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Article: Molecular characterization of in vivo adjuvant activity in ferrets vaccinated against influenza virus
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TitleMolecular characterization of in vivo adjuvant activity in ferrets vaccinated against influenza virus
 
AuthorsFang, Y5 3 1
Rowe, T1 3
Leon, AJ3
Banner, D1
Danesh, A1 5
Xu, L1
Ran, L1
Bosinger, SE4
Guan, Y3
Chen, H3
Cameron, CC1
Cameron, MJ1
Kelvin, DJ5 1 2 3
 
Issue Date2010
 
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
CitationJournal Of Virology, 2010, v. 84 n. 17, p. 8369-8388 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.02305-09
 
AbstractThe 2009 H1N1 influenza pandemic has prompted a significant need for the development of efficient, single-dose, adjuvanted vaccines. Here we investigated the adjuvant potential of CpG oligodeoxynucleotide (ODN) when used with a human seasonal influenza virus vaccine in ferrets. We found that the CpG ODNadjuvanted vaccine effectively increased antibody production and activated type I interferon (IFN) responses compared to vaccine alone. Based on these findings, pegylated IFN-α2b (PEG-IFN) was also evaluated as an adjuvant in comparison to CpG ODN and complete Freund's adjuvant (CFA). Our results showed that all three vaccines with adjuvant added prevented seasonal human A/Brisbane/59/2007 (H1N1) virus replication more effectively than did vaccine alone. Gene expression profiles indicated that, as well as upregulating IFN-stimulated genes (ISGs), CpG ODN enhanced B-cell activation and increased Toll-like receptor 4 (TLR4) and IFN regulatory factor 4 (IRF4) expression, whereas PEG-IFN augmented adaptive immunity by inducing major histocompatibility complex (MHC) transcription and Ras signaling. In contrast, the use of CFA as an adjuvant induced limited ISG expression but increased the transcription of MHC, cell adhesion molecules, and B-cell activation markers. Taken together, our results better characterize the specific molecular pathways leading to adjuvant activity in different adjuvant-mediated influenza virus vaccinations. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
 
ISSN0022-538X
2012 Impact Factor: 5.076
2012 SCImago Journal Rankings: 2.559
 
DOIhttp://dx.doi.org/10.1128/JVI.02305-09
 
PubMed Central IDPMC2919000
 
ISI Accession Number IDWOS:000280605300002
Funding AgencyGrant Number
Li Ka Shing Foundation
Canadian Institute of Health Research
Sardegna Ricerche
National Institutes of Health
Funding Information:

This work is supported by grants from the Li Ka Shing Foundation, the Canadian Institute of Health Research, Sardegna Ricerche, and the National Institutes of Health.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorFang, Y
 
dc.contributor.authorRowe, T
 
dc.contributor.authorLeon, AJ
 
dc.contributor.authorBanner, D
 
dc.contributor.authorDanesh, A
 
dc.contributor.authorXu, L
 
dc.contributor.authorRan, L
 
dc.contributor.authorBosinger, SE
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorChen, H
 
dc.contributor.authorCameron, CC
 
dc.contributor.authorCameron, MJ
 
dc.contributor.authorKelvin, DJ
 
dc.date.accessioned2010-10-31T11:14:14Z
 
dc.date.available2010-10-31T11:14:14Z
 
dc.date.issued2010
 
dc.description.abstractThe 2009 H1N1 influenza pandemic has prompted a significant need for the development of efficient, single-dose, adjuvanted vaccines. Here we investigated the adjuvant potential of CpG oligodeoxynucleotide (ODN) when used with a human seasonal influenza virus vaccine in ferrets. We found that the CpG ODNadjuvanted vaccine effectively increased antibody production and activated type I interferon (IFN) responses compared to vaccine alone. Based on these findings, pegylated IFN-α2b (PEG-IFN) was also evaluated as an adjuvant in comparison to CpG ODN and complete Freund's adjuvant (CFA). Our results showed that all three vaccines with adjuvant added prevented seasonal human A/Brisbane/59/2007 (H1N1) virus replication more effectively than did vaccine alone. Gene expression profiles indicated that, as well as upregulating IFN-stimulated genes (ISGs), CpG ODN enhanced B-cell activation and increased Toll-like receptor 4 (TLR4) and IFN regulatory factor 4 (IRF4) expression, whereas PEG-IFN augmented adaptive immunity by inducing major histocompatibility complex (MHC) transcription and Ras signaling. In contrast, the use of CFA as an adjuvant induced limited ISG expression but increased the transcription of MHC, cell adhesion molecules, and B-cell activation markers. Taken together, our results better characterize the specific molecular pathways leading to adjuvant activity in different adjuvant-mediated influenza virus vaccinations. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal Of Virology, 2010, v. 84 n. 17, p. 8369-8388 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.02305-09
 
dc.identifier.doihttp://dx.doi.org/10.1128/JVI.02305-09
 
dc.identifier.epage8388
 
dc.identifier.hkuros180506
 
dc.identifier.isiWOS:000280605300002
Funding AgencyGrant Number
Li Ka Shing Foundation
Canadian Institute of Health Research
Sardegna Ricerche
National Institutes of Health
Funding Information:

This work is supported by grants from the Li Ka Shing Foundation, the Canadian Institute of Health Research, Sardegna Ricerche, and the National Institutes of Health.

 
dc.identifier.issn0022-538X
2012 Impact Factor: 5.076
2012 SCImago Journal Rankings: 2.559
 
dc.identifier.issue17
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC2919000
 
dc.identifier.pmid20534862
 
dc.identifier.scopuseid_2-s2.0-77956642388
 
dc.identifier.spage8369
 
dc.identifier.urihttp://hdl.handle.net/10722/125150
 
dc.identifier.volume84
 
dc.languageeng
 
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Virology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.
 
dc.rightsCopyright © American Society for Microbiology, Journal of Virology, 2010, v. 84 n. 17, p. 8369-8688
 
dc.subject.meshFerrets
 
dc.subject.meshInfluenza A Virus, H1N1 Subtype - genetics - immunology - physiology
 
dc.subject.meshInfluenza Vaccines - administration and dosage - genetics - immunology
 
dc.subject.meshInfluenza, Human - immunology - prevention and control - virology
 
dc.subject.meshOligodeoxyribonucleotides - administration and dosage - immunology
 
dc.titleMolecular characterization of in vivo adjuvant activity in ferrets vaccinated against influenza virus
 
dc.typeArticle
 
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Author Affiliations
  1. Toronto General Research Institute University of Toronto
  2. Università degli Studi di Sassari
  3. Shantou University, Medical College (SUMC)
  4. University of Pennsylvania
  5. University of Toronto