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- Publisher Website: 10.1099/vir.0.82856-0
- Scopus: eid_2-s2.0-36248965212
- PMID: 17947532
- WOS: WOS:000250795000021
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Article: Severe acute respiratory syndrome coronavirus Orf3a protein interacts with caveolin
Title | Severe acute respiratory syndrome coronavirus Orf3a protein interacts with caveolin |
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Authors | |
Issue Date | 2007 |
Publisher | Society for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org |
Citation | Journal of General Virology, 2007, v. 88 n. 11, p. 3067-3077 How to Cite? |
Abstract | The orf3a (also called X1 or U274) gene is the largest unique open reading frame in the severe acute respiratory syndrome coronavirus genome and has been proposed to encode a protein with three transmembrane domains and a large cytoplasmic domain. Recent work has suggested that the 3a protein may play a structural role in the viral life cycle, although the mechanisms for this remain uncharacterized. Here, the expression of the 3a protein in various in vitro systems is shown, it has been localized to the Golgi region and its membrane topology in transfected cells has been confirmed. Three potential caveolin-1-binding sites were reported to be present in the 3a protein. By using various biochemical, biophysical and genetic techniques, interaction of the 3a protein with caveolin-1 is demonstrated. Any one of the potential sites in the 3a protein was sufficient for this interaction. These results are discussed with respect to the possible roles of the 3a protein in the viral life cycle. © 2007 SGM. |
Persistent Identifier | http://hdl.handle.net/10722/125145 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 0.990 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Padhan, K | en_HK |
dc.contributor.author | Tanwar, C | en_HK |
dc.contributor.author | Hussain, A | en_HK |
dc.contributor.author | Hui, PY | en_HK |
dc.contributor.author | Lee, MY | en_HK |
dc.contributor.author | Cheung, CY | en_HK |
dc.contributor.author | Peiris, JSM | en_HK |
dc.contributor.author | Jameel, S | en_HK |
dc.date.accessioned | 2010-10-31T11:13:56Z | - |
dc.date.available | 2010-10-31T11:13:56Z | - |
dc.date.issued | 2007 | en_HK |
dc.identifier.citation | Journal of General Virology, 2007, v. 88 n. 11, p. 3067-3077 | en_HK |
dc.identifier.issn | 0022-1317 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/125145 | - |
dc.description.abstract | The orf3a (also called X1 or U274) gene is the largest unique open reading frame in the severe acute respiratory syndrome coronavirus genome and has been proposed to encode a protein with three transmembrane domains and a large cytoplasmic domain. Recent work has suggested that the 3a protein may play a structural role in the viral life cycle, although the mechanisms for this remain uncharacterized. Here, the expression of the 3a protein in various in vitro systems is shown, it has been localized to the Golgi region and its membrane topology in transfected cells has been confirmed. Three potential caveolin-1-binding sites were reported to be present in the 3a protein. By using various biochemical, biophysical and genetic techniques, interaction of the 3a protein with caveolin-1 is demonstrated. Any one of the potential sites in the 3a protein was sufficient for this interaction. These results are discussed with respect to the possible roles of the 3a protein in the viral life cycle. © 2007 SGM. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Society for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org | en_HK |
dc.relation.ispartof | Journal of General Virology | en_HK |
dc.subject.mesh | Amino Acid Sequence | - |
dc.subject.mesh | Animals | - |
dc.subject.mesh | Caveolin 1 - metabolism | - |
dc.subject.mesh | SARS Virus - metabolism | - |
dc.subject.mesh | Viral Structural Proteins - metabolism | - |
dc.title | Severe acute respiratory syndrome coronavirus Orf3a protein interacts with caveolin | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lee, MY: suki@hku.hk | en_HK |
dc.identifier.email | Cheung, CY: chungey@hkucc.hku.hk | en_HK |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | en_HK |
dc.identifier.authority | Lee, MY=rp01536 | en_HK |
dc.identifier.authority | Cheung, CY=rp00404 | en_HK |
dc.identifier.authority | Peiris, JSM=rp00410 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1099/vir.0.82856-0 | en_HK |
dc.identifier.pmid | 17947532 | - |
dc.identifier.scopus | eid_2-s2.0-36248965212 | en_HK |
dc.identifier.hkuros | 173445 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-36248965212&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 88 | en_HK |
dc.identifier.issue | 11 | en_HK |
dc.identifier.spage | 3067 | en_HK |
dc.identifier.epage | 3077 | en_HK |
dc.identifier.isi | WOS:000250795000021 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Padhan, K=24740916100 | en_HK |
dc.identifier.scopusauthorid | Tanwar, C=20436960700 | en_HK |
dc.identifier.scopusauthorid | Hussain, A=8957143600 | en_HK |
dc.identifier.scopusauthorid | Hui, PY=36901726500 | en_HK |
dc.identifier.scopusauthorid | Lee, MY=35435155600 | en_HK |
dc.identifier.scopusauthorid | Cheung, CY=7202061836 | en_HK |
dc.identifier.scopusauthorid | Peiris, JSM=7005486823 | en_HK |
dc.identifier.scopusauthorid | Jameel, S=7005029865 | en_HK |
dc.identifier.issnl | 0022-1317 | - |