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Article: Severe acute respiratory syndrome coronavirus Orf3a protein interacts with caveolin

TitleSevere acute respiratory syndrome coronavirus Orf3a protein interacts with caveolin
Authors
Issue Date2007
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal Of General Virology, 2007, v. 88 n. 11, p. 3067-3077 How to Cite?
AbstractThe orf3a (also called X1 or U274) gene is the largest unique open reading frame in the severe acute respiratory syndrome coronavirus genome and has been proposed to encode a protein with three transmembrane domains and a large cytoplasmic domain. Recent work has suggested that the 3a protein may play a structural role in the viral life cycle, although the mechanisms for this remain uncharacterized. Here, the expression of the 3a protein in various in vitro systems is shown, it has been localized to the Golgi region and its membrane topology in transfected cells has been confirmed. Three potential caveolin-1-binding sites were reported to be present in the 3a protein. By using various biochemical, biophysical and genetic techniques, interaction of the 3a protein with caveolin-1 is demonstrated. Any one of the potential sites in the 3a protein was sufficient for this interaction. These results are discussed with respect to the possible roles of the 3a protein in the viral life cycle. © 2007 SGM.
Persistent Identifierhttp://hdl.handle.net/10722/125145
ISSN
2015 Impact Factor: 3.192
2015 SCImago Journal Rankings: 1.741
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPadhan, Ken_HK
dc.contributor.authorTanwar, Cen_HK
dc.contributor.authorHussain, Aen_HK
dc.contributor.authorHui, PYen_HK
dc.contributor.authorLee, MYen_HK
dc.contributor.authorCheung, CYen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorJameel, Sen_HK
dc.date.accessioned2010-10-31T11:13:56Z-
dc.date.available2010-10-31T11:13:56Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of General Virology, 2007, v. 88 n. 11, p. 3067-3077en_HK
dc.identifier.issn0022-1317en_HK
dc.identifier.urihttp://hdl.handle.net/10722/125145-
dc.description.abstractThe orf3a (also called X1 or U274) gene is the largest unique open reading frame in the severe acute respiratory syndrome coronavirus genome and has been proposed to encode a protein with three transmembrane domains and a large cytoplasmic domain. Recent work has suggested that the 3a protein may play a structural role in the viral life cycle, although the mechanisms for this remain uncharacterized. Here, the expression of the 3a protein in various in vitro systems is shown, it has been localized to the Golgi region and its membrane topology in transfected cells has been confirmed. Three potential caveolin-1-binding sites were reported to be present in the 3a protein. By using various biochemical, biophysical and genetic techniques, interaction of the 3a protein with caveolin-1 is demonstrated. Any one of the potential sites in the 3a protein was sufficient for this interaction. These results are discussed with respect to the possible roles of the 3a protein in the viral life cycle. © 2007 SGM.en_HK
dc.languageengen_HK
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_HK
dc.relation.ispartofJournal of General Virologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of General Virology. Copyright © Society for General Microbiology.-
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshCaveolin 1 - metabolism-
dc.subject.meshSARS Virus - metabolism-
dc.subject.meshViral Structural Proteins - metabolism-
dc.titleSevere acute respiratory syndrome coronavirus Orf3a protein interacts with caveolinen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1317&volume=88&issue=Pt 11&spage=3067&epage=3077&date=2007&atitle=Severe+acute+respiratory+syndrome+coronavirus+Orf3a+protein+interacts+with+caveolinen_HK
dc.identifier.emailLee, MY: suki@hku.hken_HK
dc.identifier.emailCheung, CY: chungey@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityLee, MY=rp01536en_HK
dc.identifier.authorityCheung, CY=rp00404en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1099/vir.0.82856-0en_HK
dc.identifier.pmid17947532-
dc.identifier.scopuseid_2-s2.0-36248965212en_HK
dc.identifier.hkuros173445en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36248965212&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume88en_HK
dc.identifier.issue11en_HK
dc.identifier.spage3067en_HK
dc.identifier.epage3077en_HK
dc.identifier.isiWOS:000250795000021-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridPadhan, K=24740916100en_HK
dc.identifier.scopusauthoridTanwar, C=20436960700en_HK
dc.identifier.scopusauthoridHussain, A=8957143600en_HK
dc.identifier.scopusauthoridHui, PY=36901726500en_HK
dc.identifier.scopusauthoridLee, MY=35435155600en_HK
dc.identifier.scopusauthoridCheung, CY=7202061836en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridJameel, S=7005029865en_HK

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