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Article: Challenge and polymorphism analysis of the novel A (H1N1) influenza virus to normal animals

TitleChallenge and polymorphism analysis of the novel A (H1N1) influenza virus to normal animals
Authors
KeywordsA (H1N1) influenza
Animal challenge
Genome alignment
Propagation of virus
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusres
Citation
Virus Research, 2010, v. 151 n. 1, p. 60-65 How to Cite?
AbstractThe novel influenza A (H1N1) virus that emerged from April 2009 in Mexico has spread rapidly to many countries and initiated a human pandemic. It is important to determine whether the virus has existed in, or will spread to, normal household animals, and whether A (H1N1)-like viruses derived from the animal is able to proliferate in cell lines derived from human. In this current paper, familiar animals, including pigs, chickens, ducks, cats, dogs, rats, mice, and Brandt's voles were challenged with the novel influenza A (H1N1) virus, and genetic variations of the viral genome were analyzed after three passages in the susceptible animals. To further determine the virulence of these animals derived influenza A (H1N1)-like viruses, viral replication dynamic curves were monitored after inoculation in MDCK cells and human A549 cells. Our results indicated that pigs, BALB/c mice, and Brandt's voles, but not chickens, ducks, cats, dogs, and rats, could be infected by the novel influenza A (H1N1) virus. Genome sequence alignment results showed that there was one genetic variation (G408T) in the HA gene of Brandt's vole derived virus and another one (C194A) in the NA gene of BALB/c mice derived virus, and the virulence of these two viruses in MDCK and A549 cells was significantly lower than the virus originally derived from human beings. © 2010 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/125152
ISSN
2015 Impact Factor: 2.526
2015 SCImago Journal Rankings: 1.259
ISI Accession Number ID
Funding AgencyGrant Number
Industry Foundation of Ministry of Health200802036
National Science and Technology Major Projects of Infectious Disease2009ZX10004-402
2009ZX10004-016
Funding Information:

The present work was supported by the Industry Foundation of Ministry of Health (200802036) and the National Science and Technology Major Projects of Infectious Disease (2009ZX10004-402 and 2009ZX10004-016).

References

 

DC FieldValueLanguage
dc.contributor.authorBao, Len_HK
dc.contributor.authorXu, Len_HK
dc.contributor.authorZhan, Len_HK
dc.contributor.authorDeng, Wen_HK
dc.contributor.authorZhu, Hen_HK
dc.contributor.authorGao, Hen_HK
dc.contributor.authorSun, Hen_HK
dc.contributor.authorMa, Cen_HK
dc.contributor.authorLv, Qen_HK
dc.contributor.authorLi, Fen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorZhang, Len_HK
dc.contributor.authorQin, Cen_HK
dc.date.accessioned2010-10-31T11:14:21Z-
dc.date.available2010-10-31T11:14:21Z-
dc.date.issued2010en_HK
dc.identifier.citationVirus Research, 2010, v. 151 n. 1, p. 60-65en_HK
dc.identifier.issn0168-1702en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125152-
dc.description.abstractThe novel influenza A (H1N1) virus that emerged from April 2009 in Mexico has spread rapidly to many countries and initiated a human pandemic. It is important to determine whether the virus has existed in, or will spread to, normal household animals, and whether A (H1N1)-like viruses derived from the animal is able to proliferate in cell lines derived from human. In this current paper, familiar animals, including pigs, chickens, ducks, cats, dogs, rats, mice, and Brandt's voles were challenged with the novel influenza A (H1N1) virus, and genetic variations of the viral genome were analyzed after three passages in the susceptible animals. To further determine the virulence of these animals derived influenza A (H1N1)-like viruses, viral replication dynamic curves were monitored after inoculation in MDCK cells and human A549 cells. Our results indicated that pigs, BALB/c mice, and Brandt's voles, but not chickens, ducks, cats, dogs, and rats, could be infected by the novel influenza A (H1N1) virus. Genome sequence alignment results showed that there was one genetic variation (G408T) in the HA gene of Brandt's vole derived virus and another one (C194A) in the NA gene of BALB/c mice derived virus, and the virulence of these two viruses in MDCK and A549 cells was significantly lower than the virus originally derived from human beings. © 2010 Elsevier B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusresen_HK
dc.relation.ispartofVirus Researchen_HK
dc.subjectA (H1N1) influenzaen_HK
dc.subjectAnimal challengeen_HK
dc.subjectGenome alignmenten_HK
dc.subjectPropagation of virusen_HK
dc.subject.meshAnimals-
dc.subject.meshInfluenza A Virus, H1N1 Subtype - genetics - pathogenicity-
dc.subject.meshInfluenza, Human - transmission-
dc.subject.meshOrthomyxoviridae Infections - pathology - transmission - veterinary-
dc.subject.meshPolymorphism, Genetic-
dc.titleChallenge and polymorphism analysis of the novel A (H1N1) influenza virus to normal animalsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-1702&volume=151&issue=1&spage=60&epage=65&date=2010&atitle=Challenge+and+polymorphism+analysis+of+the+novel+A+(H1N1)+influenza+virus+to+normal+animalsen_HK
dc.identifier.emailChen, H:hlchen@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.virusres.2010.03.019en_HK
dc.identifier.pmid20381552-
dc.identifier.scopuseid_2-s2.0-77952741031en_HK
dc.identifier.hkuros180557en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952741031&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume151en_HK
dc.identifier.issue1en_HK
dc.identifier.spage60en_HK
dc.identifier.epage65en_HK
dc.identifier.isiWOS:000278344200009-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridBao, L=35314489100en_HK
dc.identifier.scopusauthoridXu, L=36437938900en_HK
dc.identifier.scopusauthoridZhan, L=25642040300en_HK
dc.identifier.scopusauthoridDeng, W=35314643800en_HK
dc.identifier.scopusauthoridZhu, H=7404664773en_HK
dc.identifier.scopusauthoridGao, H=7402971042en_HK
dc.identifier.scopusauthoridSun, H=36437668000en_HK
dc.identifier.scopusauthoridMa, C=16834878400en_HK
dc.identifier.scopusauthoridLv, Q=36901025600en_HK
dc.identifier.scopusauthoridLi, F=36437162400en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridZhang, L=10241783000en_HK
dc.identifier.scopusauthoridQin, C=7102688076en_HK
dc.identifier.citeulike7064143-

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