was supported by the National Institutes of Health (NIH) of the United States (RO1 AI68002), by the Research Fund for the Control of Infectious Diseases, the Health, Welfare and Food Bureau of the Hong Kong SAR Government, and by the National 973 Basic Research Program of China (2005CB523001). | ||||||||
| PubMed Central ID | PMC2883479 | ||||||||
| References | References in Scopus |
| dc.contributor.author | Du, L | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Zhao, G | ||||||||
| dc.contributor.author | Chan, CC | ||||||||
| dc.contributor.author | Li, L | ||||||||
| dc.contributor.author | He, Y | ||||||||
| dc.contributor.author | Zhou, Y | ||||||||
| dc.contributor.author | Zheng, BJ | ||||||||
| dc.contributor.author | Jiang, S | ||||||||
| dc.date.accessioned | 2010-10-31T11:14:27Z | ||||||||
| dc.date.available | 2010-10-31T11:14:27Z | ||||||||
| dc.date.issued | 2010 | ||||||||
| dc.description.abstract | Development of vaccines is essential for the prevention of future recurrences of severe acute respiratory syndrome (SARS), caused by the SARS coronavirus (SARS-CoV). The spike (S) protein, especially receptor-binding domain (RBD) of SARS-CoV, plays important roles in the prevention of SARS infection, and is thus an important component in SARS vaccine development. In this study, we expressed a 219-mer (residues 318-536) RBD protein in Chinese hamster ovary (CHO)-K1 cells (RBD219-CHO), and tested its immune responses and protective immunity in a mouse model. The results showed that this recombinant protein was correctly folded, being able to maintain intact conformation and authentic antigenicity. It could induce strong humoral and cellular immune responses and high titers of neutralizing antibodies in the vaccinated mice. RBD219-CHO protein elicited potent protective immunity that protected all vaccinated mice from SARS-CoV challenge. These results suggest that the recombinant RBD219-CHO protein has great potential for the development of an effective and safe SARS subunit vaccine. © Copyright 2010, Mary Ann Liebert, Inc.. | ||||||||
| dc.description.nature | published_or_final_version | ||||||||
| dc.identifier.citation | Viral Immunology, 2010, v. 23 n. 2, p. 211-219 [How to Cite?] DOI: http://dx.doi.org/10.1089/vim.2009.0090 | ||||||||
| dc.identifier.doi | http://dx.doi.org/10.1089/vim.2009.0090 | ||||||||
| dc.identifier.epage | 219 | ||||||||
| dc.identifier.hkuros | 175102 | ||||||||
| dc.identifier.isi | WOS:000276413200010
Funding Information: This study was supported by the National Institutes of Health (NIH) of the United States (RO1 AI68002), by the Research Fund for the Control of Infectious Diseases, the Health, Welfare and Food Bureau of the Hong Kong SAR Government, and by the National 973 Basic Research Program of China (2005CB523001). | ||||||||
| dc.identifier.issn | 0882-8245 2011 Impact Factor: 1.966 2011 SCImago Journal Rankings: 0.223 | ||||||||
| dc.identifier.issue | 2 | ||||||||
| dc.identifier.openurl | | ||||||||
| dc.identifier.pmcid | PMC2883479 | ||||||||
| dc.identifier.pmid | 20374001 | ||||||||
| dc.identifier.scopus | eid_2-s2.0-77953940733 | ||||||||
| dc.identifier.spage | 211 | ||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/125154 | ||||||||
| dc.identifier.volume | 23 | ||||||||
| dc.language | eng | ||||||||
| dc.publisher | Mary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/vim | ||||||||
| dc.publisher.place | United States | ||||||||
| dc.relation.ispartof | Viral Immunology | ||||||||
| dc.relation.references | References in Scopus | ||||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||||
| dc.rights | This is a copy of an article published in the [JOURNAL TITLE] © [year of publication] [copyright Mary Ann Liebert, Inc.]; [JOURNAL TITLE} is available online at: http://www.liebertonline.com. | ||||||||
| dc.subject.mesh | Membrane Glycoproteins - chemistry - immunology | ||||||||
| dc.subject.mesh | Receptors, Virus - metabolism | ||||||||
| dc.subject.mesh | SARS Virus - immunology | ||||||||
| dc.subject.mesh | Severe Acute Respiratory Syndrome - immunology - prevention and control | ||||||||
| dc.subject.mesh | Viral Envelope Proteins - chemistry - immunology | ||||||||
| dc.title | A 219-mer CHO-expressing receptor-binding domain of SARS-CoV S protein induces potent immune responses and protective immunity | ||||||||
| dc.type | Article |
Author Affiliations
- Institute of Microbiology Chinese Academy of Sciences
- The University of Hong Kong
- New York Blood Center